{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T04:05:48Z","timestamp":1744430748779,"version":"3.40.4"},"reference-count":0,"publisher":"The Nencki Institute of Experimental Biology, Polish Academy of Sciences","license":[{"start":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T00:00:00Z","timestamp":1741305600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Neurobiol. Exp."],"abstract":"<jats:p>Spontaneous synaptic activity mediated by GABAA receptor is associated with epileptogenicity in focal cortical dysplasia (FCD). miRNAs are potentially involved in the regulation of GABAA receptor subunit expression and activity. This study aimed to determine the expression of miRNAs in FCD and correlate their expression level with mRNA levels of GABAA receptor subunits. Expression of GABAA receptor subunits (\u03b11 and \u03b14) and miRNAs (miR\u2011155\u20115p, miR\u2011186\u20115p, and miR\u201124\u20113p) were evaluated using real\u2011time PCR in resected brain samples from FCD patients. miRNA levels were also determined in the serum of FCD patients. Spontaneous GABAA receptor\u2011mediated synaptic activity was measured using patch clamp technique. Significant increase in \u03b11 and \u03b14 subunit expression and miR\u2011155\u20115p levels, while decrease in miR\u201124\u20113p and miR\u2011186\u20115p levels, was observed in the brain samples of FCD. In the serum of FCD patients, miR\u2011155\u20115p levels were increased, whereas miR\u201124\u20113p and miR\u2011186\u20115p levels remained unaltered. Increased \u03b14 subunit expression in FCD might be due to reduced levels of miR\u201124\u20113p and miR\u2011186\u20115p. In addition, reduced miR\u2011186\u20115p levels might be responsible for increased expression of \u03b11 subunit. We also observed an increase in the spontaneous GABAA receptor\u2011mediated synaptic transmission in FCD. In conclusion, dysregulation of miRNAs and GABAA receptor expression suggest that these miRNAs may contribute to altered GABAA receptor\u2011mediated synaptic activity in FCD.<\/jats:p>","DOI":"10.55782\/ane-2024-2656","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T08:11:38Z","timestamp":1744359098000},"source":"Crossref","is-referenced-by-count":0,"title":["Dysregulation of miR\u201124\u20113p and miR\u2011186\u20115p and GABA&lt;sub&gt;A&lt;\/sub&gt; receptor expression in focal cortical dysplasia"],"prefix":"10.55782","author":[{"given":"Arpna","family":"Srivastava","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yogesh","family":"Agarwal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soumil","family":"Dey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramesh","family":"Doddamani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehar Chand","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manjari","family":"Tripathi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poodipedi Sarat","family":"Chandra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjeev","family":"Lalwani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aparna Banerjee","family":"Dixit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyotirmoy","family":"Banerjee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"34635","published-online":{"date-parts":[[2025,3,7]]},"container-title":["Acta Neurobiologiae Experimentalis"],"original-title":[],"link":[{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2656\/2505","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2656\/2505","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T08:11:38Z","timestamp":1744359098000},"score":1,"resource":{"primary":{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/view\/2656"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,7]]},"references-count":0,"URL":"https:\/\/doi.org\/10.55782\/ane-2024-2656","relation":{},"ISSN":["1689-0035","0065-1400"],"issn-type":[{"value":"1689-0035","type":"electronic"},{"value":"0065-1400","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3,7]]}}}