{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T20:00:29Z","timestamp":1771012829039,"version":"3.50.1"},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"7-8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,8,1]]},"abstract":"<jats:p>As an extension of our studies on the antitumour properties of various hemocyanins, we sought to compare the antiproliferative effects of hemocyanins derived from two snail species: Helix lucorum (HlH) and Helix aspersa (HaH). This is the first report on the antitumour effects of HaH. We hypothesized that HaH has antitumour effects not only against bladder cancer, as previously shown with other hemocyanins, but also on other cancer cell lines. The antiproliferative properties of the mentioned hemocyanins were investigated in vitro on the following human cell lines: bladder cancer (CAL-29 and T-24), ovarian cancer (FraW\u00fc), acute monocytic leukemia (THP-1), prostate cancer (DU-145), glioma cancer (LN-18), and Burkitt\u2019s lymphoma (Daudi). The properties of HaH were compared to those of HlH, keyhole limpet hemocyanin (KLH), and two positive controls (doxorubicin and mitomycin C). An antiproliferative effect of the total molecule and one structural subunit of HaH, bc-HaH, against both bladder cancer cell lines, T-24 and CAL-29, was observed. The cytotoxic effect of HaH ranged between 15% and 60% among the other tested cell lines. The endotoxin contamination did not affect the efficacy of HaH. Therefore, HlH and HaH could be appropriate for more detailed investigations of their use as antitumour agents for the studied cancers.<\/jats:p>","DOI":"10.5560\/znc.2013-0148","type":"journal-article","created":{"date-parts":[[2014,8,6]],"date-time":"2014-08-06T14:44:24Z","timestamp":1407336264000},"page":"325-334","source":"Crossref","is-referenced-by-count":22,"title":["In vitro Antiproliferative Effect of Helix aspersa Hemocyanin on Multiple Malignant Cell Lines"],"prefix":"10.1515","volume":"69","author":[{"given":"Olga","family":"Antonova","sequence":"first","affiliation":[{"name":"Department of Medical Genetics, Medical University-Sofia, 2 Zdrave St., Sofia 1431, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Draga","family":"Toncheva","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Medical University-Sofia, 2 Zdrave St., Sofia 1431, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Georg","family":"Rammensee","sequence":"additional","affiliation":[{"name":"Institute for Cell Biology, Department of Immunology, University of T\u00fcbingen, Auf der Morgenstelle 15, D-72076 T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Floetenmeyer","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Developmental Biology, Spemannstra\u00dfe 35, D-72076 T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Stevanovic","sequence":"additional","affiliation":[{"name":"Institute for Cell Biology, Department of Immunology, University of T\u00fcbingen, Auf der Morgenstelle 15, D-72076 T\u00fcbingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavlina","family":"Dolashka","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 9 G. Bonchev St., Sofia 1113, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2014,6,2]]},"container-title":["Zeitschrift f\u00fcr Naturforschung C"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.degruyter.com\/view\/j\/znc.2014.69.issue-7-8\/znc.2013-0148\/znc.2013-0148.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.5560\/znc.2013-0148\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.5560\/znc.2013-0148\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T16:30:48Z","timestamp":1624379448000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.5560\/znc.2013-0148\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,2]]},"references-count":0,"journal-issue":{"issue":"7-8","published-online":{"date-parts":[[2014,6,2]]},"published-print":{"date-parts":[[2014,8,1]]}},"alternative-id":["10.5560\/znc.2013-0148"],"URL":"https:\/\/doi.org\/10.5560\/znc.2013-0148","relation":{},"ISSN":["1865-7125","0939-5075"],"issn-type":[{"value":"1865-7125","type":"electronic"},{"value":"0939-5075","type":"print"}],"subject":[],"published":{"date-parts":[[2014,6,2]]}}}