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Isolation and molecular detection"},{"key":"ref3","unstructured":"of pigeon pox virus in Assiut and New Valley"},{"key":"ref4","unstructured":"governorates. J. Virol. Methods. 293, 114142."},{"key":"ref5","unstructured":"Abodalal, S.E.S.A., Hafez, M.S.H.A., Abd El-munem"},{"key":"ref6","unstructured":"Shosha, E., Warda, F.F. and Hagag, N.M. 2021."},{"key":"ref7","unstructured":"Isolation and Molecular Characterization of Rabbit"},{"key":"ref8","unstructured":"Haemorrhagic Disease Virus Strains Circulating"},{"key":"ref9","unstructured":"in Rabbit Population Using Sequencing and"},{"key":"ref10","unstructured":"Phylogenetic Analysis. J. World. Poultry. Res."},{"key":"ref11","unstructured":"11(3), 302\u2013311."},{"key":"ref12","unstructured":"Ahmed, D.A.A., Hassan, A.K., Ali, D.A.A., Shosha,"},{"key":"ref13","unstructured":"E.A.E., Eldaghayes, I.M. and Khair, S.M.A.A."},{"key":"ref14","unstructured":"2025. Molecular detection and sequencing of the"},{"key":"ref15","unstructured":"NetB toxin Gene of clostridium perfringens and"},{"key":"ref16","unstructured":"evaluation of its pathogenicity in broiler chicken."},{"key":"ref17","unstructured":"Poult. Sci. 105(2), 106289."},{"key":"ref18","unstructured":"Al-Hammadi, M.A. and Al-Rasheed, M. 2024."},{"key":"ref19","unstructured":"Occurrence of avian encephalomyelitis virus in"},{"key":"ref20","unstructured":"naturally infected chicks in Saudi Arabia\u2019s Eastern"},{"key":"ref21","unstructured":"Province. Open Vet. J. 14, 335\u2013340."},{"key":"ref22","unstructured":"Ali, M., Shaon, M., Moula, M., Bary, M., Sabuj,"},{"key":"ref23","unstructured":"A., Khaled, S., Bhuiyan, Z. and Giasuddin, M."},{"key":"ref24","unstructured":"2021. First report on the seroprevalence of avian"},{"key":"ref25","unstructured":"encephalomyelitis virus antibody in Sonali (cross-"},{"key":"ref26","unstructured":"bred) chickens in Bogura, Bangladesh. J. Adv. Vet."},{"key":"ref27","unstructured":"Anim. Res. 8, 78\u201383."},{"key":"ref28","unstructured":"Amal, A.M., Zaghloul, M., El-Munem, S.E.A.,"},{"key":"ref29","unstructured":"Darwish, D.M., Heba A Khafagy., Farouk, E.M. and"},{"key":"ref30","unstructured":"Ibrahim, A.I. 2019. Cross neutralization between"},{"key":"ref31","unstructured":"vaccinal strain of commercial bovine ephemeral"},{"key":"ref32","unstructured":"fever virus vaccines and Egyptian isolate 2018 with"},{"key":"ref33","unstructured":"serum samples of vaccinated cattle. Inter. J. Vet."},{"key":"ref34","unstructured":"Sci. 8(4), 329\u2013334."},{"key":"ref35","unstructured":"Badr, H., Abdelmenamm Shosha, E., Roshdy, H.,"},{"key":"ref36","unstructured":"Abd El-halem Mohammed, A., Saad, N., Mostafa"},{"key":"ref37","unstructured":"Aboelenin, S., Mohamed Soliman, M., El-Tahan,"},{"key":"ref38","unstructured":"A.M., El-Saadony, M.T. and Yehia, N. 2022."},{"key":"ref39","unstructured":"Investigation of many bacterial and viral infections"},{"key":"ref40","unstructured":"circulating in pigeons showing nervous symptoms."},{"key":"ref41","unstructured":"Saudi J. Biol. Sci. 29(4), 2911\u20132920."},{"key":"ref42","unstructured":"Biswas, S.K., Jana, C., Chand, K., Rehman, W. and"},{"key":"ref43","unstructured":"Mondal, B. 2011. Detection of fowl poxvirus"},{"key":"ref44","unstructured":"integrated with reticuloendotheliosis virus"},{"key":"ref45","unstructured":"sequences from an outbreak in backyard chickens"},{"key":"ref46","unstructured":"in India. Veterinaria. Italiana. 47, 147\u2013153."},{"key":"ref47","unstructured":"Braune, M.O. and Gentry, R.F. 1971. Avian"},{"key":"ref48","unstructured":"encephalomyelitis virus. I. Pathogenesis in chicken"},{"key":"ref49","unstructured":"embryos. Avian Dis. 15, 638\u2013647."},{"key":"ref50","unstructured":"Diab, M.S., Abd El Hafez, M.S., Ashry, M.A. and"},{"key":"ref51","unstructured":"Elfeil, W.K. 2019. Occurrence of avian influenza"},{"key":"ref52","unstructured":"H5N1 among chicken, duck, farms and human in"},{"key":"ref53","unstructured":"Egypt. Am. J. Anim. Vet. Sci. 14, 26\u201332."},{"key":"ref54","unstructured":"http:\/\/www.openveterinaryjournal.com"},{"key":"ref55","doi-asserted-by":"crossref","unstructured":"E. A. Shosha et al.  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Virol."},{"key":"ref65","unstructured":"J. 21(1), 83."},{"key":"ref66","unstructured":"Gethoffer, F., Curland, N., V oigt, U., Woelfing, B.,"},{"key":"ref67","unstructured":"Ludwig, T., Heffels-Redmann, U., Hafez, H.M.,"},{"key":"ref68","unstructured":"Lierz, M. and Siebert, U. 2021. Seroprevalence"},{"key":"ref69","unstructured":"of specific antibodies against avian pathogens in"},{"key":"ref70","unstructured":"free-ranging ring-necked pheasants (Phasianus"},{"key":"ref71","unstructured":"colchicus) in Northwestern Germany. PLos One"},{"key":"ref72","unstructured":"16, e255434."},{"key":"ref73","unstructured":"Ghalyanchilangeroudi, A., Madani, S.A., Najafi, H.,"},{"key":"ref74","unstructured":"Ziafati Kafi, Z., Sadri, N., Sarmadi, S., Eghbali,"},{"key":"ref75","unstructured":"O.O., Jamiri, F., Bakhshi, A.A. and Hosseini,"},{"key":"ref76","unstructured":"H. 2024. The full genome characterization of"},{"key":"ref77","unstructured":"avian encephalomyelitis virus in Iran: a vertical"},{"key":"ref78","unstructured":"transmission case. Virus Genes 60(4), 393\u2013401."},{"key":"ref79","unstructured":"Goto, Y ., Yaegashi, G., Kumagai, Y ., Ogasawara, F.,"},{"key":"ref80","unstructured":"Goto, M. and Mase, M. 2019. Detection of avian"},{"key":"ref81","unstructured":"encephalomyelitis virus in chickens in Japan using"},{"key":"ref82","unstructured":"RT-PCR. J. Vet. Med. Sci. 81, 103\u2013106."},{"key":"ref83","unstructured":"Haryanto, A., Ermawati, R., Wati, V ., Irianingsih, S.H."},{"key":"ref84","unstructured":"and Wijayanti, N. 2016. Analysis of viral protein-2"},{"key":"ref85","unstructured":"encoding gene of avian encephalomyelitis virus"},{"key":"ref86","unstructured":"from field specimens in Central Java region,"},{"key":"ref87","unstructured":"Indonesia. Vet. World. 9, 25\u201331."},{"key":"ref88","unstructured":"Hauck, R., Sent\u00edes-Cu\u00e9, C.G., Wang, Y ., Kern, C.,"},{"key":"ref89","unstructured":"Shivaprasad, H.L., Zhou, H. and Gallardo, R.A."},{"key":"ref90","unstructured":"2017. Evolution of avian encephalomyelitis virus"},{"key":"ref91","unstructured":"during embryo-adaptation. Vet. Microbiol. 204,"},{"key":"ref92","unstructured":"Hoekstra, J. 1964. Experiments with avian"},{"key":"ref93","unstructured":"encephalomyelitis. Br. Vet. J. 120, 322\u2013335."},{"key":"ref94","unstructured":"Kant, A., Balk, F., Born, L., Van Roozelaar, D.,"},{"key":"ref95","unstructured":"Heijmans, J., Gielkens, A. and Ter Huurne, A."},{"key":"ref96","unstructured":"2003. Classification of Dutch and German avian"},{"key":"ref97","unstructured":"reoviruses by sequencing the sigma C protein. Vet."},{"key":"ref98","unstructured":"Res. 34, 203\u2013212."},{"key":"ref99","unstructured":"Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7:"},{"key":"ref100","unstructured":"molecular evolutionary genetics analysis version"},{"key":"ref101","unstructured":"7.0 for bigger datasets. J. Mol. Genet. Evol. 33,"},{"key":"ref102","unstructured":"Lin, W., Lu, P., Li, A., Wu, Y ., Li, H., Chen, F., Ma,"},{"key":"ref103","unstructured":"J. and Xie, Q. 2018. Assessing the efficacy of a"},{"key":"ref104","unstructured":"live vaccine against avian encephalomyelitis virus."},{"key":"ref105","unstructured":"Arch. Virol. 163, 2395\u20132404."},{"key":"ref106","unstructured":"Liu, Q., Yang, Z., Hao, H., Cheng, S., Fan, W., Du,"},{"key":"ref107","unstructured":"E., Xiao, S., Wang, X. and Zhang, S. 2014."},{"key":"ref108","unstructured":"Development of a SYBR Green real-time RT-PCR"},{"key":"ref109","unstructured":"assay for the detection of avian encephalomyelitis"},{"key":"ref110","unstructured":"virus. J. Virol. Methods 206, 46\u201350."},{"key":"ref111","doi-asserted-by":"crossref","unstructured":"Martin, D.P. 2009. Recombination detection and analysis","DOI":"10.1007\/978-1-59745-251-9_9"},{"key":"ref112","unstructured":"using RDP3. Methods. Mol. Biol. 537, 185\u2013205.Martin, D.P., Posada, D., Crandall, K.A. and Williamson,"},{"key":"ref113","unstructured":"C. 2005. A modified bootscan algorithm for"},{"key":"ref114","unstructured":"automated identification of recombinant sequences"},{"key":"ref115","unstructured":"and recombination breakpoints. AIDS Res."},{"key":"ref116","unstructured":"Hum. Retroviruses 21, 98\u2013102; doi:10.1016\/j."},{"key":"ref117","doi-asserted-by":"crossref","unstructured":"asdrhr.2005.09.010","DOI":"10.1088\/1475-7516\/2005\/09\/010"},{"key":"ref118","unstructured":"Mattenberger, F., Latorre, V ., Tirosh, O., Stern, A. and"},{"key":"ref119","unstructured":"Geller, R. 2021. Globally defining the effects of"},{"key":"ref120","unstructured":"mutations in the capsid of a picornavirus. Elife 10,"},{"key":"ref121","unstructured":"Meir, R., Maharat, O., Farnushi, Y . and Simanov, L."},{"key":"ref122","unstructured":"2010. Development of a real-time TaqMan\u00ae RT-"},{"key":"ref123","unstructured":"PCR assay for the detection of infectious bronchitis"},{"key":"ref124","unstructured":"virus in chickens, and comparison of RT-PCR and"},{"key":"ref125","unstructured":"virus isolation. J. Virol. Methods. 163, 190\u2013194."},{"key":"ref126","unstructured":"Mosad, S.M., Rasheed, N., Ali, H.S., El-Khabaz,"},{"key":"ref127","unstructured":"K.A.S., Shosha, E.A.M. and El-Diasty, M. 2021."},{"key":"ref128","unstructured":"Incidence of lumpy skin disease virus with its"},{"key":"ref129","unstructured":"characterization in vaccinated pregnant Holstein"},{"key":"ref130","unstructured":"cows in Dakahlia governorate, Egypt. Ger. J. Vet."},{"key":"ref131","unstructured":"Res. 1(4), 23\u201333."},{"key":"ref132","unstructured":"Pan, J., Narayanan, B., Shah, S., Yoder, J.D., Cifuente,"},{"key":"ref133","unstructured":"J.O., Hafenstein, S. and Bergelson, J.M. 2011."},{"key":"ref134","unstructured":"Single amino acid changes in the virus capsid permit"},{"key":"ref135","unstructured":"coxsackievirus b3 to bind decay-accelerating factor."},{"key":"ref136","unstructured":"J. Virol. 85, 7436\u20137443."},{"key":"ref137","unstructured":"Pohjola, L., Tammiranta, N., Ek-Kommonen, C.,"},{"key":"ref138","unstructured":"Soveri, T., H\u00e4nninen, M.L., Fredriksson Ahomaa,"},{"key":"ref139","unstructured":"M. and Huovilainen, A. 2017. A survey for selected"},{"key":"ref140","unstructured":"avian viral pathogens in backyard chicken farms in"},{"key":"ref141","unstructured":"Finland. Avian Pathol. 46, 166\u2013172."},{"key":"ref142","unstructured":"Quaglia, G., Mescolini, G., Catelli, E., Berto, G.,"},{"key":"ref143","unstructured":"Muccioli, F. and Lupini, C. 2021. Genetic"},{"key":"ref144","unstructured":"heterogeneity among chicken infectious anemia"},{"key":"ref145","unstructured":"viruses detected in Italian fowl. Animals 11(4), 944."},{"key":"ref146","unstructured":"Ramzy, N.M., Ayatollah I Ibrahim., Mohamed S Abd.,"},{"key":"ref147","unstructured":"Eman, E.H. and Shosha. 2024. Isolation and genetic"},{"key":"ref148","unstructured":"diversity of fowlpox virus circulating in chicken"},{"key":"ref149","unstructured":"flocks in Egypt. J. Adv. Vet. Res. 14(5), 799\u2013806."},{"key":"ref150","unstructured":"Raue, R. and Hess, M. 1998. Hexon based PCR\u2019s"},{"key":"ref151","unstructured":"combined with restriction enzyme analysis for rapid"},{"key":"ref152","unstructured":"detection and differentiation of fowl adenoviruses"},{"key":"ref153","unstructured":"and egg drop syndrome virus. J. Virol. Methods 73,"},{"key":"ref154","unstructured":"Sarma, G., Kersting, B.A. and Spina, G. 2019. Field"},{"key":"ref155","unstructured":"safety and efficacy of a unique live virus vaccine for"},{"key":"ref156","unstructured":"controlling avian encephalomyelitis and fowlpox in"},{"key":"ref157","unstructured":"poultry. Vet. World 12, 1291\u20131298."},{"key":"ref158","unstructured":"Sent\u00edes-Cu\u00e9, C.G., Gallardo, R.A., Reimers, N.,"},{"key":"ref159","unstructured":"Bickford, A.A., Charlton, B.R. and Shivaprasad,"},{"key":"ref160","unstructured":"H.L. 2016. Avian encephalomyelitis in Layer"},{"key":"ref161","unstructured":"Pullets Associated with Vaccination. Avian Dis. 60,"},{"key":"ref162","unstructured":"Shafren, D.R. and Tannock, G.A. 1991. Pathogenesis"},{"key":"ref163","unstructured":"of avian encephalomyelitis viruses. J. Gen. Virol."},{"key":"ref164","unstructured":"72(11), 2713\u20132719."},{"key":"ref165","unstructured":"Shosha, E., Abdelnaser, S. and Zanaty, A.M. 2025a. A"},{"key":"ref166","unstructured":"Full-length S1 gene sequencing of a novel emerged"},{"key":"ref167","unstructured":"GI-19 and GI-23 lineages of Infectious bronchitis"},{"key":"ref168","unstructured":"http:\/\/www.openveterinaryjournal.com"},{"key":"ref169","doi-asserted-by":"crossref","unstructured":"E. A. Shosha et al.  Open Veterinary Journal , (2026), V ol. 16(2): 1338-1350","DOI":"10.5455\/OVJ.2026.v16.i2.50"},{"key":"ref170","unstructured":"1350virus currently circulating in chicken flocks in"},{"key":"ref171","unstructured":"Upper Egypt reveals marked genetic diversity and"},{"key":"ref172","unstructured":"recombination events. Virol. J. 22, 135."},{"key":"ref173","unstructured":"Shosha, E.A.E., Abdelnaser, S., Zanaty, A.M.,"},{"key":"ref174","unstructured":"Elzanaty, A.E., Selim, K. and Eldaghayes, I."},{"key":"ref175","unstructured":"2025b. Successive Efficacy Evaluation of Various"},{"key":"ref176","unstructured":"Commercial Live-Attenuated Avian coronavirus"},{"key":"ref177","unstructured":"Vaccination Schedules Against a Local GI-23.3"},{"key":"ref178","unstructured":"Challenge in SPF Broilers. Vaccines 13, 1132."},{"key":"ref179","unstructured":"Shosha, E.A.E., Mohamd, M.K., Shehata, M.A.E.,"},{"key":"ref180","unstructured":"Mohamed, M.H., Eldaghayes, I. and Abdelhafez,"},{"key":"ref181","unstructured":"M.S. 2025. Genomic and evolutionary"},{"key":"ref182","unstructured":"characterization of newly emerged highly"},{"key":"ref183","unstructured":"pathogenic avian influenza H5N1 clade 2.3.4.4b"},{"key":"ref184","unstructured":"viruses circulating in broiler flocks in Upper Egypt"},{"key":"ref185","doi-asserted-by":"crossref","unstructured":"(2023\u20132025). Vet. World 18(12), 3745\u20133760;","DOI":"10.2147\/IDR.S522808"},{"key":"ref186","unstructured":"doi:10.1016\/j.vw.2023"},{"key":"ref187","unstructured":"Shosha, E.A.E.M., Zanaty, A.M., Darwesh, M.M."},{"key":"ref188","unstructured":"and Fotouh, A. 2024. Molecular characterization"},{"key":"ref189","unstructured":"and immunopathological investigation of Avian"},{"key":"ref190","unstructured":"reticuloendotheliosis virus in breeder flocks in"},{"key":"ref191","unstructured":"Egypt. Virol. J. 22(1), 259."},{"key":"ref192","unstructured":"Swayne, D. E., Glisson, J. R., McDougald, L. R.,"},{"key":"ref193","unstructured":"Nolan, L. K., Suarez, D. L., & Nair, V . L. (Eds.)."},{"key":"ref194","unstructured":"Diseases of Poultry, 13th ed. Wiley-Blackwell;"},{"key":"ref195","unstructured":"2013. 1408 pp."},{"key":"ref196","unstructured":"Tamura K Stecher., Peterson, G., Filipski, D. and"},{"key":"ref197","unstructured":"Kumar, S. 2013. Molecular biology and evolution."},{"key":"ref198","unstructured":"Mol. Biol. Evol. 30, 2725\u20132729."},{"key":"ref199","unstructured":"Taunde, P., Timbe, P., Lucas, A.F., Tchamo, C.,"},{"key":"ref200","unstructured":"Chilundo, A., Dos Anjos, F., Costa, R. and"},{"key":"ref201","unstructured":"Bila, C.G. 2017. Serological evidence of avian"},{"key":"ref202","unstructured":"encephalomyelitis virus and Pasteurella multocida"},{"key":"ref203","unstructured":"infections in free-range indigenous chickens in"},{"key":"ref204","unstructured":"Southern Mozambique: a case report. Trop. Anim."},{"key":"ref205","unstructured":"Health Prod. 49, 1047\u20131050."},{"key":"ref206","unstructured":"Temeeyasen, G., Sharafeldin, T., Gharaibeh, S.,"},{"key":"ref207","unstructured":"Sobhy, N.M., Porter, R.E. and Mor, S.K. 2024. A"},{"key":"ref208","unstructured":"New Variant of Avian Encephalomyelitis Virus"},{"key":"ref209","unstructured":"Associated with Neurologic Signs in Turkey Poults."},{"key":"ref210","unstructured":"Pathogens 13(758), 758."},{"key":"ref211","unstructured":"Torre, D.D.L., Nu\u00f1ez, L., Parra, S., Astolfi-Ferreira, C. and"},{"key":"ref212","unstructured":"Ferreira, A. 2018. Detection by rt-pcr and molecular characterization of tremovirus A obtained from clinical"},{"key":"ref213","unstructured":"cases of avian encephalomyelitis (AE) outbreaks in"},{"key":"ref214","unstructured":"brazil. Braz. J. Poultry Sci. 20(3), 527\u2013536."},{"key":"ref215","unstructured":"Varte, L., Deka, D., Gupta, K. and Singh, A. 2023."},{"key":"ref216","unstructured":"Comparative sequence analysis of Meq oncogene"},{"key":"ref217","unstructured":"of Marek\u2019s disease virus field isolates detected in"},{"key":"ref218","unstructured":"Marek\u2019s disease affected birds from vaccinated"},{"key":"ref219","unstructured":"poultry flocks. Indian J. Vet. Pathol. 47, 211\u2013218."},{"key":"ref220","unstructured":"Wang, G., He, Y ., Yan, X., Sun, Y ., Yi, L., Tu, C. and"},{"key":"ref221","unstructured":"He, B. 2023. Virome profiling of chickens with"},{"key":"ref222","unstructured":"hepatomegaly rupture syndrome reveals coinfection"},{"key":"ref223","unstructured":"of multiple viruses. Viruses 15, 1249."},{"key":"ref224","unstructured":"Wei, L., Chee, L.L., Wei, T., Kwang, J., Zhou, J., Wang,"},{"key":"ref225","unstructured":"J., Shi, L. and Liu, J. 2008. The VP1 protein of avian"},{"key":"ref226","unstructured":"encephalomyelitis virus is a major host-protective"},{"key":"ref227","unstructured":"immunogen that serves as diagnostic potential. J."},{"key":"ref228","unstructured":"Virol. Methods 149, 56\u201362."},{"key":"ref229","unstructured":"Wise, M.G., Suarez, D.L., Seal, B.S., Pedersen, J.C.,"},{"key":"ref230","unstructured":"Senne, D.A., King, D.J., Kapczynski, D.R. and"},{"key":"ref231","unstructured":"Spackman, E. 2004. Development of a real-"},{"key":"ref232","unstructured":"time reverse-transcription PCR for detection of"},{"key":"ref233","unstructured":"Newcastle disease virus RNA in clinical samples."},{"key":"ref234","unstructured":"J. Clin. Microbiol. 42, 329\u2013338; doi:10.1016\/j."},{"key":"ref235","unstructured":"jcm.2004.01.016"},{"key":"ref236","unstructured":"Xu, Z., Fan, P., Zhang, C., Guo, M., Bo, Z., Wu, Y . and"},{"key":"ref237","unstructured":"Zhang, X. 2025. Identification of virulence-related"},{"key":"ref238","unstructured":"amino acid mutations of avian encephalomyelitis"},{"key":"ref239","unstructured":"virus associated with vaccination methods. Front."},{"key":"ref240","unstructured":"Vet. Sci. 12, 1548515."},{"key":"ref241","unstructured":"Xue, Q., Guo, H., Feng, Z. and Wang, J. 2016."},{"key":"ref242","unstructured":"Establishment of a real-time RT-PCR assay for"},{"key":"ref243","unstructured":"avian encephalomyelitis virus. Med. Weter. 72,"},{"key":"ref244","unstructured":"Zhang, F., Luo, Y ., Wei, Q., Xiong, L., Xie, Q., Tan,"},{"key":"ref245","unstructured":"J., Wu, C., Li, N. and Kang, Z. 2024. Research"},{"key":"ref246","unstructured":"Note: pathogenetic characteristics of avian"},{"key":"ref247","unstructured":"encephalomyelitis virus in Guangdong and Jiangxi"},{"key":"ref248","unstructured":"Provinces, China. Poult. Sci. 103(2), 103264."},{"key":"ref249","unstructured":"Zhang, G., Li, S., Shen, Z. and Wang, F. 2023. 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