{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:40:43Z","timestamp":1792590043216,"version":"4.5.0"},"reference-count":0,"publisher":"The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) - DIGITAL COMMONS JOURNALS","issue":"3","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Turkish Journal of Chemistry"],"abstract":"<jats:p>AFP, a cancer biomarker, is monitored during fetal development and in the serum of pregnant women. Sensitive and rapid monitoring of AFP is crucial for effective management. In this study, screen-printed carbon electrodes (SPCEs) modified with multi-walled carbon nanotubes (MWCNT-COOH), polyAzur A (PAA), which was prepared in a deep eutectic solution (DES), and gold nanoparticles (AuNP) were fabricated. AFP biosensors were developed for practical, sensitive, low-cost, and rapid detection of AFP. Optimizations of nanostructures (MWCNT-COOH, AuNP) and polymers (PAADES) were performed. The MWCNT-COOH, PAADES, and AuNP-modified SPCEs were characterized by FE-SEM-EDX, FTIR, and XRD techniques. The amounts of 6-MOH, APTES, GA, and anti-AFP used to prepare AFP biosensors and the times for anti-AFP and AFP were also optimized. The linear range and detection limit of the AFP biosensors were established as 0.5-250 ng\/mL and 0.15 ng\/mL, respectively. Stability and shelf life were monitored for 60 days and 24 weeks, respectively. The AFP biosensors exhibited high selectivity (97.5%) for AFP in the presence of other cancer biomarkers, including cancer antigen 125 and human epididymis protein 4. Reproducibility tests of the AFP biosensors demonstrated responsiveness across five replicates (RSD: 4.80%, n = 5). Although designed to be disposable, the developed AFP biosensors were reusable up to two times. AFP levels in commercially obtained blood serum were measured by DPV for 2 minutes using AFP biosensors. The developed AFP biosensors are potential candidates for use in point-of-care testing.<\/jats:p>","DOI":"10.55730\/1300-0527.3797","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T05:08:06Z","timestamp":1782450486000},"page":"271-284","source":"Crossref","is-referenced-by-count":0,"title":["Electrochemical biosensing of alpha-fetoprotein based on carboxylated multi-walled nanotube-polyAzur A (in DES)-gold nanoparticles"],"prefix":"10.55730","volume":"50","author":[{"given":"MERVE","family":"YILMAZ \u00c7IL\u00c7AR","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MEL\u0130KE","family":"B\u0130LG\u0130 KAMA\u00c7","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"34691","published-online":{"date-parts":[[2026,6,18]]},"container-title":["Turkish Journal of Chemistry"],"original-title":[],"language":"en","deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:52:52Z","timestamp":1792587172000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.tubitak.gov.tr\/chem\/vol50\/iss3\/5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,18]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,6,18]]}},"URL":"https:\/\/doi.org\/10.55730\/1300-0527.3797","relation":{},"ISSN":["1300-0527"],"issn-type":[{"value":"1300-0527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,18]]}}}