{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T13:54:57Z","timestamp":1723038897181},"reference-count":0,"publisher":"International Union Against Tuberculosis and Lung Disease","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["int j tuberc lung dis"],"published-print":{"date-parts":[[2022,5,1]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> The GenoType MTBDR<jats:italic>sl<\/jats:italic> v2 is a molecular test designed for the rapid detection of resistance to second-line anti-TB drugs in <jats:italic>Mycobacterium tuberculosis<\/jats:italic> complex (MTBC).<jats:bold>OBJECTIVE:<\/jats:bold> To assess the use of MTBDR<jats:italic>sl<\/jats:italic> in a programmatic setting and\n to describe the resistance patterns in a high HIV-TB-endemic area in South Africa.<jats:bold>METHODS:<\/jats:bold> We performed a retrospective data analysis of all MTBDR<jats:italic>sl<\/jats:italic> results in patients with newly diagnosed rifampicin-resistant TB (RR-TB). We compared its performance on direct testing of\n smear-positive and smear-negative specimens. Results were examined to observe the detected resistance-conferring mutations.<jats:bold>RESULTS:<\/jats:bold> Of 1873 RR-TB\/multidrug-resistant TB (MDR-TB), 37.4% were smear-negative and 62.5% were smear-positive. Among smear-negative specimens, the MTBDR<jats:italic>sl<\/jats:italic>\n showed an inconclusive rate of 61.2%, while the inconclusive rate from smear-positive specimens was 6.6%. The most common mutation observed in case of fluoroquinolone resistance occurred at the <jats:italic>gyr<\/jats:italic>A gene, codon 90 (A90V) (61\/158, 38.6%), and the most common mutation in injectable aminoglycoside\n resistance occurred in the <jats:italic>rrs<\/jats:italic> region, A1401G (71\/108, 65.7%).<jats:bold>CONCLUSION:<\/jats:bold> In HIV-TB-prevalent settings, routine use of the MTBDR<jats:italic>sl<\/jats:italic> is more effective when performed directly on smear-positive specimens. In view of currently used injectable-free regimens, this test requires\n revision.<\/jats:p>","DOI":"10.5588\/ijtld.21.0590","type":"journal-article","created":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T04:32:11Z","timestamp":1651638731000},"page":"426-432","source":"Crossref","is-referenced-by-count":1,"title":["Performance of the GenoType MTBDR<i>sl<\/i> in a programmatic setting, South Africa"],"prefix":"10.5588","volume":"26","author":[{"given":"K.","family":"Lutchminarain","sequence":"first","affiliation":[{"name":"National Health Laboratory Service, Durban, South Africa, University of KwaZulu Natal, Durban, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kajee","sequence":"additional","affiliation":[{"name":"National Health Laboratory Service, Durban, South Africa, University of KwaZulu Natal, Durban, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. R.","family":"Gandhi","sequence":"additional","affiliation":[{"name":"Emory University Rollins School of Public Health, Atlanta, GA, Emory University School of Medicine, Atlanta, GA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. S. S.","family":"Han","sequence":"additional","affiliation":[{"name":"National Health Laboratory Service, Durban, South Africa, University of KwaZulu Natal, Durban, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Mvelase","sequence":"additional","affiliation":[{"name":"National Health Laboratory Service, Durban, South Africa, University of KwaZulu Natal, Durban, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3554","container-title":["The International Journal of Tuberculosis and Lung Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/iuatld\/ijtld\/2022\/00000026\/00000005\/art00009","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T04:32:18Z","timestamp":1657686738000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.5588\/ijtld.21.0590"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,5,1]]}},"alternative-id":["1027-3719(20220501)26:5L.426;1-"],"URL":"https:\/\/doi.org\/10.5588\/ijtld.21.0590","relation":{},"ISSN":["1027-3719"],"issn-type":[{"value":"1027-3719","type":"print"}],"subject":[],"published":{"date-parts":[[2022,5,1]]}}}