{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T12:24:55Z","timestamp":1706185495535},"reference-count":0,"publisher":"The Nencki Institute of Experimental Biology, Polish Academy of Sciences","license":[{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-sa\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Neurobiol Exp (Wars)"],"abstract":"<jats:p>Pachymic acid (PA) plays a neuroprotective role during cerebral ischemia\/reperfusion. However, the protective mechanisms of PA\nin cerebral ischemia\/reperfusion have been not fully determined. This investigation aims to explore the neuroprotective role of\nPA in ischemia\/reperfusion via miR\u2011155\/NRF2\/HO\u20111 axis. The N2a cell line was induced by hypoxia\/reoxygenation (H\/R) to simulate\nthe neuronal damage that occurs during cerebral ischemia\/reperfusion. PA was used to treat H\/R\u2011induced N2a cells. An MTT assay\nwas used to determine cell viability. The protein levels of Bcl\u20112, Bax, heme oxygenase\u20111 (HO\u20111) and nuclear factor E2\u2011related factor\n2 (NRF2) were measured via Western blot analysis. The level of apoptosis of N2a cells was determined by flow cytometry. The\nexpression levels of miR\u2011155 and NRF2 were quantified by real\u2011ti me PCR. PA treatment inhibits the increase in apoptosis induced\nby H\/R and also enhances the viability of cells exposed to H\/R. PA reverses the increased expression of miR\u2011155 caused by H\/R.\nFurthermore, H\/R does not change the expression of HO\u20111 and NRF2, but PA upregulates the expressions of HO\u20111 and NRF2.\nAdditionally, NRF2 is the target of miR\u2011155. Inhibiting miR\u2011155 contributes to increased cell viability and decreased apoptosis via\ntargeting the NRF2\/HO\u20111 pathway. Overall, PA prevents neuronal cell damage induced by hypoxia\/reoxygenation via miR\u2011155\/\nNRF2\/HO\u20111 axis.<\/jats:p>","DOI":"10.55782\/ane-2022-018","type":"journal-article","created":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T13:36:47Z","timestamp":1657805807000},"source":"Crossref","is-referenced-by-count":4,"title":["Pachymic acid prevents neuronal cell damage induced by hypoxia\/reoxygenation via miR\u2011155\/NRF2\/HO\u20111 axis"],"prefix":"10.55782","author":[{"given":"Yang","family":"Zhai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bugu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoping","family":"Mei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueni","family":"Mo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"34635","published-online":{"date-parts":[[2022,6,30]]},"container-title":["Acta Neurobiologiae Experimentalis"],"original-title":[],"link":[{"URL":"https:\/\/ane.pl\/pdf\/MNczVegKYqTFQyuC.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ane.pl\/pdf\/MNczVegKYqTFQyuC.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T10:45:40Z","timestamp":1691145940000},"score":1,"resource":{"primary":{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/view\/2340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,30]]},"references-count":0,"URL":"https:\/\/doi.org\/10.55782\/ane-2022-018","relation":{},"ISSN":["1689-0035","0065-1400"],"issn-type":[{"value":"1689-0035","type":"electronic"},{"value":"0065-1400","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6,30]]}}}