{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T18:54:07Z","timestamp":1762109647714,"version":"build-2065373602"},"reference-count":0,"publisher":"International Union Against Tuberculosis and Lung Disease","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["int j tuberc lung dis"],"published-print":{"date-parts":[[2024,10,1]]},"abstract":"<sec><title>BACKGROUND<\/title>Detection of Mycobacterium tuberculosis<\/jats:italic> (MTB) in bioaerosols derived from patients with active pulmonary TB is a potential alternative diagnostic method for patients with presumed TB who cannot expectorate sputum.<\/sec><sec><title>OBJECTIVE<\/title>To\n assess the efficacy of a bioaerosol particle collection method to capture MTB and diagnose TB.<\/sec><sec><title>METHODS<\/title>A mask-like filter holder (3D mask) with a water-soluble gelatine filter (GF) and one containing a water-insoluble polypropylene filter\n (PPF) were prepared. Eligible patients wore the 3D mask with GF or PPF within 3 days of starting anti-TB drugs. The GF and PPF filters were collected after 2 and 8 h. DNA was extracted from the filter samples and tested using loop-mediated isothermal amplification (LAMP).<\/sec><sec><title>RESULTS<\/title>Filter\n samples were collected from 57 and 20 patients with and without active pulmonary TB, respectively. The GF and PPF sensitivity was 76.2% and 83.3%, respectively. The specificity of both methods was 100%. Of the 57 patients diagnosed with non-expectorated sputum samples, including suction phlegm,\n gastric lavage, and bronchial lavage fluid, 55.6% and 50.0% were positive by GF and PPF, respectively.<\/sec><sec><title>CONCLUSION<\/title>We present a 3D mask filter sampling method for exhaled bioaerosol particles that can be used in clinical practice to diagnose\n patients with presumed TB.<\/sec><\/jats:p>","DOI":"10.5588\/ijtld.24.0190","type":"journal-article","created":{"date-parts":[[2024,9,28]],"date-time":"2024-09-28T00:30:57Z","timestamp":1727483457000},"page":"476-481","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of testing face-mask filter samples with LAMP shows high rates of detection in pulmonary TB"],"prefix":"10.5588","volume":"28","author":[{"given":"T.","family":"Kodama","sequence":"first","affiliation":[{"name":"Respiratory Disease Center, Fukujuji Hospital, Japan Anti-Tuberculosis Association (JATA), Tokyo, Japan;, Department of Basic Mycobacteriosis, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Chikamatsu","sequence":"additional","affiliation":[{"name":"Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, JATA, Kiyose, Tokyo, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Kamada","sequence":"additional","affiliation":[{"name":"Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, JATA, Kiyose, Tokyo, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Fukujuji Hospital, JATA, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Morishige","sequence":"additional","affiliation":[{"name":"Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, JATA, Kiyose, Tokyo, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Igarashi","sequence":"additional","affiliation":[{"name":"Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, JATA, Kiyose, Tokyo, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Osugi","sequence":"additional","affiliation":[{"name":"Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, JATA, Kiyose, Tokyo, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Aono","sequence":"additional","affiliation":[{"name":"Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, JATA, Kiyose, Tokyo, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Murase","sequence":"additional","affiliation":[{"name":"Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, JATA, Kiyose, Tokyo, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Okumura","sequence":"additional","affiliation":[{"name":"Respiratory Disease Center, Fukujuji Hospital, Japan Anti-Tuberculosis Association (JATA), Tokyo, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Yoshiyama","sequence":"additional","affiliation":[{"name":"Respiratory Disease Center, Fukujuji Hospital, Japan Anti-Tuberculosis Association (JATA), Tokyo, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Takaki","sequence":"additional","affiliation":[{"name":"Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, JATA, Kiyose, Tokyo, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Mitarai","sequence":"additional","affiliation":[{"name":"Department of Basic Mycobacteriosis, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;, Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, JATA, Kiyose, Tokyo, Japan;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3554","container-title":["The International Journal of Tuberculosis and Lung Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/iuatld\/ijtld\/2024\/00000028\/00000010\/art00002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T04:35:19Z","timestamp":1757997319000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.5588\/ijtld.24.0190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,1]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10,1]]}},"alternative-id":["1027-3719(20241001)28:10L.476;1-"],"URL":"https:\/\/doi.org\/10.5588\/ijtld.24.0190","relation":{},"ISSN":["1027-3719"],"issn-type":[{"type":"print","value":"1027-3719"}],"subject":[],"published":{"date-parts":[[2024,10,1]]}}}