{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:42:30Z","timestamp":1787510550196,"version":"build-2736575974"},"reference-count":0,"publisher":"Sociedad Argentina de Investigacion Odontologica","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Odontol Latinoam"],"abstract":"<jats:p>The aim of this study is to compare the efficacy of two methods for collecting saliva samples from infants  under 2 years of age for cariogenic streptococci (CS) count. Two collection methods were applied in 11  infants. In Method (A), saliva samples were collected by swabbing the inner cheek mucosa and floor of  the mouth in figure of eight motions with a sterile cotton swab until it was soaked. In method (B), saliva  samples were collected by aspiration of 1 ml of saliva with a sterile plastic syringe on the floor of the  mouth, after stimulation with glove. The samples were cultured in modified Gold\u2019s broth (MSMG), and  on trypticase, yeast extract, sucrose, cystine and bacitracin culture medium (TYSCB). In method (A),  the swab with the sample was unloaded in situ on TYSCB and placed in PBS medium for transport.  Then, 100 \u03bcl of the eluate was seeded in MSMG. In method (B) 100 \u03bcl were seeded in TYSCB and 100  \u03bcl in MSMG. Both culture media were incubated under capnophilic conditions for 48 hours at 37 \u00b0C.  Colony forming units (CFU\/ml) were counted by calibrated operators (kappa = 0.75). The presence of  cariogenic streptococci (CS) (Streptococcus mutans-Streptococcus sobrinus) was determined by qPCR  in the samples collected by both methods. The CFU\/ml counts in MSMG differed significantly between  methods (p = 0.021). In TYSCB, the recovery of CFU\/ml was higher in method (A), without significant  difference (p = 0.705). The molecular technique detected presence of CS, with no difference between  collection methods. Collecting saliva samples by swabbing proved more effective in terms of recovery of microorganisms,  and did not affect the detection of presence of CS by molecular techniques.<\/jats:p>","DOI":"10.54589\/aol.35\/1\/51","type":"journal-article","created":{"date-parts":[[2022,5,28]],"date-time":"2022-05-28T10:05:41Z","timestamp":1653732341000},"page":"51-57","source":"Crossref","is-referenced-by-count":9,"title":["Saliva sampling methods. Cariogenic streptococci count using two  different methods of saliva collection in children"],"prefix":"10.54589","volume":"35","author":[{"given":"Celina","family":"Cornejo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Salgado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Molgatini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Gliosca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo","family":"Squassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"31696","published-online":{"date-parts":[[2022,4,29]]},"container-title":["Acta Odontol\u00f3gica Latinoamericana"],"original-title":[],"deposited":{"date-parts":[[2022,5,28]],"date-time":"2022-05-28T10:05:52Z","timestamp":1653732352000},"score":1,"resource":{"primary":{"URL":"https:\/\/actaodontologicalat.com\/wp-content\/uploads\/2022\/05\/aol_2022_35-1-51.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,29]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,4,29]]}},"URL":"https:\/\/doi.org\/10.54589\/aol.35\/1\/51","relation":{},"ISSN":["1852-4834"],"issn-type":[{"value":"1852-4834","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,29]]}}}