{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:04:36Z","timestamp":1789383876576,"version":"build-2803163510"},"reference-count":0,"publisher":"The Nencki Institute of Experimental Biology, Polish Academy of Sciences","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Neurobiol. Exp."],"abstract":"<jats:p>It is well established that the circadian clock has pronounced effects on functioning of cells, tissues and body organs. Little, however, is known regarding its role in the body\u2019s adaptation to physical activity. The aim of the present study was to establish the effect of physical activity\u2014performed at either the activity peak (ZT1) in the morning or during \u2018siesta\u2019 time (ZT6) in the middle of the day\u2014on the lifespan of male and female Drosophila melanogaster. Three\u2011day\u2011old virgin flies (separated by sex) were allocated to control or exercise groups. Flies in the exercise groups were subjected to exercise every day by applying motor stimulation at ZT1 or at ZT6. Overall lifetime physical activity of controls was lower in male D. melanogaster compared to females. Forced physical activity at ZT1 prolonged the male lifespan by ~35%, whereas females in the same group exhibited a lifespan reduction of ~84%. In contrast, physical activity performed at \u2018siesta\u2019 time shortened the lifespan of both male and female flies, by ~20% and ~67%, respectively. Forced locomotor activity performed during the time of\u00a0peak natural daily activity prolonged life expectancy in males but had a negative effect on female longevity. Interestingly, when physical activity was applied during the \u2018siesta\u2019 time, it shortened the lifespan regardless of sex. Therefore, our study suggests that synchronizing physical activity to the daily rhythm of activity and rest dictated by the circadian clock could be a new strategy for optimizing the effects of physical training. However, this interpretation requires further research involving human subjects of different chronotypes.<\/jats:p>","DOI":"10.55782\/my5gz879","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:20:45Z","timestamp":1789381245000},"source":"Crossref","is-referenced-by-count":0,"title":["The circadian clock: an important factor modifying the effect of physical activity on the lifespan of\u00a0&lt;i&gt;Drosophila melanogaster &lt;\/i&gt;&lt;b&gt;&lt;i&gt;&lt;\/i&gt;&lt;\/b&gt;"],"prefix":"10.55782","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7096-3134","authenticated-orcid":true,"given":"Lucyna","family":"Widacha","sequence":"first","affiliation":[{"name":"Chair of Exercise Physiology and Muscle Bioenergetics, Faculty of Health Sciences, Jagiellonian University Medical College, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Majerczyk","sequence":"additional","affiliation":[{"name":"Chair of Exercise Physiology and Muscle Bioenergetics, Faculty of Health Sciences, Jagiellonian University Medical College, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Szramel","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Jagiellonian University, Krakow, Poland; Laboratory of Neuropharmacology and Epigenetics, Department of Drug Addiction Pharmacology, Maj Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wojciech","family":"Krzeptowski","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropharmacology and Epigenetics, Department of Drug Addiction Pharmacology, Maj Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerzy A.","family":"Zoladz","sequence":"additional","affiliation":[{"name":"Chair of Exercise Physiology and Muscle Bioenergetics, Faculty of Health Sciences, Jagiellonian University Medical College, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1503-9533","authenticated-orcid":true,"given":"Elzbieta","family":"Pyza","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Jagiellonian University, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"34635","published-online":{"date-parts":[[2026,9,14]]},"container-title":["Acta Neurobiologiae Experimentalis"],"original-title":[],"deposited":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:20:46Z","timestamp":1789381246000},"score":1,"resource":{"primary":{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/view\/2791"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,14]]},"references-count":0,"URL":"https:\/\/doi.org\/10.55782\/my5gz879","relation":{},"ISSN":["1689-0035","0065-1400"],"issn-type":[{"value":"1689-0035","type":"electronic"},{"value":"0065-1400","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9,14]]}}}