{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:58:36Z","timestamp":1760986716799},"reference-count":0,"publisher":"International Union Against Tuberculosis and Lung Disease","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["int j tuberc lung dis"],"published-print":{"date-parts":[[2022,11,1]]},"abstract":"<jats:p><jats:bold>SETTING:<\/jats:bold> Mulago Hospital, Kampala, Uganda.<jats:bold>OBJECTIVE:<\/jats:bold> To quantify <jats:italic>Mycobacterium tuberculosis<\/jats:italic> in sputum during the first 8 weeks of pulmonary multidrug-resistant TB (MDR-TB) treatment.<jats:bold>DESIGN:<\/jats:bold> We enrolled consecutive adults with pulmonary MDR-TB treated\n according to national guidelines. We collected overnight sputum samples before treatment and weekly. Sputum samples were cultured on Middlebrook 7H11S agar to measure colony-forming units per mL (cfu\/mL) and in MGIT\u2122 960\u2122 media to measure time to detection (TTD). Linear mixed-effects\n regression was used to estimate the relational change in log<jats:sub>10<\/jats:sub> cfu\/mL and TTD.<jats:bold>RESULTS:<\/jats:bold> Twelve adults (median age: 27 years) were enrolled. Half were women, and two-thirds were HIV-positive. At baseline, median log<jats:sub>10<\/jats:sub> cfu\/mL was 5.1, decreasing by 0.29 log<jats:sub>10<\/jats:sub>\n cfu\/mL\/week. The median TTD was 116.5 h, increasing in TTD by 36.97 h\/week. The weekly change was greater in the first 2 weeks (\u20131.04 log<jats:sub>10<\/jats:sub> cfu\/mL\/week and 120.02 h\/week) than in the remaining 6 weeks (\u20130.17 log<jats:sub>10<\/jats:sub> cfu\/mL\/week and 26.11 h\/week).<jats:bold>CONCLUSION:<\/jats:bold>\n Serial quantitative culture measures indicate a slow, uneven rate of decline in sputum <jats:italic>M. tuberculosis<\/jats:italic> over 8 weeks of standardized pulmonary MDR-TB treatment.<\/jats:p>","DOI":"10.5588\/ijtld.21.0741","type":"journal-article","created":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T04:34:14Z","timestamp":1666672454000},"page":"1058-1064","source":"Crossref","is-referenced-by-count":2,"title":["Quantification of multidrug-resistant <i>M. tuberculosis<\/i> bacilli in sputum during the first 8 weeks of treatment"],"prefix":"10.5588","volume":"26","author":[{"given":"S. E.","family":"Smith-Jeffcoat","sequence":"first","affiliation":[{"name":"Division of Global HIV\/AIDS and Tuberculosis, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. D.","family":"Eisenach","sequence":"additional","affiliation":[{"name":"TB or NOT TB Consulting, Little Rock, AR, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Joloba","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Biomedical Research Center, Makerere University, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Ssengooba","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Biomedical Research Center, Makerere University, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Namaganda","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Biomedical Research Center, Makerere University, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Nsereko","sequence":"additional","affiliation":[{"name":"Uganda-Case Western Research Unit Research Collaboration, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Okware","sequence":"additional","affiliation":[{"name":"Uganda-Case Western Research Unit Research Collaboration, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. S.","family":"Cavanaugh","sequence":"additional","affiliation":[{"name":"Division of Global HIV\/AIDS and Tuberculosis, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. P.","family":"Cegielski","sequence":"additional","affiliation":[{"name":"Division of Global HIV\/AIDS and Tuberculosis, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3554","container-title":["The International Journal of Tuberculosis and Lung Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/iuatld\/ijtld\/2022\/00000026\/00000011\/art00010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T04:35:23Z","timestamp":1666758923000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.5588\/ijtld.21.0741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,1]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11,1]]}},"alternative-id":["1027-3719(20221101)26:11L.1058;1-"],"URL":"https:\/\/doi.org\/10.5588\/ijtld.21.0741","relation":{},"ISSN":["1027-3719"],"issn-type":[{"value":"1027-3719","type":"print"}],"subject":[],"published":{"date-parts":[[2022,11,1]]}}}