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Single"},{"key":"ref3","unstructured":"Mutations in the VP2 300 Loop Region of the Table 3. Univariable logistic regression: mortality by candidate predictors."},{"key":"ref4","unstructured":"Variable (reference) Category\/ContrastAlive n"},{"key":"ref5","unstructured":"(%)OR95% CI"},{"key":"ref6","unstructured":"lower95% CI"},{"key":"ref7","unstructured":"upperp-value"},{"key":"ref8","unstructured":"Sex (ref: male) Female versus male 10 (23.3) 33 (76.7) 2.29 0.88 5.95 0.087"},{"key":"ref9","unstructured":"Age (ref: \u22653 months) <3 months versus \u22653 months 8 (21.6) 29 (78.4) 2.50 0.90 6.42 0.079"},{"key":"ref10","unstructured":"Breed size (ref: medium\/"},{"key":"ref11","unstructured":"large\/giant)Small (<15 kg) versus"},{"key":"ref12","unstructured":"medium\/large\/giant11 (22.4) 38 (77.6) 2.94 1.10 7.69 0.031"},{"key":"ref13","unstructured":"Vaccination (ref: yes) No versus yes 6 (28.6) 15 (71.4) 1.59 0.51 4.85 0.429"},{"key":"ref14","unstructured":"Symptomatology (ref:"},{"key":"ref15","unstructured":"digestive only)GI + neurological\/respiratory"},{"key":"ref16","unstructured":"versus digestive only21 (29.2) 51 (70.8) 9.71 1.02 92.11 0.048"},{"key":"ref17","unstructured":"Comorbidities (ref: no) Yes versus no 2 (16.7) 10 (83.3) 2.44 0.49 12.15 0.276"},{"key":"ref18","unstructured":"Antigenic variant (ref:"},{"key":"ref19","unstructured":"CPV-2b)CPV-2a versus CPV-2b 2 (16.7) 10 (83.3) 3.15 0.61 16.15 0.169"},{"key":"ref20","unstructured":"CPV-2c versus CPV-2b 4 (23.5) 13 (76.5) 2.05 0.57 7.32 0.271"},{"key":"ref21","unstructured":"Cornell vaccine strain versus"},{"key":"ref22","unstructured":"CPV-2b1 (20.0) 4 (80.0) 2.52 0.26 24.47 0.426"},{"key":"ref23","unstructured":"FPV versus CPV-2b 2 (100) 0 (0) \u2014 \u2014 \u2014 \u2014"},{"key":"ref24","unstructured":"OR = odds ratio; CI = confidence interval; GI = gastrointestinal. 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Isolation"},{"key":"ref40","unstructured":"and immunisation studies of a canine parvo-like"},{"key":"ref41","unstructured":"virus from dogs with haemorrhagic enteritis. Vet."},{"key":"ref42","doi-asserted-by":"crossref","unstructured":"Rec. 105, 156\u2013159; doi:10.1136\/vr.105.8.156","DOI":"10.1136\/vr.105.8.156"},{"key":"ref43","unstructured":"Buonavoglia, C., Martella, V ., Pratelli, A., Tempesta,"},{"key":"ref44","unstructured":"M., Cavalli, A., Buonavoglia, D., Bozzo, G., Elia,"},{"key":"ref45","unstructured":"G., Decaro, N. and Carmichael, L. 2001. Evidence"},{"key":"ref46","unstructured":"for evolution of canine parvovirus type 2 in Italy. J."},{"key":"ref47","unstructured":"Gen. Virol. 82(12), 3021\u20133025; doi:10.1099\/0022-"},{"key":"ref48","unstructured":"1317-82-12-3021"},{"key":"ref49","unstructured":"Calatayud, O., Esper\u00f3n, F., Velarde, R., Oleaga,"},{"key":"ref50","unstructured":"A., Llaneza, L., Ribas, A., Negre, N., Torre,"},{"key":"ref51","unstructured":"A., Rodr\u00edguez, A. and Mill\u00e1n, J. 2020. Genetic"},{"key":"ref52","unstructured":"characterization of Carnivore Parvoviruses in"},{"key":"ref53","unstructured":"Spanish wildlife reveals domestic dog and cat-"},{"key":"ref54","unstructured":"related sequences. Transbound. Emerg. Dis. 67,"},{"key":"ref55","doi-asserted-by":"crossref","unstructured":"626\u2013634; doi:10.1111\/tbed.13378","DOI":"10.1111\/tbed.13378"},{"key":"ref56","unstructured":"Canuti, M., Mira, F., Villan\u00faa, D., Rodr\u00edguez-Pastor,"},{"key":"ref57","unstructured":"R., Guercio, A., Urra, F. and Mill\u00e1n, J. 2025."},{"key":"ref58","unstructured":"Molecular ecology of novel amdoparvoviruses and"},{"key":"ref59","unstructured":"old protoparvoviruses in Spanish wild carnivorans."},{"key":"ref60","unstructured":"Infect. Genet. Evol. 128, 105714; doi:10.1016\/j."},{"key":"ref61","unstructured":"meegid.2025.105714"},{"key":"ref62","unstructured":"Chen, M.R., Guo, X.Y ., Wang, Z.Y ., Jiang, Y .T.,"},{"key":"ref63","unstructured":"Yuan, W.F., Xin, T., Hou, S.H., Song, T.Q., Lin,"},{"key":"ref64","unstructured":"W.D., Zhu, H.F. and Jia, H. 2019. 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Identification of types of canine"},{"key":"ref76","unstructured":"parvovirus circulating in Spain. Vet. Rec. 136, 174\u2013"},{"key":"ref77","doi-asserted-by":"crossref","unstructured":"175; doi:10.1136\/vr.136.7.174","DOI":"10.1136\/vr.136.7.174"},{"key":"ref78","unstructured":"Decaro, N., Desario, C., Addie, D.D., Martella, V ., Vieira,"},{"key":"ref79","unstructured":"M.J., Elia, G., Zicola, A., Davis, C., Thompson, G.,"},{"key":"ref80","unstructured":"Thiry, E., Truyen, U. and Buonavoglia, C. 2007."},{"key":"ref81","unstructured":"The Study Molecular Epidemiology of Canine"},{"key":"ref82","unstructured":"Parvovirus, Europe. Emerg. Infect. 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Evidence for immunisation failure in vaccinated"},{"key":"ref92","unstructured":"adult dogs infected with canine parvovirus type 2c."},{"key":"ref93","unstructured":"New Microbiol. 31, 125\u2013130."},{"key":"ref94","unstructured":"Decaro, N., Martella, V ., Desario, C., Bellacicco,"},{"key":"ref95","unstructured":"A.L., Camero, M., Manna, L., D'Aloja, D. and"},{"key":"ref96","unstructured":"Buonavoglia, C. 2006. First detection of canine"},{"key":"ref97","unstructured":"parvovirus type 2c in pups with haemorrhagic"},{"key":"ref98","unstructured":"enteritis in Spain. J. Vet. Med. B. Infect. Dis. Vet."},{"key":"ref99","unstructured":"Public Health 53, 468\u2013472; doi:10.1111\/j.1439-"},{"key":"ref100","unstructured":"0450.2006.00974.x"},{"key":"ref101","unstructured":"Ford, J., Mcendaffer, L., Renshaw, R., Molesan,"},{"key":"ref102","unstructured":"A. and Kelly, K. 2017. Parvovirus Infection Is"},{"key":"ref103","unstructured":"Associated With Myocarditis and Myocardial"},{"key":"ref104","unstructured":"Fibrosis in Young Dogs. Vet. Pathol. 54, 964\u2013971;"},{"key":"ref105","doi-asserted-by":"crossref","unstructured":"doi:10.1177\/0300985817725387","DOI":"10.1177\/0300985817725387"},{"key":"ref106","unstructured":"Kwan, E., Carrai, M., Lanave, G., Hill, J., Parry, K.,"},{"key":"ref107","unstructured":"Kelman, M., Meers, J., Decaro, N., Beatty, J.A.,"},{"key":"ref108","unstructured":"Martella, V . and Barrs, V .R. 2021. Analysis of"},{"key":"ref109","unstructured":"canine parvoviruses circulating in Australia reveals"},{"key":"ref110","unstructured":"predominance of variant 2b and identifies feline"},{"key":"ref111","unstructured":"parvovirus-like mutations in the capsid proteins."},{"key":"ref112","unstructured":"Transbound. Emerg. 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Pract."},{"key":"ref119","doi-asserted-by":"crossref","unstructured":"50, 1307\u20131325; doi:10.1016\/j.cvsm.2020.07.008","DOI":"10.1016\/j.cvsm.2020.07.008"},{"key":"ref120","unstructured":"Mukhopadhyay, H.K., Matta, S.L., Amsaveni, S.,"},{"key":"ref121","unstructured":"Antony, P.X., Thanislass, J. and Pillai, R.M. 2014."},{"key":"ref122","unstructured":"Phylogenetic analysis of canine parvovirus partial"},{"key":"ref123","unstructured":"VP2 gene in India. Virus. Genes. 48, 89\u201395;"},{"key":"ref124","doi-asserted-by":"crossref","unstructured":"doi:10.1007\/s11262-013-1000-5","DOI":"10.1007\/s11262-013-1000-5"},{"key":"ref125","unstructured":"Ohshima, T., Hisaka, M., Kawakami, K., Kishi, M.,"},{"key":"ref126","unstructured":"Tohya, Y . and Mochizuki, M. 2008. Chronological"},{"key":"ref127","unstructured":"analysis of canine parvovirus type 2 isolates in"},{"key":"ref128","unstructured":"Japan. J. Vet. Med. Sci. 70, 769\u2013775; doi:10.1292\/"},{"key":"ref129","unstructured":"jvms.70.769"},{"key":"ref130","unstructured":"Packianathan, R., Hodge, A., Wright, J., Lavidis,"},{"key":"ref131","unstructured":"L., Ameiss, K., Yip, H.Y .E., Akbarzadeh, M.,"},{"key":"ref132","unstructured":"Sharifian, M., Amanollahi, R., Khabiri, A. and"},{"key":"ref133","unstructured":"Hemmatzadeh, F. 2022. Cross-Neutralization of"},{"key":"ref134","unstructured":"Vanguard C4 Vaccine Against Australian Isolates of"},{"key":"ref135","unstructured":"Canine Parvovirus Variants CPV-2a, CPV-2b, and"},{"key":"ref136","unstructured":"CPV-2c. Viral Immunol. 35, 553\u2013558; doi:10.1089\/"},{"key":"ref137","unstructured":"vim.2022.0027"},{"key":"ref138","unstructured":"Parrish, C.R. 1999. Host range relationships and the"},{"key":"ref139","unstructured":"evolution of canine parvovirus. Vet. 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