{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:21:27Z","timestamp":1790940087769,"version":"4.1.0"},"reference-count":0,"publisher":"International Union Against Tuberculosis and Lung Disease","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["int j tuberc lung dis"],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:p>&lt;sec&gt;&lt;title&gt;BACKGROUND&lt;\/title&gt;Existing TB diagnostic tests rely on sputum samples, which can be difficult to collect from all patients. This study examines plasma <jats:italic>Mycobacterium tuberculosis<\/jats:italic> cell-free DNA (Mtb cfDNA) based quantitative PCR (qPCR) assay for\n the diagnosis of pulmonary TB (PTB).&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;METHODS&lt;\/title&gt;The qPCR assay targeted insertion sequence (IS<jats:italic>6110<\/jats:italic>), <jats:italic>cyp141,<\/jats:italic> and <jats:italic>dev<\/jats:italic>R genes on plasma samples from 106 PTB patients and 60 controls. Sensitivity was calculated using the\n Xpert<jats:sup>\u00ae<\/jats:sup> MTB\/RIF test, culture, and clinical diagnosis for the PTB group, while specificity was determined based on results from controls.&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;RESULTS&lt;\/title&gt;Among PTB cases, 92 (86.8%) were bacteriologically confirmed, with the remaining\n 14 (13.2%) diagnosed clinically. The sensitivity of the plasma Mtb cfDNA assay, considering all three genes, was 71.7% (95% CI 62.6\u201371.7) for all PTB cases, with higher sensitivity in bacteriologically confirmed cases (78.3%) than in clinically diagnosed cases (28.6%). The combined specificity\n was 91.7%. The combination of IS<jats:italic>6110<\/jats:italic> and <jats:italic>cyp141<\/jats:italic> targeted qPCR demonstrated a sensitivity of 70.8%, and IS<jats:italic>6110<\/jats:italic> and <jats:italic>dev<\/jats:italic>R showed a sensitivity of 69.8%. However, <jats:italic>dev<\/jats:italic>R and <jats:italic>cyp141<\/jats:italic> resulted in a lower sensitivity of 63.2%. IS<jats:italic>6110<\/jats:italic> and <jats:italic>cyp141<\/jats:italic>\n had sensitivities of respectively 59.4% and 60.4%, while <jats:italic>dev<\/jats:italic>R had 53.8%.&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;CONCLUSION&lt;\/title&gt;Targeting multiple genes for plasma Mtb cfDNA-based TB diagnosis improves sensitivity and could be an important addition to current sputum-based\n diagnostic approaches.&lt;\/sec&gt;<\/jats:p>","DOI":"10.5588\/ijtld.24.0353","type":"journal-article","created":{"date-parts":[[2025,1,4]],"date-time":"2025-01-04T04:32:27Z","timestamp":1735965147000},"page":"13-19","source":"Crossref","is-referenced-by-count":4,"title":["Multigene <i>Mycobacterium tuberculosis<\/i> cell-free DNA assay"],"prefix":"10.5588","volume":"29","author":[{"given":"S.","family":"Ayalew","sequence":"first","affiliation":[{"name":"Armauer Hansen Research Institute, Addis Ababa, Ethiopia;, Department of Biology, College of Natural and Computational Sciences, Arba Minch University, Arba Minch, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Wegayehu","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Natural and Computational Sciences, Arba Minch University, Arba Minch, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Wondale","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Natural and Computational Sciences, Arba Minch University, Arba Minch, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.H.","family":"Alemayehu","sequence":"additional","affiliation":[{"name":"Armauer Hansen Research Institute, Addis Ababa, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Kebede","sequence":"additional","affiliation":[{"name":"Armauer Hansen Research Institute, Addis Ababa, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Osman","sequence":"additional","affiliation":[{"name":"Armauer Hansen Research Institute, Addis Ababa, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Niway","sequence":"additional","affiliation":[{"name":"Armauer Hansen Research Institute, Addis Ababa, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Piantadosi","sequence":"additional","affiliation":[{"name":"Emory University School of Medicine, Department of Pathology and Department of Medicine, Division of Infectious Diseases, Atlanta, GA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Mihret","sequence":"additional","affiliation":[{"name":"Armauer Hansen Research Institute, Addis Ababa, Ethiopia;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3554","container-title":["The International Journal of Tuberculosis and Lung Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/iuatld\/ijtld\/2025\/00000029\/00000001\/art00003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,4]],"date-time":"2025-01-04T04:32:35Z","timestamp":1735965155000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.5588\/ijtld.24.0353"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1,1]]}},"alternative-id":["1027-3719(20250101)29:1L.13;1-"],"URL":"https:\/\/doi.org\/10.5588\/ijtld.24.0353","relation":{},"ISSN":["1027-3719"],"issn-type":[{"value":"1027-3719","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1,1]]}}}