{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T12:22:21Z","timestamp":1764937341232},"reference-count":0,"publisher":"International Union Against Tuberculosis and Lung Disease","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["int j tuberc lung dis"],"published-print":{"date-parts":[[2021,9,1]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Whole-genome sequencing (WGS) is a promising tool for the detection of drug-resistant TB (DR-TB). To date, there have been few comparisons of diagnostic performance of WGS and phenotypic drug susceptibility testing (DST) in DR-TB.<jats:bold>METHODS:<\/jats:bold> We compared drug\n resistance-conferring mutations identified by WGS analysis using TB-Profiler and Mykrobe with phenotypic DST profiles based on the L\u00f6wenstein-Jensen proportion method using drug-resistant <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (<jats:italic>n<\/jats:italic> = 537) isolates from across Thailand. Based on available\n phenotypic DST results, diagnostic performance was analysed for resistance against isoniazid, rifampicin, ethambutol (EMB), streptomycin, ethionamide (ETH), kanamycin, capreomycin (CPM), para-aminosalicylic acid, ofloxacin and levofloxacin.<jats:bold>RESULTS:<\/jats:bold> High agreement between the two\n methods was observed for most drugs (&gt;91%), except EMB (57%, 95% CI 53\u201361) and ETH (70%, 95% CI 66\u201374). Also, low specificity was observed for EMB (49%, 95% CI 44\u201354) and ETH (66%, 95% CI 61\u201371). Sensitivity was high for most drugs (range 83\u201398%), except CPM\n (77%, 95% CI 59\u201388).<jats:bold>CONCLUSION:<\/jats:bold> Low agreement between WGS and phenotypic tests for drug resistance was found for EMB and ETH. The current genomic database is insufficient for the identification of CPM resistance. Challenges remain for routine usage of WGS-based DST, especially\n for second-line anti-TB drugs.<\/jats:p>","DOI":"10.5588\/ijtld.21.0096","type":"journal-article","created":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T09:54:00Z","timestamp":1637574840000},"page":"754-760","source":"Crossref","is-referenced-by-count":6,"title":["Diagnostic performance of whole-genome sequencing for identifying drug-resistant TB in Thailand"],"prefix":"10.5588","volume":"25","author":[{"given":"P.","family":"Kamolwat","sequence":"first","affiliation":[{"name":"Division of Tuberculosis, Department of Disease Control, Ministry of Public Health, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Nonghanphithak","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Chaiprasert","sequence":"additional","affiliation":[{"name":"Drug Resistant Tuberculosis Research Fund Laboratory, Research and Development Affairs, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Smithtikarn","sequence":"additional","affiliation":[{"name":"Division of Tuberculosis, Department of Disease Control, Ministry of Public Health, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Pungrassami","sequence":"additional","affiliation":[{"name":"Division of Tuberculosis, Department of Disease Control, Ministry of Public Health, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Faksri","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3554","container-title":["The International Journal of Tuberculosis and Lung Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/iuatld\/ijtld\/2021\/00000025\/00000009\/art00014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T09:54:17Z","timestamp":1637574857000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.5588\/ijtld.21.0096"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,1]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,9,1]]}},"alternative-id":["1027-3719(20210901)25:9L.754;1-"],"URL":"https:\/\/doi.org\/10.5588\/ijtld.21.0096","relation":{},"ISSN":["1027-3719"],"issn-type":[{"value":"1027-3719","type":"print"}],"subject":[],"published":{"date-parts":[[2021,9,1]]}}}