{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T05:49:23Z","timestamp":1782884963519,"version":"3.54.5"},"reference-count":0,"publisher":"International Union Against Tuberculosis and Lung Disease","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["int j tuberc lung dis"],"published-print":{"date-parts":[[2026,6,29]]},"abstract":"<jats:p>&lt;sec&gt;&lt;title&gt;BACKGROUND&lt;\/title&gt;TB remains a major public health concern in Baja California, Mexico, where access to molecular diagnostics is limited.&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;METHODS&lt;\/title&gt;We evaluated the diagnostic performance of a fluorescent\n dye\u2013based real-time quantitative PCR (SYBR-qPCR) assay targeting the IS6110 sequence for the detection of pulmonary TB (PTB). This was a cross-sectional diagnostic accuracy study conducted using sputum samples from 113 adults with clinical suspicion of PTB. Samples were analysed by acid-fast\n bacilli (AFB) smear microscopy, L\u00f6wenstein\u2013Jensen culture as the reference standard, and SYBR-qPCR. Diagnostic performance was assessed using sensitivity, specificity, predictive values, and likelihood ratios. Receiver operating characteristic analysis was used to determine the\n optimal cycle threshold (Ct) cut-off.&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;RESULTS&lt;\/title&gt;SYBR-qPCR showed a sensitivity of 98.3% and a specificity of 92.5%. Sensitivity remained high among HIV-positive participants (96.6%), although specificity was lower (78.6%). In HIV-negative\n participants, both sensitivity and specificity exceeded 97%. The combination of SYBR-qPCR and AFB smear microscopy improved sensitivity (100%), and mean Ct values did not differ by HIV status.&lt;\/sec&gt;&lt;sec&gt;&lt;title&gt;CONCLUSION&lt;\/title&gt;SYBR-qPCR demonstrated consistent\n diagnostic performance across HIV strata, supporting its feasibility as a low-cost molecular tool for TB detection in resource-limited settings.&lt;\/sec&gt;<\/jats:p>","DOI":"10.5588\/ijtld.25.0693","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T04:33:48Z","timestamp":1782794028000},"page":"309-315","source":"Crossref","is-referenced-by-count":0,"title":["Accuracy of a fluorescent dye-qPCR for diagnosis of pulmonary TB in HIV-associated adults"],"prefix":"10.5588","volume":"30","author":[{"given":"J.H.","family":"Reyna-Verdugo","sequence":"first","affiliation":[{"name":"Centro Universitario de Educaci\u00f3n en la Salud (CUES), Universidad Aut\u00f3noma de Baja California (UABC), Tijuana, Mexico;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.R.","family":"Ch\u00e1vez-M\u00e9ndez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias de la Salud (FACISALUD), Universidad Aut\u00f3noma de Baja California (UABC), Tijuana, Mexico;, Albergue Las Memorias A.C., Tijuana, Mexico;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.M.","family":"Cornejo-Bravo","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas e Ingenier\u00eda (FCQI), Universidad Aut\u00f3noma de Baja California (UABC), Tijuana, Mexico;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Isa\u00edas de Le\u00f3n-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Facultad de Odontolog\u00eda Tijuana, Universidad Aut\u00f3noma de Baja California (UABC), Tijuana, Mexico;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.A.","family":"Reyes-Villegas","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas e Ingenier\u00eda (FCQI), Universidad Aut\u00f3noma de Baja California (UABC), Tijuana, Mexico;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Serrano-Medina","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas e Ingenier\u00eda (FCQI), Universidad Aut\u00f3noma de Baja California (UABC), Tijuana, Mexico;, Facultad de Medicina y Psicolog\u00eda, Universidad Aut\u00f3noma de Baja California (UABC), Tijuana,\n Mexico."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.A.","family":"Castillo-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias de la Salud (FACISALUD), Universidad Aut\u00f3noma de Baja California (UABC), Tijuana, Mexico;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3554","container-title":["The International Journal of Tuberculosis and Lung Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/iuatld\/ijtld\/2026\/00000030\/00000007\/art00003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T04:32:55Z","timestamp":1782880375000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.5588\/ijtld.25.0693"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,29]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,6,29]]}},"alternative-id":["1027-3719(20260629)30:7L.309;1-"],"URL":"https:\/\/doi.org\/10.5588\/ijtld.25.0693","relation":{},"ISSN":["1027-3719"],"issn-type":[{"value":"1027-3719","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6,29]]}}}