{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T01:19:37Z","timestamp":1773364777613,"version":"3.50.1"},"reference-count":0,"publisher":"International Union Against Tuberculosis and Lung Disease","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["int j tuberc lung dis"],"published-print":{"date-parts":[[2020,2,1]]},"abstract":"<jats:p><jats:bold>SETTING:<\/jats:bold> QuantiFERON TB assay (QFT) is used to screen tuberculosis (TB) infection, but it cannot distinguish active TB from latent TB infection (LTBI).<jats:bold>OBJECTIVE:<\/jats:bold> To evaluate the quantitative expression of the high-affinity FCgamma receptor I (CD64) on neutrophils (NE)\n and monocytes (MO) in peripheral blood using flow cytometry, measured in antibody binding capacity (ABC) units as a predictive biomarker of TB.<jats:bold>DESIGN:<\/jats:bold> Fifty-two patients were enrolled (45 QFT-positive and 7 QFT-indeterminate). Cultures and molecular analyses were performed.<jats:bold>RESULTS:<\/jats:bold>\n Of the 45 QFT-positive patients, 29 were culture-positive (active TB) and 16 were negative (LTBI). The median NE CD64 ABC and MO CD64 ABC expression was significantly higher (<jats:italic>P<\/jats:italic> &lt; 0.001) in culture-positive patients. The NE CD64 and MO CD64 area under the receiver operating characteristic\n curve values were respectively 0.948 (95%CI 0.838\u20130.992) and 0.989 (0.901\u20131.000). By setting the cut-off NE CD64 value at &gt;2400 ABC or MO CD64 value &gt;25\u2009800 the assay sensitivity increased to 95.5% with 100% specificity and 100% positive predictive value. In the QFT-indeterminate\n group, five culture-positive cases had NE CD64 &gt;2400 ABC or MO CD64 value &gt;25\u2009800; two culture-negative cases had lower values.<jats:bold>CONCLUSION:<\/jats:bold> The CD64 quantitative expression on peripheral blood cells may be used as a predictive biomarker for active TB.<\/jats:p>","DOI":"10.5588\/ijtld.19.0147","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T04:39:41Z","timestamp":1583296781000},"page":"196-201","source":"Crossref","is-referenced-by-count":12,"title":["Quantification of neutrophil and monocyte CD64 expression: a predictive biomarker for active tuberculosis"],"prefix":"10.5588","volume":"24","author":[{"given":"A.","family":"Gatti","sequence":"first","affiliation":[{"name":"Haematology Laboratory and Transfusion Centre"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Ceriani","sequence":"additional","affiliation":[{"name":"Microbiology Unit"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"De Paschale","sequence":"additional","affiliation":[{"name":"Microbiology Unit"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Magnani","sequence":"additional","affiliation":[{"name":"Infectious Diseases Unit, Legnano General Hospital, Legnano, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Villa","sequence":"additional","affiliation":[{"name":"Infectious Diseases Unit, Legnano General Hospital, Legnano, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Vigan\u00f2","sequence":"additional","affiliation":[{"name":"Infectious Diseases Unit, Legnano General Hospital, Legnano, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Clerici","sequence":"additional","affiliation":[{"name":"Microbiology Unit"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Brando","sequence":"additional","affiliation":[{"name":"Haematology Laboratory and Transfusion Centre"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3554","container-title":["The International Journal of Tuberculosis and Lung Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/iuatld\/ijtld\/2020\/00000024\/00000002\/art00008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T04:39:47Z","timestamp":1583296787000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.5588\/ijtld.19.0147"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2,1]]}},"alternative-id":["1027-3719(20200201)24:2L.196;1-"],"URL":"https:\/\/doi.org\/10.5588\/ijtld.19.0147","relation":{},"ISSN":["1027-3719"],"issn-type":[{"value":"1027-3719","type":"print"}],"subject":[],"published":{"date-parts":[[2020,2,1]]}}}