{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T10:08:51Z","timestamp":1772014131534,"version":"3.50.1"},"reference-count":0,"publisher":"The Nencki Institute of Experimental Biology, Polish Academy of Sciences","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Neurobiol. Exp."],"abstract":"<jats:p>Large conductance cation channels (LCC\u2011channels), located in the nuclear membrane, are potential mediators of potassium (K+) countercurrent during calcium ion (Ca2+) release from intracellular stores. This study examined the effects of epinephrine, norepinephrine, isoprenaline, and propranolol on the electrophysiological properties of these channels to evaluate their potential as blocking agents in future studies of LCC\u2011channel involvement in Ca2+ release. The patch\u2011clamp method, using voltage\u2011clamp mode and a nucleus\u2011attached configuration, was employed to record currents passing through LCC\u2011channels in Purkinje cell nuclei. The effectiveness of Ca2+ blocking was estimated by analyzing changes in current amplitude and the probability of the channels being in an open state. All tested adrenergic receptor modulators decreased current amplitude through LCC\u2011channels at negative membrane potentials to varying extents, while no changes in amplitude were found for positive applied potentials for any compound. Epinephrine, norepinephrine, and propranolol demonstrated blocking capabilities comparable to nicotinic acetylcholine receptor (nAChR) modulators under similar conditions. Notably, only norepinephrine significantly inhibited the open\u2011state probability of LCC\u2011channels, whereas isoprenaline increased this parameter and induced rapid flickering. Furthermore, isoprenaline produced a greater reduction in current amplitude through LCC\u2011channels than the other compounds.<\/jats:p>","DOI":"10.55782\/j42ygh03","type":"journal-article","created":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T09:15:33Z","timestamp":1772010933000},"source":"Crossref","is-referenced-by-count":0,"title":["Effects of adrenergic receptor modulators on nuclear LCC\u2011channels in Purkinje neurons"],"prefix":"10.55782","author":[{"given":"Olena","family":"Kotyk","sequence":"first","affiliation":[{"name":"Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serhii","family":"Nadtoka","sequence":"additional","affiliation":[{"name":"Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetiana","family":"Vynohradova","sequence":"additional","affiliation":[{"name":"Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amelia","family":"Taghavi","sequence":"additional","affiliation":[{"name":"Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serhii","family":"Marchenko","sequence":"additional","affiliation":[{"name":"Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Kotliarova","sequence":"additional","affiliation":[{"name":"Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"34635","published-online":{"date-parts":[[2026,2,23]]},"container-title":["Acta Neurobiologiae Experimentalis"],"original-title":[],"link":[{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2762\/2544","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2762\/2544","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T09:15:33Z","timestamp":1772010933000},"score":1,"resource":{"primary":{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/view\/2762"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,23]]},"references-count":0,"URL":"https:\/\/doi.org\/10.55782\/j42ygh03","relation":{},"ISSN":["1689-0035","0065-1400"],"issn-type":[{"value":"1689-0035","type":"electronic"},{"value":"0065-1400","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2,23]]}}}