{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,9]],"date-time":"2026-11-09T16:49:31Z","timestamp":1794242971441,"version":"4.9.0"},"reference-count":23,"publisher":"Baishideng Publishing Group Inc.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World J Exp Med"],"abstract":"BACKGROUND<\/jats:p>\n Acute myeloid leukemia (AML) is a complicated disease with uncontrolled hematopoietic precursor proliferation induced by various genetic alterations. Runt-related transcription factor-1 (RUNX1 ) is commonly disrupted by chromosomal translocations in hematological malignancies.<\/jats:p>\n AIM<\/jats:p>\n To characterize RUNX1 gene rearrangements and copy number variations in newly diagnosed adult AML patients, with an emphasis on the impact of clinical and laboratory features on the outcome.<\/jats:p>\n METHODS<\/jats:p>\n Fluorescence in situ hybridization was used to test RUNX1 gene alterations in 77 newly diagnosed adult AML cases. NPM1 , FLT3\/ITD , FLT3\/TKD , and KIT mutations were tested by PCR. Prognostic clinical and laboratory findings were studied in relation to RUNX1 alterations.<\/jats:p>\n RESULTS<\/jats:p>\n RUNX1 abnormalities were detected by fluorescence in situ hybridization in 41.6% of patients: 20.8% had translocations, 22.1% had amplification, and 5.2% had deletion. Translocations prevailed in AML-M2 (P = 0.019) with a positive expression of myeloperoxidase (P = 0.031), whereas deletions dominated in M4 and M5 subtypes (P = 0.008) with a positive association with CD64 expression (P = 0.05). The modal chromosomal number was higher in cases having amplifications (P = 0.007) and lower in those with deletions (P = 0.008). RUNX1 abnormalities were associated with complex karyotypes (P < 0.001) and were mutually exclusive of NPM1 mutations. After 44 months of follow-up, RUNX1 abnormalities affected neither patients\u2019 response to treatment nor overall survival.<\/jats:p>\n CONCLUSION<\/jats:p>\n RUNX1 abnormalities were mutually exclusive of NPM1 mutations. RUNX1 abnormalities affected neither patients\u2019 response to treatment nor overall survival.<\/jats:p>","DOI":"10.5493\/wjem.v15.i1.99516","type":"journal-article","created":{"date-parts":[[2024,12,25]],"date-time":"2024-12-25T05:58:41Z","timestamp":1735106321000},"source":"Crossref","is-referenced-by-count":3,"title":["Prevalence of RUNX1<\/i> gene alterations in de novo<\/i> adult acute myeloid leukemia"],"prefix":"10.5493","volume":"15","author":[{"given":"Hoda M","family":"Abd El-Ghany","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona S","family":"El Ashry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona S","family":"Abdellateif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Rabea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nada","family":"Sultan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omnia Y","family":"Abd El Dayem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2060","published-online":{"date-parts":[[2025,3,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21551"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.194258"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1242\/dev.148296"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27841"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.15406\/icpjl.2017.05.00123"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1182\/blood.v124.21.123.123"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0196181"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.n2026"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.5045\/br.2020.S001"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-08-733196"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-99-115518"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1159\/isbn.978-3-318-06861-0"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401592"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400272101"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-11-814442"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-05-223784"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V120.21.2517.2517"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1186\/s43042-021-00179-4"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22177"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.25240"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.lrr.2023.100385"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergen.2019.07.006"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1080\/16078454.2020.1858610"}],"container-title":["World Journal of Experimental Medicine"],"original-title":[],"link":[{"URL":"https:\/\/www.wjgnet.com\/2220-315X\/full\/v15\/i1\/99516.htm","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.wjgnet.com\/2220-315X\/full\/v15\/i1\/99516.htm","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,25]],"date-time":"2024-12-25T06:21:52Z","timestamp":1735107712000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.wjgnet.com\/2220-315X\/full\/v15\/i1\/99516.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,20]]},"references-count":23,"journal-issue":{"issue":"1"},"alternative-id":["99516"],"URL":"https:\/\/doi.org\/10.5493\/wjem.v15.i1.99516","relation":{},"ISSN":["2220-315X"],"issn-type":[{"value":"2220-315X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3,20]]}}}