{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T05:29:39Z","timestamp":1737005379079,"version":"3.33.0"},"reference-count":0,"publisher":"Egyptian Association of Immunologists","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["EJI"],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:p>Platelets are hyperactive in patients with type2 diabetes (T2DM), they adhere to vascular endothelium and play a key role in macrovascular complications. Platelets activity can be measured by flow-cytometry (cluster of differentiation (CD) 41, CD 42, CD 62, CD 63), which allows detection of surface antigens in a sensitive and specific manner. This study aimed to describe platelets activity in T2DM in association with cardiovascular and cerebrovascular complications in relation to duration of diabetes (DM). This was a case-control study with 130 participants (65 diabetic cases and 65 normal controls). All cases were subjected to history and clinical examination, base-line laboratory investigations and surface expression of platelets receptors e.g. CD 41% and mean fluorescent intensity (MFI), CD 42% and MFI, CD 62% and MFI, CD 63% and MFI were determined by flow-cytometry. There was a statistically significant higher expression of CD 62%, CD 62 MFI, CD 63% and CD 63 MFI (p&lt;0.001 for all) in diabetic cases compared to controls. There were significantly higher CD 62 %, CD 62 MFI, CD 63% and CD 63 MFI in cases with cardiovascular complications (p=0.001, p&lt;0.001, p=0.05 and p=0.007, respectively) and in cases with cerebrovascular complications compared to cases without complications (p=0.05, p=0.008, p=0.035, p=0.017, respectively). A significant positive correlation was found between glycated hemoglobin, body mass index and CD 62 %, CD 62 MFI and CD 63%. Using the receiver operating characteristic curve showed that CD 62 %, CD 62 MFI, CD 63 % and CD 63 MFI have a diagnostic ability to early predict DM (area under the curve (AUC)=0.998) as well as cardiovascular (AUC=0.855) and cerebrovascular (AUC=0.765) complications. In conclusion CD 62%, CD 62 MFI, CD 63% and CD 63 MFI markers have a diagnostic ability for early prediction of cardiovascular and cerebrovascular complications among diabetic patients.<\/jats:p>","DOI":"10.55133\/eji.320101","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T13:38:47Z","timestamp":1736948327000},"page":"01-15","source":"Crossref","is-referenced-by-count":0,"title":["Using flow-cytometry in measuring platelet activity in type 2 diabetes and predicting macrovascular complications"],"prefix":"10.55133","volume":"32","author":[{"name":"Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eman","family":"Badawy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"33636","published-online":{"date-parts":[[2025,1,1]]},"container-title":["Egyptian journal of Immunology"],"original-title":[],"deposited":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T13:38:49Z","timestamp":1736948329000},"score":1,"resource":{"primary":{"URL":"https:\/\/ejimmunology.org\/vol-32-1-2025-01\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1,1]]},"published-print":{"date-parts":[[2025,1,1]]}},"URL":"https:\/\/doi.org\/10.55133\/eji.320101","relation":{},"ISSN":["1110-4902","2090-2506"],"issn-type":[{"value":"1110-4902","type":"print"},{"value":"2090-2506","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,1]]}}}