{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T02:22:22Z","timestamp":1771294942847,"version":"3.50.1"},"reference-count":16,"publisher":"Baishideng Publishing Group Inc.","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World J Transplant"],"abstract":"BACKGROUND<\/jats:p>\n Marginal donation after circulatory death (DCD) liver grafts are carefully used to combat the constant shortage of donors. Clinically, the worst outcomes are mainly related to severe ischemia-reperfusion-injury and the dangerous effect of various inflammatory cytokines (CK). The machine perfusion (MP) is a promising device to rescue these grafts.<\/jats:p>\n AIM<\/jats:p>\n To analyze the role of MP connected to a sorbent cartridge (PerSorb\u00ae) and used for very damaged DCD pig livers.<\/jats:p>\n METHODS<\/jats:p>\n Seven grafts were procured from pigs from a slaughterhouse. Grafts were made very marginal with at least 60 minutes of donor warm ischemia time and 24 hours of static-cold ischemia time: (1) 3 grafts were perfused in hypothermic MP with PerSorb (Sorb); (2) 2 other grafts in hypothermic MP (HMP) without the cartridge (NoSorb); and (3) The other 2 livers stored in the ice box (NoTreat). The CK were measured at HMP start (T0) and at the end (Tend). Biopsies were taken at T0 and Tend.<\/jats:p>\n RESULTS<\/jats:p>\n All 5 grafts treated with HMP had a negative lactate trend after 3 hours of treatment (8.83 at T0 vs 6.4 at Tend of Sorb; 15 at T0 vs 5.45 at Tend for NoSorb, P value > 0.05). At Tend, both Sorb and NoSorb groups had better hemodynamic parameters, comparable between the two groups. Enzyme-linked immunosorbent assay analysis showed a reduction of monocyte chemotactic protein-1, tumor necrosis factor-alpha and interleukin-1\u03b2 for NoSorb group at Tend and a complete downregulation to physiological levels of the same CK in Sorb livers after 3 hours of treatment. Biopsies showed a reduction of the perisinusoidal edema for the Sorb grafts compared with the NoSorb livers.<\/jats:p>\n CONCLUSION<\/jats:p>\n These data suggest a potential protective role of treatment of grafts with MP and sorbent cartridge in reducing the inflammatory response after a severe ischemic injury.<\/jats:p>","DOI":"10.5500\/wjt.v15.i2.99287","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:40:41Z","timestamp":1740098441000},"source":"Crossref","is-referenced-by-count":3,"title":["Haemoadsorption cartridge connected to the machine perfusion for donation after circulatory death porcine liver marginal grafts"],"prefix":"10.5500","volume":"15","author":[{"given":"Irene","family":"Scalera","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rossana","family":"Franzin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Stasi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonino","family":"Castellaneta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrico","family":"Fischetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Morelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margherita","family":"Raele","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio","family":"Panetta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andjela","family":"Kurevija","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Pulga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Atti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loreto","family":"Gesualdo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2060","published-online":{"date-parts":[[2025,6,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.16219"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/liv.14091"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00631"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2021.155705"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1002\/lt.25817"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1159\/000500013"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-017-1314-5"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-62979-9"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.14573\/altex.1909131"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1111\/aor.14351"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26129"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2012.225"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.3727\/105221617X15042750874156"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1002\/cld.847"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3390\/cells8101131"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.788935"}],"container-title":["World Journal of Transplantation"],"original-title":[],"link":[{"URL":"https:\/\/www.wjgnet.com\/2220-3230\/full\/v15\/i2\/99287.htm","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.wjgnet.com\/2220-3230\/full\/v15\/i2\/99287.htm","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:41:22Z","timestamp":1740098482000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.wjgnet.com\/2220-3230\/full\/v15\/i2\/99287.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,18]]},"references-count":16,"journal-issue":{"issue":"2"},"alternative-id":["99287"],"URL":"https:\/\/doi.org\/10.5500\/wjt.v15.i2.99287","relation":{},"ISSN":["2220-3230"],"issn-type":[{"value":"2220-3230","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6,18]]}}}