{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T15:28:36Z","timestamp":1794324516724,"version":"4.9.0"},"reference-count":0,"publisher":"ScopeMed","issue":"1","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":130,"URL":"https:\/\/bdvets.org\/JAVAR\/?sec=licenseinfo"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Adv Vet Anim Res"],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>&lt;b&gt;Objectives:&lt;\/b&gt; &lt;i&gt;Fructus Mume&lt;\/i&gt; has long been used to prevent diseases by modulating gut microbiota, enhancing intestinal barrier integrity, and boosting immune function. Therefore, it is necessary to clarify the specific connection between &lt;i&gt;Fructus Mume&lt;\/i&gt; and gut health.\n&lt;b&gt;Materials and Methods:&lt;\/b&gt; This study employed &lt;i&gt;16S rDNA&lt;\/i&gt; sequencing to investigate the regulatory effects of &lt;i&gt;Fructus Mume&lt;\/i&gt; decoction on the gut microbiota structure in Kunming (KM) mice. 13-week-old male KM mice were randomly assigned to a control group and to low-, medium-, and high-dose &lt;i&gt;Fructus Mume&lt;\/i&gt; groups (&lt;i&gt;n&lt;\/i&gt; = 4 per group). Mice were administered distilled water or&lt;i&gt; Fructus Mume&lt;\/i&gt; decoction (0.42, 0.84, 1.68 gm\/kg) via daily gavage (0.2 ml) for 7 consecutive days. Fecal samples were collected on days 1 and 7 post-withdrawal.\n&lt;b&gt;Results:&lt;\/b&gt; At the phylum level, on day 1 post-withdrawal, the Bacteroidota abundance increased slightly in the high-dose group, while the Firmicutes_D abundance increased significantly. At the genus level, CAG-873 and Alloprevotella exhibited significantly positive correlations with &lt;i&gt;Fructus Mume &lt;\/i&gt;dose, whereas UMGS1994 and Nanosyncoccus showed significantly negative correlations (|&lt;i&gt;r&lt;\/i&gt;| &gt; 0.4). Functional prediction indicated upregulation of carbohydrate metabolism and xenobiotic biodegradation and metabolism pathways in &lt;i&gt;Fructus Mume&lt;\/i&gt; groups. The latter pathway gradually downregulated post-treatment cessation. Molecular docking analysis revealed that kaempferol and quercetin in &lt;i&gt;Fructus Mume&lt;\/i&gt; decoction could interact with core proteins associated with intestinal diseases.\n&lt;b&gt;Conclusions:&lt;\/b&gt; Gavage of Fructus Mume decoction at human-equivalent doses for one week altered intestinal flora composition in KM mice, reducing diversity and richness, and the dose of 0.84 gm\/kg was more beneficial to gut health. These parameters gradually recovered post-withdrawal.<\/jats:p>","DOI":"10.5455\/javar.2026.m1034","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T19:57:16Z","timestamp":1778529436000},"page":"319","source":"Crossref","is-referenced-by-count":0,"title":["Regulatory effect of &lt;i&gt;Fructus Mume&lt;\/i&gt; decoction on gut microbiota in mice based on &lt;i&gt;16S rDNA&lt;\/i&gt; sequencing"],"prefix":"10.5455","volume":"13","author":[{"given":"Jianhui","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongjie","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenfeng","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peifu","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3407","container-title":["Journal of Advanced Veterinary and Animal Research"],"original-title":[],"link":[{"URL":"https:\/\/www.bibliomed.org\/fulltextpdf.php?mno=285180","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:03:06Z","timestamp":1791482586000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bibliomed.org\/?mno=285180"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.5455\/javar.2026.m1034","relation":{},"ISSN":["2311-7710"],"issn-type":[{"value":"2311-7710","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}