{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T16:36:38Z","timestamp":1783355798400,"version":"3.54.6"},"reference-count":0,"publisher":"The Nencki Institute of Experimental Biology, Polish Academy of Sciences","issue":"3","license":[{"start":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T00:00:00Z","timestamp":1695945600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Neurobiol. Exp."],"abstract":"<jats:p>The function of microRNA (miRNA) in neuropathic pain (NP) has received widespread attention. The current research sought to address the contribution of miR\u2011488\u20113p in NP and its downstream mechanisms. The NP rat model was constructed by chronic constriction injury (CCI) surgery in rats. Regulation of miR\u2011488\u20113p or Rho\u2011associated coiled\u2011coil\u2011containing protein kinase 1 (ROCK1) in rats by intrathecal injection of lentivirus or plasmid. Real\u2011time quantitative reverse transcription polymerase chain reaction (RT\u2011qPCR) to examine the levels of miR\u2011488\u20113p and ROCK1 in the dorsal root ganglion (DRG). Enzyme\u2011linked immunosorbent assay (ELISA) to monitor the secretion of pro\u2011inflammatory and anti\u2011inflammatory factors. Paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) for the evaluation of mechanosensitive and thermal nociceptive hypersensitivity of NP behaviors. Validation of molecular mechanism\u00a0 between miR\u2011488\u20113p and ROCK1 using RNA immunoprecipitation assay and dual\u2011luciferase reporter (DLR) assay. miR\u2011488\u20113p was vigorously less expressed in the DRGs of CCI rats, while ROCK1 was upregulated. Elevated miR\u2011488\u20113p alleviated the decrease of PWL and PWT in CCI rats, inhibited the secretion of pro\u2011inflammatory factors, and enhanced anti\u2011inflammatory factors levels. Mechanistically, ROCK1 was the target of miR\u2011488\u20113p. Raised ROCK1 partially attenuated the mitigating effect of miR\u2011488\u20113p on NP behavior and the suppression of inflammatory responses in rats. Current research demonstrated that miR\u2011488\u20113p may be a novel therapeutic target for NP.<\/jats:p>","DOI":"10.55782\/ane-2023-2432","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T13:25:32Z","timestamp":1697030732000},"page":"271-279","source":"Crossref","is-referenced-by-count":8,"title":["miR\u2011488\u20113p alleviates neuropathic pain by regulating target gene ROCK1"],"prefix":"10.55782","volume":"83","author":[{"given":"Qiang","family":"Fu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanqing","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"34635","published-online":{"date-parts":[[2023,9,29]]},"container-title":["Acta Neurobiologiae Experimentalis"],"original-title":[],"link":[{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2432\/2401","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2432\/2401","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T11:23:38Z","timestamp":1697801018000},"score":1,"resource":{"primary":{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/view\/2432"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,29]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,9,29]]}},"URL":"https:\/\/doi.org\/10.55782\/ane-2023-2432","relation":{},"ISSN":["1689-0035","0065-1400"],"issn-type":[{"value":"1689-0035","type":"electronic"},{"value":"0065-1400","type":"print"}],"subject":[],"published":{"date-parts":[[2023,9,29]]}}}