{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:05:22Z","timestamp":1773389122199,"version":"3.50.1"},"reference-count":26,"publisher":"Baishideng Publishing Group Inc.","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["World J Virol"],"abstract":"<jats:p>BACKGROUND<\/jats:p>\n                  <jats:p>Klebsiella  species are important opportunistic pathogens responsible for various hospital- and community-acquired infections. The rise of multidrug-resistant strains, especially those producing extended-spectrum beta-lactamases (ESBL) and AmpC beta-lactamases, poses a major challenge to effective antimicrobial therapy and infection control.<\/jats:p>\n                  <jats:p>AIM<\/jats:p>\n                  <jats:p>To determine the antimicrobial susceptibility pattern of Klebsiella  isolates and to detect the presence of ESBL and AmpC beta-lactamases.<\/jats:p>\n                  <jats:p>METHODS<\/jats:p>\n                  <jats:p>A one-year cross-sectional study was conducted on 920 Gram-negative isolates, from which 130 non-repetitive Klebsiella  isolates (14.13%) were selected. Antimicrobial susceptibility testing was performed, and ESBL and AmpC production were identified using standard phenotypic confirmatory methods.<\/jats:p>\n                  <jats:p>RESULTS<\/jats:p>\n                  <jats:p>Among 130 Klebsiella  isolates, Klebsiella pneumoniae  was the predominant species (n  = 92, 70.76%). Most isolates were obtained from patients aged 45-60 years (n  = 33, 25.38%) and from pus samples (n  = 55, 42.3%), with the highest frequency from surgical departments (n  = 36, 27.7%). Among the tested antibiotics, gentamicin showed the greatest susceptibility (n  = 81, 62.3%). ESBL production was detected in 77 isolates (59.2%), while 62 isolates (47.69%) produced AmpC beta-lactamases. Co-production of ESBL and AmpC was found in 35 isolates (26.92%).<\/jats:p>\n                  <jats:p>CONCLUSION<\/jats:p>\n                  <jats:p>Klebsiella  species are significant nosocomial and opportunistic pathogens characterized by high multidrug resistance, often through the production of extended-spectrum and AmpC beta-lactamases. Surveillance, confirmatory testing, and infection control guide antibiotic use and prevent spread.<\/jats:p>","DOI":"10.5501\/wjv.v15.i1.118602","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T05:09:46Z","timestamp":1773378586000},"source":"Crossref","is-referenced-by-count":0,"title":["Study of extended-spectrum beta-lactamases and AmpC beta-lactamases producing\n                    <i>Klebsiella<\/i>\n                    from clinical samples in tertiary care hospital of Punjab"],"prefix":"10.5501","volume":"15","author":[{"given":"Harpreet","family":"Kaur","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shilpa","family":"Arora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishal","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirandeep","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shivani","family":"Kamboj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2060","published-online":{"date-parts":[[2026,3,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2022.103998"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-30746-X_8"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.38860"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/s0255-0857(21)02884-x"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4846159"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.18203\/2320-6012.ijrms20181043"},{"key":"B7","unstructured":"Gupta V, Kumarasamy K, Gulati N, Garg R, Krishnan P, Chander J. 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J Pure Appl Microbiol 2014; 4099-4105"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.7860\/JCDR\/2012\/5016.2673"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.15171\/ijep.2017.20"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.18203\/2320-6012.ijrms20150817"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.22207\/jpam.16.4.36"},{"key":"B17","doi-asserted-by":"crossref","unstructured":"Hymavathi R, Kumari SS, Swarnalatha G, Surekha A. Prevalence of ESBL-producing Klebsiella species isolated from respiratory samples at intensive care units. Ann Int Med Dent Res 2018; MB6-MB10","DOI":"10.21276\/aimdr.2018.4.4.MB2"},{"key":"B18","unstructured":"Biradar S, Roopa C. Isolation and Antibiogram of Klebsiella species from Various Clinical Specimens. 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