{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T15:21:26Z","timestamp":1780500086595,"version":"3.54.1"},"reference-count":0,"publisher":"Egyptian Association of Immunologists","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["EJI"],"published-print":{"date-parts":[[2026,4,1]]},"abstract":"<jats:p>Regulatory B cells (Bregs) maintain immune homeostasis through anti-inflammatory cytokine production, yet their role in systemic lupus erythematosus (SLE) remains poorly understood. This study evaluated concentrations of CD19+CD24 high CD27+ regulatory B cell frequencies and serum interleukin-10 (IL-10) and interleukin-35 (IL-35) in 40 SLE patients compared to 25 normal controls. Patients in this cross-sectional observational study were stratified by SLE Disease Activity Index (SLEDAI) scores into Group I: SLE patients with active disease (\u22655) and Group II: SLE patients with inactive disease (\u22644) disease groups. CD19+CD24highCD27+ B cells were quantified by flow cytometry, while serum IL-10 and IL-35 levels by enzyme-linked immunosorbent assays. Active SLE patients demonstrated significantly reduced CD19+CD24 highCD27+ B cells (23.20\u00d710\u2076) compared to controls (56.40\u00d710\u2076, p&lt;0.001). IL-35 levels were markedly decreased in active disease patients (54.04\u00b18.10 pg\/ml) versus patients with inactive disease (83.38\u00b120.05 pg\/ml) and controls (89.04\u00b110.86 pg\/ml, p&lt;0.001). Conversely, IL-10 concentrations were elevated in SLE active patients (4.44\u00b11.27 pg\/ml) compared to inactive patients and controls (1.95\u00b10.56 and 2.03\u00b10.61 pg\/ml, respectively, p&lt;0.001). Strong correlations were observed between regulatory B cell counts and SLEDAI scores (r=-0.547, p&lt;0.001). In conclusion, active SLE is characterized by impaired regulatory B cell populations and dysregulated cytokine profiles, with reduced IL-35 and elevated IL-10 levels, correlated with disease activity.<\/jats:p>","DOI":"10.55133\/eji.330204","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T11:04:57Z","timestamp":1776164697000},"page":"33-42","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of CD19+ CD24+CD27 B cell and serum level of interleukin 10 and interleukin 35 in patients with systemic lupus erythematosus"],"prefix":"10.55133","volume":"33","author":[{"given":"Nashwa","family":"Abdeen","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology, Faculty of Medicine, Tanta University, Tanta, Egypt."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"33636","published-online":{"date-parts":[[2026,4,1]]},"container-title":["The Egyptian journal of Immunology"],"original-title":[],"deposited":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T11:04:57Z","timestamp":1776164697000},"score":1,"resource":{"primary":{"URL":"https:\/\/ejimmunology.org\/vol-33-2-2026-04"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,4,1]]},"published-print":{"date-parts":[[2026,4,1]]}},"URL":"https:\/\/doi.org\/10.55133\/eji.330204","relation":{},"ISSN":["1110-4902","2090-2506"],"issn-type":[{"value":"1110-4902","type":"print"},{"value":"2090-2506","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,1]]}}}