{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T00:22:11Z","timestamp":1721953331765},"reference-count":0,"publisher":"International Union Against Tuberculosis and Lung Disease","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["int j tuberc lung dis"],"published-print":{"date-parts":[[2024,8,1]]},"abstract":"<jats:p>&lt;sec&gt;&lt;title&gt;BACKGROUND&lt;\/title&gt;This study evaluated the diagnostic performance of the thin-layer agar MDR\/XDR-TB Colour Test (CT), a <jats:italic>Mycobacterium tuberculosis<\/jats:italic> complex (MTBC) detection and direct drug susceptibility testing (DST) method with routine sputum,\n bronchoalveolar lavage and pleural fluid specimen.&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;METHODS&lt;\/title&gt;In a prospective study, the time and rate of MTBC detection were compared between CT, L\u00f6wenstein-Jensen, and MGIT media. Times until DST result, sensitivities, and specificities\n were evaluated between CT and MGIT 960 indirect DST.&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;RESULTS&lt;\/title&gt;The cultivation of 177 pulmonary specimens resulted in 83 MTBC-positive cultures. The sensitivity of CT for MTBC detection was 81.3% with a median time of 20 days compared to\n 13 days and 93.5% for MGIT. The sensitivity of CT for DST results was 100% for isoniazid and levofloxacin and 94.7% for rifampicin. The specificities for isoniazid and rifampicin DST were 97.3% and 98.0% for levofloxacin. The median time until a DST result was significantly shorter with CT\n than the BACTEC MGIT 960 system, 20 and 27 days, respectively, independent of the specimen type used.&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;CONCLUSIONS&lt;\/title&gt;The CT is a highly accurate and fast initial diagnostic test for high-incidence settings and could also be used as a first\n culture and direct DST in peripheral settings.&lt;\/sec&gt;<\/jats:p>","DOI":"10.5588\/ijtld.23.0536","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T04:35:59Z","timestamp":1721882159000},"page":"387-394","source":"Crossref","is-referenced-by-count":0,"title":["Prospective evaluation of thin-layer agar colour test in routine diagnosis of multidrug-resistant TB"],"prefix":"10.5588","volume":"28","author":[{"given":"K.","family":"Klaos","sequence":"first","affiliation":[{"name":"Department of Mycobacteriology, United Laboratories, Tartu University Hospital, Tartu, Estonia;, Department of Pulmonology, University of Tartu, Tartu, Estonia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Agejeva","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology, United Laboratories, Tartu University Hospital, Tartu, Estonia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Hurt","sequence":"additional","affiliation":[{"name":"Lung Clinic, Tartu University Hospital, Tartu, Estonia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Kummik","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology, United Laboratories, Tartu University Hospital, Tartu, Estonia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kurve","sequence":"additional","affiliation":[{"name":"North Estonia Medical Centre, Centre of Pulmonology, Tallinn, Estonia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Nirk","sequence":"additional","affiliation":[{"name":"North Estonia Medical Centre, Centre of Pulmonology, Tallinn, Estonia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Pehme","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, University of Tartu, Tartu, Estonia;, Lung Clinic, Tartu University Hospital, Tartu, Estonia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Remets","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Diagnostics, Laboratory, North Estonia Medical Centre, Tallinn, Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Sasi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Diagnostics, Laboratory, North Estonia Medical Centre, Tallinn, Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Tann","sequence":"additional","affiliation":[{"name":"Department of Mycobacteriology, United Laboratories, Tartu University Hospital, Tartu, Estonia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Altraja","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, University of Tartu, Tartu, Estonia;, Lung Clinic, Tartu University Hospital, Tartu, Estonia;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3554","container-title":["The International Journal of Tuberculosis and Lung Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/iuatld\/ijtld\/2024\/00000028\/00000008\/art00005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T04:36:09Z","timestamp":1721882169000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.5588\/ijtld.23.0536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,1]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8,1]]}},"alternative-id":["1027-3719(20240801)28:8L.387;1-"],"URL":"https:\/\/doi.org\/10.5588\/ijtld.23.0536","relation":{},"ISSN":["1027-3719"],"issn-type":[{"value":"1027-3719","type":"print"}],"subject":[],"published":{"date-parts":[[2024,8,1]]}}}