{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:06:00Z","timestamp":1791486360239,"version":"4.3.4"},"reference-count":0,"publisher":"ScopeMed","issue":"2","license":[{"start":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T00:00:00Z","timestamp":1720915200000},"content-version":"vor","delay-in-days":195,"URL":"https:\/\/bdvets.org\/JAVAR\/?sec=licenseinfo"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Adv Vet Anim Res"],"published-print":{"date-parts":[[2024]]},"abstract":"<jats:p>Objective: There is still much to be discovered regarding the etiopathogenesis and management of polycystic ovarian syndrome (PCOS). \nMaterials and Methods: Four groups of female Wister-Albino rats were established, each with a normal estrous cycle: control, D ( + ) galactose (D-galactose), Lepidium sativum (L. sativum), and prepared secondary antibody (Ab2). Serum samples were collected, and histopathological examination was performed on ovaries and spleen tissues. Immunoreactive anti-ovarian antibody (AOA) quantities were determined using a modified antigen-based ELISA procedure. ELISA assay kits were used to quantify FSH, LH, and estradiol 17 \u03b2 concentrations. \nResults: The study found that AOA concentration in undiluted samples was significantly higher in the second and fourth weeks after PCOS induction by D-galactose (p &lt; 0.001). However, antibody index% and titer elevated in the D-galactose group. L. sativum\u2019s late efficacy was observed in the fourth week, while the concentration of undiluted samples in the D-galactose + Ab2 group lowered (p &lt; 0.001). Higher basal FSH and LH levels and lower estrogen levels are associated with PCOS development. L. sativum\u2019s immunomodulatory properties may contribute to this associa\u00action. Estradiol-17\u00df concentrations increased in D-galactose + L. sativum and D-galactose + Ab2 groups, respectively. \nConclusion: Careful extrapolation of experimental models is crucial for clinical applications, as technical advancements make Ab2 production easier. Further study is needed to fully understand its potential in immunotherapy.<\/jats:p>","DOI":"10.5455\/javar.2024.k791","type":"journal-article","created":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T05:30:55Z","timestamp":1720935055000},"page":"418","source":"Crossref","is-referenced-by-count":2,"title":["Treatment of D-galactose-induced rat polycystic ovarian condition using Lepidium sativum and secondary antibodies"],"prefix":"10.5455","volume":"11","author":[{"given":"Yousef","family":"Alharbi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulrahman","family":"Alkhail","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdel","family":"Zaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3407","container-title":["Journal of Advanced Veterinary and Animal Research"],"original-title":[],"link":[{"URL":"https:\/\/ejmanager.com\/mnstemps\/39\/39-1694508691.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:02:20Z","timestamp":1791482540000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ejmanager.com\/mnstemps\/39\/39-1694508691.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.5455\/javar.2024.k791","relation":{},"ISSN":["2311-7710"],"issn-type":[{"value":"2311-7710","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]}}}