{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T08:23:17Z","timestamp":1775377397841,"version":"3.50.1"},"reference-count":11,"publisher":"Editorial CSIC","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["For. syst."],"abstract":"<jats:p>Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis.Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain).Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage.Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages.Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g\/L Gelrite\u00ae at 23\u00baC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18\u00baC in a culture media with significantly lower water availability.\u00a0<\/jats:p>","DOI":"10.5424\/fs\/2017263-11436","type":"journal-article","created":{"date-parts":[[2017,11,13]],"date-time":"2017-11-13T11:40:21Z","timestamp":1510573221000},"page":"eSC05","source":"Crossref","is-referenced-by-count":9,"title":["Short communication: The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis"],"prefix":"10.5424","volume":"26","author":[{"given":"Catia","family":"Pereira","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1868-5058","authenticated-orcid":false,"given":"Itziar A.","family":"Montalb\u00e1n","sequence":"additional","affiliation":[]},{"given":"Tom\u00e1s","family":"Goicoa","sequence":"additional","affiliation":[]},{"given":"M. Dolores","family":"Ugarte","sequence":"additional","affiliation":[]},{"given":"Sandra","family":"Correia","sequence":"additional","affiliation":[]},{"given":"Jorge M.","family":"Canhoto","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5816-0830","authenticated-orcid":false,"given":"Paloma","family":"Moncale\u00e1n","sequence":"additional","affiliation":[]}],"member":"2373","published-online":{"date-parts":[[2017,12,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1007\/s00468-015-1336-7"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaridenv.2009.05.005"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1469-8137.2007.02222.x","article-title":"Timing of bud set in Picea abies is regulated by a memory of temperature during zygotic and somatic embryogenesis.","volume":"177","author":"Kvaalen","year":"2008","unstructured":"Kvaalen H, Johnsen \u00d8, 2008. Timing of bud set in Picea abies is regulated by a memory of temperature during zygotic and somatic embryogenesis. New Phytol 177: 49-59.","journal-title":"New Phytol","ISSN":"https:\/\/id.crossref.org\/issn\/0028-646X","issn-type":"print"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.foreco.2004.05.040"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s11738-011-0841-6"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00468-013-0882-0"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007\/s11056-014-9457-1"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.12158"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1007\/s10310-016-0524-7"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s11056-014-9407-y"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.2010.01423.x"}],"container-title":["Forest Systems"],"original-title":[],"link":[{"URL":"https:\/\/fs.revistas.csic.es\/index.php\/fs\/article\/download\/11436\/3709","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/fs.revistas.csic.es\/index.php\/fs\/article\/download\/11436\/3759","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/fs.revistas.csic.es\/index.php\/fs\/article\/download\/11436\/3750","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/fs.revistas.csic.es\/index.php\/fs\/article\/download\/11436\/3709","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T08:51:31Z","timestamp":1747817491000},"score":1,"resource":{"primary":{"URL":"https:\/\/fs.revistas.csic.es\/index.php\/fs\/article\/view\/11436"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,30]]},"references-count":11,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2017,12,30]]}},"URL":"https:\/\/doi.org\/10.5424\/fs\/2017263-11436","relation":{},"ISSN":["2171-9845","2171-5068"],"issn-type":[{"value":"2171-9845","type":"electronic"},{"value":"2171-5068","type":"print"}],"subject":[],"published":{"date-parts":[[2017,12,30]]}}}