{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:50:14Z","timestamp":1791427814182,"version":"4.3.3"},"reference-count":57,"publisher":"Korean Society of Plant Pathology","issue":"5","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007288","name":"Mahasarakham University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007288","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science Classroom in University Affiliated School"}],"content-domain":{"domain":["ppjonline.org"],"crossmark-restriction":true},"short-container-title":["Plant Pathol J"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:p>Yeasts associated with medicinal-plant tissues are potential sources of antifungal compounds for plant disease management. Microorganisms isolated from surface-sterilised rhizomes of Boesenbergia rotunda and Alpinia galanga were screened against Fusarium oxysporum f. sp. lycopersici (Fol), F. oxysporum f. sp. cubense (Foc), and Sclerotium rolfsii. In dual-culture assays, BRKS1-2 showed the highest mean inhibition across pathogens (35.5%; p &lt; 0.001), including 42.2 \u00b1 5.3% against Foc and 39.0 \u00b1 0.9% against S. rolfsii, and was the only isolate active against S. rolfsii. Cellfree culture filtrates collected after 24\u201372 h inhibited all pathogens, with activity increasing over time (p &lt; 0.001). ITS and LSU rRNA phylogenies placed BRKS1-2 within Nakaseomyces, although ITS did not resolve species identity. Whole-genome sequencing produced an 11.5-Mb draft assembly (37.2% GC) at 185.8\u00d7 mean mapped depth, with 99.90% of filtered reads mapped. Genome-wide comparison showed 98.3% average nucleotide identity with the N. kungkrabaensis reference, supporting identification as N. kungkrabaensis. Gene prediction identified 5,112 protein-coding genes, 222 tRNA genes, and five rRNA genes. LC-MS\/MS detected 74 fungal protein groups, mainly associated with central metabolism and stress responses. Fungal-mode antiSMASH predicted four terpene-related clusters and one putative beta-lactone cluster; all abutted contig boundaries and were therefore partial. These findings demonstrate the in vitro antifungal activity of BRKS1-2 and identify candidate biosynthetic loci for investigating the responsible compounds.<\/jats:p>","DOI":"10.5423\/ppj.oa.07.2026.0097","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:46:30Z","timestamp":1790811990000},"page":"738-753","update-policy":"https:\/\/doi.org\/10.5423\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Whole-Genome Analysis Reveals Biosynthetic Potential and Antifungal Activity of Nakaseomyces kungkrabaensis BRKS1-2 Isolated from Boesenbergia rotunda Rhizome"],"prefix":"10.5423","volume":"42","author":[{"given":"Praphat","family":"Kawicha","sequence":"first","affiliation":[{"name":"Plant Genome and Disease Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, 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