{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:06:23Z","timestamp":1791486383593,"version":"4.3.4"},"reference-count":0,"publisher":"ScopeMed","issue":"1","license":[{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"vor","delay-in-days":111,"URL":"https:\/\/bdvets.org\/JAVAR\/?sec=licenseinfo"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Adv Vet Anim Res"],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p>Objective: This study aimed to identify the impact of variations in solvent polarity on the solu-bility of secondary metabolite compounds, which were correlated as antioxidant and antibacte-rial agents for &lt;i&gt;Escherichia coli&lt;\/i&gt; (ATCC 11725), &lt;i&gt;Salmonella&lt;\/i&gt; sp. (ATCC 22504), &lt;i&gt;Staphylococcus aureus&lt;\/i&gt; (ATCC 11526), and &lt;i&gt;Bacillus subtilis&lt;\/i&gt; (ATCC 11626). \nMaterials and Methods: A total of 500 gm of Temulawak flour (&lt;i&gt;Curcuma xanthorrhiza&lt;\/i&gt;) was macerated gradually using the step gradient polarity (SGP) technique, where the solvents (methanol, ethyl acetate, and n-hexane) used had different levels of polarity. The extracting process of secondary metabolite components of Temulawak flour began with the use of non-polar solvents, semipolar solvents, and polar solvents, respectively. Each soaking process was completed for 5 \u00d7 24 h. \nResults: From the Temulawak extract with different levels of solvent polarity, 76.048 gm were obtained for the methanol fraction, 106.242 gm for the ethyl acetate fraction, and 154.575 gm for the n-hexane fraction. The results of the antioxidant activity test showed that the ethyl acetate fraction had the highest inhibitory value for antioxidant activity of 50% (IC50) 22.59 mg\/l. The results of this research\u2019s &lt;i&gt;\u03b2&lt;\/i&gt;-carotene content were 0.0865 gm. Ethyl acetate fraction was known to have good activity from the antibacterial activity test. The inhibition zone of &lt;i&gt;E. coli&lt;\/i&gt; and &lt;i&gt;B. subtilis&lt;\/i&gt; bacteria was 11.5 \u00b1 0.71 with a minimum concentration of 3.13 mg\/ml. The inhibition zone of &lt;i&gt;S. aureus&lt;\/i&gt; was 10.5 \u00b1 0.17 with a minimum inhibitory concentration (MIC) of 6.25 mg\/ml, while the inhibition zone for &lt;i&gt;Salmonella&lt;\/i&gt; sp. was 8.125 \u00b1 0.35 with a MIC of 37.50 mg\/ml. \nConclusion: All fractions have moderate antibacterial activity, yet the ability of the ethyl acetate fraction of Temulawak extract was higher than the methanol and n-hexane fractions.<\/jats:p>","DOI":"10.5455\/javar.2025.l886","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T18:11:12Z","timestamp":1745345472000},"page":"192","source":"Crossref","is-referenced-by-count":3,"title":["The phenolic and flavonoid content and biological activity of Curcuma (&lt;i&gt;Curcuma xanthorrhiza&lt;\/i&gt;) fractions with different solvent polarities."],"prefix":"10.5455","volume":"12","author":[{"given":"Ucop","family":"Haroen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syafwan","family":"Syafwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiki","family":"Kurniawan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agus","family":"Budiansyah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilawati","family":"Widjaja","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saitul","family":"Fakhri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3407","container-title":["Journal of Advanced Veterinary and Animal Research"],"original-title":[],"link":[{"URL":"https:\/\/www.ejmanager.com\/mnstemps\/39\/39-1714444686.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:02:39Z","timestamp":1791482559000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ejmanager.com\/mnstemps\/39\/39-1714444686.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.5455\/javar.2025.l886","relation":{},"ISSN":["2311-7710"],"issn-type":[{"value":"2311-7710","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}