{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:20:35Z","timestamp":1761765635580},"reference-count":0,"publisher":"Canadian Urological Association Journal","issue":"5-6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CUAJ"],"abstract":"<jats:p>Background: Aquaporins (AQPs) have recently been reported to be expressed in rat and human urothelium. Nitric oxide (NO) is thought to play a role in the bladder overactivity related to bladder outlet obstruction (BOO). The purpose of this study is to investigate the effect of BOO on the expression of AQP2-3 and nitric oxide synthase (NOS) isoforms in rat urothelium.Methods: Female Sprague-Dawley rats (230-240 g, n = 60) were divided into 2 groups. The control group (n = 30) and the partial bladder outlet obstruction (BOO) group (n = 30). After 4 weeks, we performed a urodynamic study to measure the contraction interval and contraction pressure. The expression and cellular localization of AQP2-3, endothelial nitric oxide synthase (eNOS) and neuronalnitric oxide synthase (nNOS) were determined by Western blot and immunohistochemistry.Results: On the cystometrogram, the estimated contraction interval time (minutes, mean \u00b1 SE) was significantly lower in the BOO group (3.0 \u00b1 0.9) than in the control group (6.3 \u00b1 0.4; p &lt; 0.05). AQP2 was localized in the cytoplasm of the epithelium, whereas AQP3 was found only in the cell membrane of the epithelium. The protein expression of AQP2-3, eNOS and nNOS was significantly increased in the BOO group.Conclusion: Detrusor overactivity induced by BOO causes a significant increase in the expression of AQP2-3, eNOS, and nNOSin rat urinary bladder. This may imply that the AQPs and NOS isoforms have a functional role in the bladder dysfunction that occurs in association with BOO.<\/jats:p>","DOI":"10.5489\/cuaj.993","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T09:46:39Z","timestamp":1368524799000},"page":"268","source":"Crossref","is-referenced-by-count":21,"title":["Effect of detrusor overactivity on the expression of aquaporins and nitric oxide synthase in rat urinary bladder following bladder outlet obstruction"],"prefix":"10.5489","volume":"7","author":[{"given":"Sun-Ouck","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongjune","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung Hee","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyu Youn","family":"Ahn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongdeuk","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwangsung","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo Bang","family":"Ryu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3456","published-online":{"date-parts":[[2013,5,13]]},"container-title":["Canadian Urological Association Journal"],"original-title":[],"link":[{"URL":"http:\/\/cuaj.ca\/index.php\/journal\/article\/viewFile\/993\/1029","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/cuaj.ca\/index.php\/journal\/article\/viewFile\/993\/1029","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2016,10,11]],"date-time":"2016-10-11T07:46:39Z","timestamp":1476171999000},"score":1,"resource":{"primary":{"URL":"http:\/\/cuaj.ca\/index.php\/journal\/article\/view\/993"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,13]]},"references-count":0,"journal-issue":{"issue":"5-6","published-online":{"date-parts":[[2013,5,13]]}},"URL":"https:\/\/doi.org\/10.5489\/cuaj.993","relation":{},"ISSN":["1920-1214"],"issn-type":[{"value":"1920-1214","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,13]]}}}