{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:01:03Z","timestamp":1791482463445,"version":"4.3.4"},"reference-count":0,"publisher":"ScopeMed","issue":"3","license":[{"start":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T00:00:00Z","timestamp":1662768000000},"content-version":"vor","delay-in-days":252,"URL":"http:\/\/www.actainformmed.org\/\/?sec=licenseinfo"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Inform Med"],"published-print":{"date-parts":[[2022]]},"abstract":"<jats:p>Background: Infertility is a serious health issue that affects people all around the world. One of the most common reasons for male infertility is sperm abnormalities. Researchers and scientists have been searching for a novel genetic marker to detect or recognize the genetic malfunction that causes sperm abnormalities. Micro-RNA (miRNAs) are small non-coded RNA molecules that present intra and extra-cellular and regulate gene expression. Objective: This studies began to search for a relation between miRNA expression levels and other diseases that may be related to them, considering that the main role of miRNAs was the down-regulation of genes. Methods: The main technique used in this study was to synthesize a complementary DNA (cDNA) (revers transcription method) of extracted total RNA by TRIzol then amplification of candidates\u2019 miRNAs genes by Reverse Transcriptase Quantitative Polymerase Chain Reaction RT-qPCR. Results: Studies found that miRNAs have a role in defining sperm qualities such as sperm count, motility, and shape. In this study, we chose the most miRNAs referred to in the previous study as a potential seminal fluid marker (miR-10a, miR-10b, miR-135a and miR-135b) to test them as potential infertility-related miRNAs markers (Asthenospermia AS, Oligospermia OS, Astheno-Oligospermia ASOS) in addition to health normal sperm NS. Conclusion: the main aim of this study was to find the miRNAs expression pattern to find a way to help scientists track the genetic causes of male infertility issues and a novel method to distinguish infertility genetically diseases. Conclusion: The findings may serve as a potential genetic marker for male infertility and provide a background for future research that targeted miRNAs as a molecular marker for medical and forensic fields, also as an infertility disease potential treatment.<\/jats:p>","DOI":"10.5455\/aim.2022.30.205-212","type":"journal-article","created":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T17:23:25Z","timestamp":1662830605000},"page":"205","source":"Crossref","is-referenced-by-count":5,"title":["Study of Small Non-Coding RNA (miRNA) Expression Pattern of Fertile\/Infertile Male Semen"],"prefix":"10.5455","volume":"30","author":[{"given":"Yasir","family":"Mawlah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Darraji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Imari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3407","container-title":["Acta Informatica Medica"],"original-title":[],"link":[{"URL":"https:\/\/ejmanager.com\/mnstemps\/6\/6-1662637133.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T17:48:04Z","timestamp":1791481684000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ejmanager.com\/mnstemps\/6\/6-1662637133.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.5455\/aim.2022.30.205-212","relation":{},"ISSN":["0353-8109"],"issn-type":[{"value":"0353-8109","type":"print"}],"subject":[],"published":{"date-parts":[[2022]]}}}