{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T11:03:06Z","timestamp":1767006186064,"version":"3.48.0"},"reference-count":0,"publisher":"The Nencki Institute of Experimental Biology, Polish Academy of Sciences","issue":"3-4","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Neurobiol. Exp."],"abstract":"<jats:p>Angiotensin\u2011(1\u20117) [Ang\u2011(1\u20117)] exerts physiological effects in the brain mediated by its receptor, Mas. Recent studies have successfully demonstrated that Ang\u2011(1\u20117) exerts neuroprotective effects following cerebral ischemia in a rat model. However, prior investigations utilized direct intracerebral cannulation for Ang\u2011(1\u20117) delivery, potentially limiting human application. Hematopoietic stem cells (HSC) have been previously demonstrated to mobilize to the site of cerebral injury in response to stroke. Therefore, we sought to examine the therapeutic potential of HSC transduced via a lentivirus with Ang\u2011(1\u20117) to migrate to the ischemic hemisphere and overexpress Ang\u2011(1\u20117) following stroke. Animals were divided into 3 groups: Stroke + PBS, Stroke + HSC, Stroke + Ang\u2011(1\u20117)\u2011transduced HSC. Bone marrow from separate animals was harvested and used for injection of the HSC, with or without lentivirus induced Ang\u2011(1\u20117) transduction. A neurological assessment was performed at 72 hours post\u2011surgery. Ang\u2011(1\u20117) transduced HSC secreted the peptide up to 72 hours post infection, in vitro. Stroked animals injected with the Ang\u2011(1\u20117) infected HSC exhibited reduced behavioral deficits on the Bederson neurological assessment scale. These data suggest that HSC\u2011mediated delivery of Ang\u2011(1\u20117) to ischemic brain appears to improve post\u2011stroke outcomes and may offer a novel route of therapeutic agent delivery to the brain.<\/jats:p>","DOI":"10.55782\/gqtr4n50","type":"journal-article","created":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T13:13:29Z","timestamp":1766841209000},"page":"128-137","source":"Crossref","is-referenced-by-count":0,"title":["Hematopoietic stem cell\u2011based angiotensin\u2011(1\u20117) delivery to the brain improves functional outcomefollowing cerebral ischemia"],"prefix":"10.55782","volume":"85","author":[{"given":"Aqeela","family":"Afzal","sequence":"first","affiliation":[{"name":"Department of Neurological Surgery, Division of Surgical Sciences, Vanderbilt University Medical Center, Nashville, TN, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fiona","family":"Desland","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Mount Sinai Hospital, New York, NY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongwei","family":"Li","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Regenhardt","sequence":"additional","affiliation":[{"name":"Departments of Neurosurgery and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Caldwell","sequence":"additional","affiliation":[{"name":"Cancer and Blood Disorders Institute, Johns Hopkins All Children\u2019s Hospital, St. Petersburg, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Barbay","sequence":"additional","affiliation":[{"name":"Department of Physical Medicine and Rehabilitation, University of Kansas Medical Center, Kansas City, Kansas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Mocco","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Mount Sinai Hospital, New York, NY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"34635","published-online":{"date-parts":[[2025,12,3]]},"container-title":["Acta Neurobiologiae Experimentalis"],"original-title":[],"link":[{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2630\/2537","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2630\/2537","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T10:58:04Z","timestamp":1767005884000},"score":1,"resource":{"primary":{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/view\/2630"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,3]]},"references-count":0,"journal-issue":{"issue":"3-4","published-online":{"date-parts":[[2025,12,29]]}},"URL":"https:\/\/doi.org\/10.55782\/gqtr4n50","relation":{},"ISSN":["1689-0035","0065-1400"],"issn-type":[{"type":"electronic","value":"1689-0035"},{"type":"print","value":"0065-1400"}],"subject":[],"published":{"date-parts":[[2025,12,3]]}}}