{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T08:58:26Z","timestamp":1781513906623,"version":"3.54.1"},"reference-count":0,"publisher":"The Nencki Institute of Experimental Biology, Polish Academy of Sciences","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Neurobiol. Exp."],"abstract":"<jats:p>In vivo electrophysiological experiments were conducted to evaluate the effects of intraventricular and cortical administration of orexins on electrically evoked epileptiform discharges (EEDs) in the hippocampal CA1 field. Bipolar electrodes were stereotaxically implanted into the CA1 region of anesthetized rats, and epileptiform activity was induced by high\u2011frequency electrical stimulation (HFES). Orexin\u2011A and orexin\u2011B (10 \u03bcg\/10 \u03bcl) were administered into the lateral ventricle or applied to the frontal cortex. Intracerebroventricular administration of orexin\u2011A significantly reduced the duration of EEDs within 20 minutes and abolished the progressive, HFES\u2011related prolongation of epileptiform discharges observed in control animals. In contrast, cortical administration of orexin\u2011A selectively reduced the incidence of high\u2011amplitude (7\u201310 mV) population spikes during EEDs without affecting the duration of epileptiform activity. Neither cortical nor intraventricular administration of orexin\u2011B produced significant changes in evoked epileptiform activity in the CA1 field, suggesting a possible involvement of OX1 receptor signaling in mediating the effects of orexin\u2011A. The differential effects observed after cortical versus intraventricular administration of orexin\u2011A appear to reflect site\u2011specific mechanisms of action. The effects of intraventricular administration of orexin\u2011A were predominantly induced via direct activation of hippocampal orexinergic receptors, whereas cortical application of orexin\u2011A induced its effects via modulation of cortico\u2011hippocampal mechanisms.<\/jats:p>","DOI":"10.55782\/tyq4vx54","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T08:44:43Z","timestamp":1781513083000},"source":"Crossref","is-referenced-by-count":0,"title":["Modulation of electrically evoked hippocampal epileptiform activity by exogenous orexins in the rat CA1 field"],"prefix":"10.55782","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6909-412X","authenticated-orcid":true,"given":"Tsira","family":"Kapanadze","sequence":"first","affiliation":[{"name":"Department of Biology, Iv. Javakhishvili Tbilisi State University, Faculty of Exact and Natural Science, Tbilisi, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariam","family":"Kurasbediani","sequence":"additional","affiliation":[{"name":"Department of Biology, Iv. Javakhishvili Tbilisi State University, Faculty of Exact and Natural Science, Tbilisi, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1719-3717","authenticated-orcid":true,"given":"Gia","family":"Kutelia","sequence":"additional","affiliation":[{"name":"Department of Biology, Iv. Javakhishvili Tbilisi State University, Faculty of Exact and Natural Science, Tbilisi, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1718-313X","authenticated-orcid":true,"given":"Butsiko","family":"Chkhartishvili","sequence":"additional","affiliation":[{"name":"Department of Biology, Iv. Javakhishvili Tbilisi State University, Faculty of Exact and Natural Science, Tbilisi, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3973-5322","authenticated-orcid":true,"given":"Nanuli","family":"Doreulee","sequence":"additional","affiliation":[{"name":"Department of Biology, Iv. Javakhishvili Tbilisi State University, Faculty of Exact and Natural Science, Tbilisi, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"34635","published-online":{"date-parts":[[2026,6,15]]},"container-title":["Acta Neurobiologiae Experimentalis"],"original-title":[],"link":[{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2784\/2565","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/download\/2784\/2565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T08:44:45Z","timestamp":1781513085000},"score":1,"resource":{"primary":{"URL":"https:\/\/ane.pl\/index.php\/ane\/article\/view\/2784"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,15]]},"references-count":0,"URL":"https:\/\/doi.org\/10.55782\/tyq4vx54","relation":{},"ISSN":["1689-0035","0065-1400"],"issn-type":[{"value":"1689-0035","type":"electronic"},{"value":"0065-1400","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6,15]]}}}