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Secreted or surface-exposed proteins play a critical role in disease because they often mediate interactions between host and pathogen. In the present work, we sought to identify possible<jats:italic>M. synoviae<\/jats:italic>secreted proteins by cultivating the bacteria in a modified protein-free Frey medium. Using this approach, we were able to detect in the cell-free fraction a number of proteins that have been shown in other organisms to be secreted, suggesting that they may also be secreted by<jats:italic>M. synoviae<\/jats:italic>.<\/jats:p>","DOI":"10.5402\/2012\/802308","type":"journal-article","created":{"date-parts":[[2012,9,6]],"date-time":"2012-09-06T21:42:48Z","timestamp":1346967768000},"page":"1-6","source":"Crossref","is-referenced-by-count":15,"title":["Extracellular Proteins of<i>Mycoplasma synoviae<\/i>"],"prefix":"10.5402","volume":"2012","author":[{"given":"Manuel Sebasti\u00e1n","family":"Rebollo Couto","sequence":"first","affiliation":[{"name":"Centro de Biologia Molecular Estrutural, Departamento de Bioqu\u00edmica, CCB, Universidade Federal de Santa Catarina, 88040-900 Florian\u00f3polis, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catia Silene","family":"Klein","sequence":"additional","affiliation":[{"name":"Embrapa Swine and Poultry 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