{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:52:55Z","timestamp":1762005175886},"reference-count":0,"publisher":"International Union Against Tuberculosis and Lung Disease","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["int j tuberc lung dis"],"published-print":{"date-parts":[[2019,12,1]]},"abstract":"<jats:p><jats:bold>SETTING:<\/jats:bold> Patients with presumed multidrug-resistant tuberculosis (MDR-TB) and undergoing MDR-TB treatment from Rajasthan, India.<jats:bold>OBJECTIVE:<\/jats:bold> To compare the GenoType<jats:sup>\u00ae<\/jats:sup> MTBDR<jats:italic>sl<\/jats:italic> v.1.0 (MTBDR<jats:italic>sl<\/jats:italic>) assay capacity to detect resistance to ofloxacin,\n amikacin, capreomycin, kanamycin and ethambutol in <jats:italic>Mycobacterium tuberculosis<\/jats:italic> with phenotypic drug susceptibility testing (DST) using MGIT<jats:sup>\u2122<\/jats:sup>960\u2122 in sputum samples and isolates.<jats:bold>DESIGN:<\/jats:bold> Fifty-three smear-positive sputum samples were tested directly by\n MTBDR<jats:italic>sl<\/jats:italic> and 205 MDR-TB isolates were processed using MTBDR<jats:italic>sl<\/jats:italic> and DST for five drugs on MGIT960. DNA sequencing was performed in isolates with discordance in the results between the two methods for the <jats:italic>gyr<\/jats:italic>A<jats:italic>, gyr<\/jats:italic>B and <jats:italic>rrs genes<\/jats:italic>.<jats:bold>RESULT:<\/jats:bold> Sensitivity\n and specificity of MTBDR<jats:italic>sl<\/jats:italic> was found to be respectively 93.1% and 100% for fluoroquinoline, respectively 75\u201378% and 100% for aminoglycosides\/cyclopeptides, respectively 70% and 92% for ethambutol and respectively 92.3% and 100% for extensively drug-resistant (XDR) TB detection.\n On sequencing eight discordant isolates for quinolones, mutations were seen in 12.5% of the <jats:italic>gyr<\/jats:italic>B gene and among 20 discordant isolates for aminoglycosides\/cyclopeptides in the <jats:italic>rrs<\/jats:italic> gene in 15% isolates. The turnaround time was 2 days for MTBDR<jats:italic>sl<\/jats:italic> vs. 10 days for MGIT960.<jats:bold>CONCLUSIONS:<\/jats:bold>\n MTBDR<jats:italic>sl<\/jats:italic> can be used as an initial rapid test for detecting XDR-TB, resistance to quinolones and aminogycosides\/cyclopeptides in smear-positive sputum samples.<\/jats:p>","DOI":"10.5588\/ijtld.18.0562","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T10:28:49Z","timestamp":1578911329000},"page":"1257-1262","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of MTBDR<i>sl<\/i> for detecting resistance in <i>Mycobacterium tuberculosis<\/i> to second-line drugs"],"prefix":"10.5588","volume":"23","author":[{"given":"R. J.","family":"Chandak","sequence":"first","affiliation":[{"name":"Gobind Ballabh Pant Institute of Post Graduate Medical Education and Research, New Delhi"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Malhotra","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sawai Man Singh Medical College, Jaipur, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Bhargava","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sawai Man Singh Medical College, Jaipur, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. K.","family":"Goel","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sawai Man Singh Medical College, Jaipur, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Verma","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sawai Man Singh Medical College, Jaipur, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sawai Man Singh Medical College, Jaipur, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3554","container-title":["The International Journal of Tuberculosis and Lung Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/iuatld\/ijtld\/2019\/00000023\/00000012\/art00006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,11]],"date-time":"2021-03-11T04:33:29Z","timestamp":1615437209000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.5588\/ijtld.18.0562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,1]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12,1]]}},"alternative-id":["1027-3719(20191201)23:12L.1257;1-"],"URL":"https:\/\/doi.org\/10.5588\/ijtld.18.0562","relation":{},"ISSN":["1027-3719"],"issn-type":[{"value":"1027-3719","type":"print"}],"subject":[],"published":{"date-parts":[[2019,12,1]]}}}