{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:06:24Z","timestamp":1791486384478,"version":"4.3.4"},"reference-count":0,"publisher":"ScopeMed","issue":"2","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"vor","delay-in-days":212,"URL":"https:\/\/bdvets.org\/JAVAR\/?sec=licenseinfo"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Adv Vet Anim Res"],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p>&lt;b&gt;Objectives:&lt;\/b&gt; This study aimed to assess the anti-methanogenic potential of &lt;i&gt;Moringa oleifera&lt;\/i&gt; L. phytogenic extracts through &lt;i&gt;in silico&lt;\/i&gt; inhibition of the methyl-coenzyme M reductase (MCR) receptor. \n&lt;b&gt;Materials and Methods:&lt;\/b&gt; Phytochemicals from &lt;i&gt;M. oleifera&lt;\/i&gt; were screened and compared with anti-methanogenic compounds such as 3-nitrooxypropanol and native MCR enzyme ligands (coenzyme M and coenzyme B). Molecular docking analysis was performed using AutoDock Vina on PyRx 0.8, and interactions were visualized with Discovery Studio 2024. \n&lt;b&gt;Results:&lt;\/b&gt; Selected phytochemicals, including pterygospermin, exhibited promising drug-likeness based on Lipinski\u2019s rule of five and absorption, distribution, metabolism, excretion, and toxicology properties. Pterygospermin demonstrated the highest binding affinity to the MCR enzyme\u2019s active site, with interactions including Pi-sulfur (Phe443), Pi-alkyl (Val482, Leu320, and Met324), Pi-Pi stacking (Phe330), and van der Waals forces (Tyr333 and Ser325). \n&lt;b&gt;Conclusion:&lt;\/b&gt; Pterygospermin shows potential as a competitive inhibitor of the MCR enzyme, providing a sustainable approach to mitigate methane emissions in livestock and contribute to global greenhouse gas reduction efforts.<\/jats:p>","DOI":"10.5455\/javar.2025.l904","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T23:30:41Z","timestamp":1754091041000},"page":"361","source":"Crossref","is-referenced-by-count":1,"title":["Anti-methanogenic effect of phytogenic extract of &lt;i&gt;Moringa oleifera&lt;\/i&gt; on methane mitigation through inhibition of methyl-coenzyme M reductase receptor: &lt;i&gt;In silico&lt;\/i&gt; study"],"prefix":"10.5455","volume":"12","author":[{"given":"Muhammad","family":"Daulai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indah","family":"Wijayanti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuli","family":"Retnani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzuki","family":"Toshisada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3407","container-title":["Journal of Advanced Veterinary and Animal Research"],"original-title":[],"link":[{"URL":"https:\/\/www.bibliomed.org\/fulltextpdf.php?mno=236018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:02:42Z","timestamp":1791482562000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bibliomed.org\/?mno=236018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.5455\/javar.2025.l904","relation":{},"ISSN":["2311-7710"],"issn-type":[{"value":"2311-7710","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]}}}