{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:16:29Z","timestamp":1792790189270,"version":"4.5.0"},"reference-count":37,"publisher":"Korean Society of Plant Pathology","issue":"5","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["RS-2022-RD010350"],"award-info":[{"award-number":["RS-2022-RD010350"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["RS-2025-02183115"],"award-info":[{"award-number":["RS-2025-02183115"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Science and ICT","award":["RS-2025-00555346"],"award-info":[{"award-number":["RS-2025-00555346"]}],"id":[{"id":"https:\/\/ror.org\/01wpjm123","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["ppjonline.org"],"crossmark-restriction":true},"short-container-title":["Plant Pathol J"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:p>Botrytis cinerea, the causal agent of gray mold, is a major pathogen in strawberry production. Quinone outside inhibitor (QoI) fungicides have been widely used for disease control, but resistance driven by the G143A mutation in cytochrome b (cytb) has limited their field efficacy. This study evaluated the sensitivity of 229 B. cinerea isolates collected from farms in major Korean strawberry-growing regions during 2022\u20132024 to the QoI fungicide pyraclostrobin. The EC50 values for pyraclostrobin ranged from 0.10 to 329.21 \u03bcg mL-1. In total, 86.46% of the population showed resistance to pyraclostrobin, harboring the G143A mutation in cytb. In planta and greenhouse assays confirmed that pyraclostrobin failed to control the QoI-resistant isolates. Furthermore, strong cross-resistance to pyraclostrobin, mandestrobin, and pyribencarb indicates that QoI-resistant populations substantially limit the field efficacy of QoI fungicides. To assess how the QoI resistance mutation affects selection under non-QoI fungicide pressure, mixed QoI-sensitive and -resistant isolates were treated with alternative fungicides. Allele-specific qPCR revealed that pydiflumetofen, fludioxonil, and fluxapyroxad maintained a higher proportion of the QoI-sensitive genotype at all concentrations, whereas pyraclostrobin consistently favored the resistant genotype. These findings indicated that QoI fungicides impose strong selection pressure promoting resistant B. cinerea populations, while non-QoI fungicides help maintain sensitive individuals. Our study suggests the need for fungicide class rotation and the targeted use of non-QoI fungicides to delay QoI resistance development and preserve the efficacy of gray mold management in strawberry production.<\/jats:p>","DOI":"10.5423\/ppj.oa.04.2026.0063","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T06:05:59Z","timestamp":1787637959000},"page":"673-685","update-policy":"https:\/\/doi.org\/10.5423\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence of Quinone Outside Inhibitor Fungicide Resistance and Dynamics of Cytochrome b Genotypes Under Different Fungicide Selection Pressure in Botrytis cinerea Isolates from Korean Strawberry Farms"],"prefix":"10.5423","volume":"42","author":[{"given":"Hyeong-Rok","family":"Jang","sequence":"first","affiliation":[{"name":"Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, 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