{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T10:59:32Z","timestamp":1767005972826,"version":"3.48.0"},"reference-count":0,"publisher":"The Nencki Institute of Experimental Biology, Polish Academy of Sciences","issue":"3-4","license":[{"start":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:00:00Z","timestamp":1760918400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Neurobiol. Exp."],"abstract":"<jats:p>The present study aims to elucidate the role of the Sigma\u20111 receptor in the pathogenesis of neuropathic pain and evaluate its potential therapeutic implications. To systematically assess the effects of the Sigma\u20111 receptor, neuropathic pain was induced in rats using the chronic constriction injury (CCI) model. Subjects were subsequently divided into three groups: Sham, CCI, and CCI+BD1047 (where BD1047 is a Sigma\u20111 receptor antagonist). Following intrathecal administration of the respective agents, thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) were measured. Additionally, Western blotting was utilized to examine Sigma\u20111 receptor, phosphorylated protein kinase C\u03b1 (p\u2011PKC\u03b1), and P2X3 receptor expression in the dorsal root ganglia (DRG). Immunofluorescence techniques were employed to examine p\u2011PKC\u03b1 and P2X3 receptor expression. The results indicate a direct correlation between Sigma\u20111 receptor activity and pain perception, evidenced by changes in TWL and MWT. In the CCI group, both TWL and MWT were significantly reduced compared to the Sham group. Furthermore, protein levels of the Sigma\u20111 receptor, p\u2011PKC\u03b1, and P2X3 receptor in the DRG were elevated, and immunofluorescence expression of p\u2011PKC\u03b1 and the P2X3 receptor also increased. Conversely, in the CCI+BD1047 group, TWL and MWT were significantly enhanced. Additionally, protein levels of the Sigma\u20111 receptor, p\u2011PKC\u03b1, and P2X3 receptor in the DRG decreased, along with reduced immunofluorescence expression of p\u2011PKC\u03b1 and P2X3 receptor. The findings indicate that neuropathic pain is intricately associated with the Sigma\u20111 receptor, p\u2011PKC\u03b1, and P2X3 receptor in the dorsal root ganglia. Notably, the Sigma\u20111 receptor regulates the expression of p\u2011PKC\u03b1 and P2X3 receptor, presenting a novel therapeutic target for neuropathic pain management.<\/jats:p>","DOI":"10.55782\/tymtpb63","type":"journal-article","created":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T13:14:55Z","timestamp":1766841295000},"page":"119-127","source":"Crossref","is-referenced-by-count":0,"title":["Sigma\u20111 receptor regulates p\u2011PKC\u03b1 and P2X3 expression in dorsal root ganglia to attenuate neuropathic pain in rats"],"prefix":"10.55782","volume":"85","author":[{"given":"Shuang-shuang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pain Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China"},{"name":"Guizhou Key Laboratory of Basic Research on Anesthesia and Organ Protection, Zunyi, Guizhou, China"},{"name":"Department of Anesthesiology, Daping Hospital, Army Medical University, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Hospital of Stomatology, Zunyi Medical University, Zunyi, Guizhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-mei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-xiang","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Daping Hospital, Army Medical University, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Pain Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China"},{"name":"Guizhou Key Laboratory of Basic Research on Anesthesia and Organ Protection, Zunyi, Guizhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bang-yong","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Pain Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"34635","published-online":{"date-parts":[[2025,10,20]]},"container-title":["Acta Neurobiologiae Experimentalis"],"original-title":[],"link":[{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2699\/2535","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2699\/2535","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T10:57:41Z","timestamp":1767005861000},"score":1,"resource":{"primary":{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/view\/2699"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,20]]},"references-count":0,"journal-issue":{"issue":"3-4","published-online":{"date-parts":[[2025,12,29]]}},"URL":"https:\/\/doi.org\/10.55782\/tymtpb63","relation":{},"ISSN":["1689-0035","0065-1400"],"issn-type":[{"type":"electronic","value":"1689-0035"},{"type":"print","value":"0065-1400"}],"subject":[],"published":{"date-parts":[[2025,10,20]]}}}