{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T16:27:24Z","timestamp":1792686444259,"version":"4.5.0"},"reference-count":0,"publisher":"ScopeMed","issue":"1","license":[{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"vor","delay-in-days":111,"URL":"https:\/\/bdvets.org\/JAVAR\/?sec=licenseinfo"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Adv Vet Anim Res"],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p>Objectives: The rise of antibiotic-resistant strains of &lt;i&gt;Staphylococcus aureus&lt;\/i&gt; in dairy milk products is a global concern, compromising treatment efficacy and highlighting the need for innovative solutions. Therefore, a study was conducted to isolate &lt;i&gt;S. aureus&lt;\/i&gt; strains (N = 21) from raw milk samples of cows infected with subclinical bovine mastitis. Additionally, the resistance of these strains against 12 different antibiotics was examined. \nMaterials and Methods: Sixty raw cow milk samples, 20 from each of three separate dairy farms in Lahore city, were collected and screened for the presence of &lt;i&gt;S. aureus&lt;\/i&gt;. It was discovered that 70% of these milk samples were contaminated with this bacterium, indicating a widespread presence across the farms. Different isolation tests were employed in this study, including gram staining, capsule staining, catalase, mannitol salt fermentation, DNase, coagulase, and oxidase. \nResults: The obtained results revealed that the isolated strains of &lt;i&gt;S. aureus&lt;\/i&gt; showed % of their resistance against different antibiotics in the order of amoxicillin (85%), penicillin (71%), gentamicin (CN) (42%), carbenicillin and trimethoprim-sulfamethoxazole (33%), streptomycin, ciprofloxacin, and oxytetracycline (28%), cefotaxime (10%), and chloramphenicol (4%) in decreasing order, respectively. However, these strains showed no resistance against Bacitracin and Ampicillin. \nConclusion: The existence of resistant strains of &lt;i&gt;S. aureus&lt;\/i&gt; has been attributed to various factors, such as poor milk hygiene, delayed milk transportation, subclinical bovine mastitis among dairy cows, and antibiotic-resistant genes. Thus, our present study will provide useful information about the resistant strains of &lt;i&gt;S. aureus&lt;\/i&gt;, which may transfer through cows into milk and then produce serious food-borne diseases in human beings. This study will be helpful to improve and control the quality of dairy products in Pakistan.<\/jats:p>","DOI":"10.5455\/javar.2025.l892","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T22:11:12Z","timestamp":1745359872000},"page":"252","source":"Crossref","is-referenced-by-count":7,"title":["Isolation of antibiotic-resistant strains of Staphylococcus aureus from raw milk produced by dairy cows with subclinical bovine mastitis."],"prefix":"10.5455","volume":"12","author":[{"given":"Saima","family":"Batool","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zubia","family":"Masood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asim","family":"Ullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wali","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mourad","family":"Said","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Belkahia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaa","family":"Ismael","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayman","family":"Swelum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3407","container-title":["Journal of Advanced Veterinary and Animal Research"],"original-title":[],"link":[{"URL":"https:\/\/www.ejmanager.com\/mnstemps\/39\/39-1715177337.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T22:11:24Z","timestamp":1745359884000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ejmanager.com\/fulltextpdf.php?mno=200919"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.5455\/javar.2025.l892","relation":{},"ISSN":["2311-7710"],"issn-type":[{"value":"2311-7710","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}