{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:38:00Z","timestamp":1792683480336,"version":"4.5.0"},"reference-count":0,"publisher":"The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) - DIGITAL COMMONS JOURNALS","issue":"4","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Turkish Journal of Medical Sciences"],"abstract":"<jats:p>Background\/aim: The therapeutic effects of hydrolyzed silk fibroin derived from Bombyx hatayensis and a commercially available ozonized gel, administered individually and in combination, were evaluated in a rat model of corneal alkali burn.\nMaterials and methods: A standardized corneal alkali burn was induced in the right eyes of Wistar albino rats using 1 N sodium hydroxide (NaOH). Animals were randomly assigned to six groups: negative control, positive control (PC), hydrolyzed silk fibroin (SF), ozonized gel (O), combined treatment (SF + O), and antibiotic treatment. Topical treatments were administered for 21 consecutive days. Clinical evaluation included assessments of corneal opacity, neovascularization, and epithelial healing. On day 21, corneal tissues were harvested for biochemical analysis of TGF-\u03b2, VEGF, and TNF-\u03b1 levels, as well as for histopathological assessment.\nResults: Significantly elevated levels of TGF-\u03b2, VEGF, and TNF-\u03b1 were observed in untreated alkali-burned corneas compared with controls (p &lt; 0.001). All treatment groups demonstrated significant reductions in these mediators compared with the PC group (p &lt; 0.001). The SF and O groups showed comparable effects, whereas the SF + O group exhibited the lowest mean VEGF and TNF-\u03b1 levels among the treatment groups, with no statistically significant difference compared with the antibiotic group (p &gt; 0.05). Histopathological findings were consistent with the biochemical results, demonstrating improved epithelial integrity, reduced inflammatory cell infiltration, and more organized stromal architecture, particularly in the SF + O group.\nConclusion: Hydrolyzed silk fibroin derived from Bombyx hatayensis, particularly when combined with ozonized gel, effectively attenuated inflammatory and angiogenic responses following corneal alkali injury. Outcomes comparable to those of conventional topical management were achieved with the combined therapy, with no statistically significant differences in biochemical and structural parameters, supporting its potential as a biocompatible, cell-free adjunctive strategy for severe chemical corneal burns.<\/jats:p>","DOI":"10.55730\/1300-0144.6372","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:25:09Z","timestamp":1787066709000},"page":"1274-1286","source":"Crossref","is-referenced-by-count":0,"title":["Can hydrolyzed silk fibroin derived from Bombyx hatayensis and ozonized gel modulate inflammatory and angiogenic responses in a corneal alkali burn model? \u200b\u200b"],"prefix":"10.55730","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5750-6825","authenticated-orcid":true,"given":"\u015eULE","family":"BARMAN KAK\u0130L","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HAMDULLAH SUPH\u0130","family":"BAYRAKTAR","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5315-150X","authenticated-orcid":true,"given":"OKAN","family":"SOYLU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0590-6405","authenticated-orcid":true,"given":"\u0130SHAK","family":"G\u00d6K\u00c7EK","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MERYEM \u0130LKAY","family":"KARAG\u00dcL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3152-3069","authenticated-orcid":true,"given":"ZEHRA","family":"DEM\u0130RAY ASO\u011eLU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"34691","published-online":{"date-parts":[[2026,8,13]]},"container-title":["Turkish Journal of Medical Sciences"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:41:36Z","timestamp":1792680096000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.tubitak.gov.tr\/medical\/vol56\/iss4\/38"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,13]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,8,13]]}},"URL":"https:\/\/doi.org\/10.55730\/1300-0144.6372","relation":{},"ISSN":["1300-0144"],"issn-type":[{"value":"1300-0144","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,13]]}}}