{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T12:47:23Z","timestamp":1783687643523,"version":"3.55.0"},"reference-count":0,"publisher":"International Union Against Tuberculosis and Lung Disease","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["int j tuberc lung dis"],"published-print":{"date-parts":[[2025,2,1]]},"abstract":"<jats:p>&lt;sec&gt;&lt;title&gt;OBJECTIVE&lt;\/title&gt;To use molecular techniques to assess the prevalence of <jats:italic>M. bovis<\/jats:italic> and <jats:italic>M. tuberculosis<\/jats:italic> in tuberculin-positive dairy cattle and to identify the risk factors for TB in these animals.&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;METHODS&lt;\/title&gt;A\n cross-sectional study was conducted from 2018 to 2020 across Mymensingh, Sirajgonj and Dhaka Districts in Bangladesh. The single intradermal comparative cervical tuberculin test was administered to 1,580 cattle suspected of having bovine TB using both avian and bovine purified protein derivative.\n Milk and lung tissue samples from positive animals were examined using polymerase chain reaction (PCR) to detect the causative agents of TB. Multivariable logistic regression model identified risk factors, and Sanger\u2019s dideoxy sequencing method was used for the phylogenetic analysis\n of PCR amplicons.&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;RESULTS&lt;\/title&gt;Simplex PCR identified <jats:italic>Mycobacterium tuberculosis<\/jats:italic> complex in 12.6% of samples. Multiplex PCR detected <jats:italic>M. bovis<\/jats:italic> in 6.3% and <jats:italic>M. tuberculosis<\/jats:italic> in 3.1% of the samples. Phylogenetic analysis of\n 12 IS<jats:italic>6110<\/jats:italic> gene sequences (8 <jats:italic>M. bovis<\/jats:italic>, 4 <jats:italic>M. tuberculosis<\/jats:italic>) confirmed alignment with human isolates from Bangladesh.&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;CONCLUSION&lt;\/title&gt;The study suggests potential reverse zoonotic transmission of <jats:italic>M. tuberculosis<\/jats:italic>. Further\n research is needed to understand the implications and assess TB transmission between humans and cattle in Bangladesh. The findings highlight the need for a comprehensive One Health approach.&lt;\/sec&gt;<\/jats:p>","DOI":"10.5588\/ijtld.24.0368","type":"journal-article","created":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T04:53:02Z","timestamp":1738385582000},"page":"80-87","source":"Crossref","is-referenced-by-count":1,"title":["Reversible zoonotic transmission of TB from humans to dairy cattle"],"prefix":"10.5588","volume":"29","author":[{"given":"M.N.","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh;, Department of Livestock Services, Dhaka, Bangladesh;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.K.","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh;, Department of Community Medicine, Mymensingh Medical College, Mymensingh, Bangladesh;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.S.","family":"Nahar","sequence":"additional","affiliation":[{"name":"Department of Animal Husbandry and Veterinary Medicine, University of Rajshahi, Rajshahi, Bangladesh."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.F.R.","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.A.","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.K.M.A.","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.M.","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3554","container-title":["The International Journal of Tuberculosis and Lung Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/iuatld\/ijtld\/2025\/00000029\/00000002\/art00006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T04:53:02Z","timestamp":1738385582000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.5588\/ijtld.24.0368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2,1]]}},"alternative-id":["1027-3719(20250201)29:2L.80;1-"],"URL":"https:\/\/doi.org\/10.5588\/ijtld.24.0368","relation":{},"ISSN":["1027-3719"],"issn-type":[{"value":"1027-3719","type":"print"}],"subject":[],"published":{"date-parts":[[2025,2,1]]}}}