{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:55:03Z","timestamp":1791975303155,"version":"4.4.0"},"reference-count":0,"publisher":"ScopeMed","issue":"3","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"vor","delay-in-days":305,"URL":"https:\/\/bdvets.org\/JAVAR\/?sec=licenseinfo"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Adv Vet Anim Res"],"published-print":{"date-parts":[[2024]]},"abstract":"<jats:p>Objective: The objective was to find out the expression of EphB4 receptor and ephrin-B1 ligand by the macrophages that live inside the mouse testicles. \nMaterials and Methods: Messenger ribonucleic acid (mRNA) expression of EphB4 and ephrin-B1 was identified via RT-PCR amplification, and protein expression was examined by immunostaining. \nResults: Analysis using RT-PCR revealed that mRNA of EphB4 and ephrin-B1 were noticed in the examined testis of all postnatal ages. Furthermore, immunostaining revealed that F4\/80-positive intra-testicular-resident macrophages were located in the intertubular spaces within the testis and more densely around the intra-testicular excurrent duct system, and increased in number gradually during the postnatal period of development until 5 weeks of age, when the mice attain their maturity (puberty), and maintained thereafter. Both EphB4 and ephrin-B1 immunoreactiv\u00acity were noticed in F4\/80-positive intra-testicular-resident macrophages within the testis of all studied postnatal ages. Ephrin-B1 and EphB4 immunoreactivity were weak during early postnatal development until the age of 2 weeks, and then ephrin-B1 immunoreactivity became very strong and EphB4 immunoreactivity became strong at the age of 3 weeks, and they continued to do so until the age of 8 weeks. Furthermore, EphB4 receptor was tyrosine-phosphorylated in testis. \nConclusion: The expression of EphB4 and ephrin-B1 in mice intra-testicular-resident macro\u00acphages is being examined for the first time in this work. The localization of EphB4 and ephrin-B1, and EphB4 tyrosine-phosphorylation suggest that EphB4\/ephrin-B1 signaling might occur in the intra-testicular-resident macrophages, and may participate in maintaining male fertility.<\/jats:p>","DOI":"10.5455\/javar.2024.k826","type":"journal-article","created":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T12:25:52Z","timestamp":1730463952000},"page":"746","source":"Crossref","is-referenced-by-count":2,"title":["EphB4 and ephrin-B1 expression in the intra-testicular-resident macrophages in mice"],"prefix":"10.5455","volume":"11","author":[{"given":"Md","family":"Gofur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazushige","family":"Ogawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3407","container-title":["Journal of Advanced Veterinary and Animal Research"],"original-title":[],"link":[{"URL":"https:\/\/ejmanager.com\/mnstemps\/39\/39-1719133350.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:02:27Z","timestamp":1791482547000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ejmanager.com\/mnstemps\/39\/39-1719133350.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024]]}},"URL":"https:\/\/doi.org\/10.5455\/javar.2024.k826","relation":{},"ISSN":["2311-7710"],"issn-type":[{"value":"2311-7710","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]}}}