{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T02:02:16Z","timestamp":1784167336411,"version":"3.55.0"},"reference-count":73,"publisher":"International Association of Physical Chemists (IAPC)","license":[{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100015690","name":"Universitas Padjadjaran","doi-asserted-by":"publisher","award":["1957\/UN6.3.1\/PT.00\/2024"],"award-info":[{"award-number":["1957\/UN6.3.1\/PT.00\/2024"]}],"id":[{"id":"10.13039\/501100015690","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ADMET DMPK"],"abstract":"<jats:p>Background and purpose: Serological assays are essential for evaluating immune responses, including donor screening, vaccine efficacy, and antibody persistence. However, conventional methods are time-consuming and require centralized laboratories. This study aimed to develop a sensitive and rapid electrochemical immunosensor based on a cerium oxide-gold nanocomposite (CeO\u2082-Au) modified screen-printed carbon electrode (SPCE) for the detection of anti-SARS-CoV-2 IgG, while elucidating the underlying electrochemical sensing mechanism. Experimental approach: CeO\u2082-Au nanocomposites were synthesized and characterized using UV-Vis, SEM, TEM-EDX, and FTIR. The immuno\u00adsensor was fabricated by immobilizing SARS-CoV-2 Spike receptor-binding domain (RBD) onto the modified SPCE. Electrochemical responses were evaluated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) with K\u2083[Fe(CN)\u2086] as a redox probe. Key results: The CeO\u2082-Au nanocomposite enhanced electron transfer and provided a high surface area for biomolecule immobilization. The sensing mechanism is governed by modulation of interfacial electron transfer: binding of IgG to immobilized RBD forms an insulating immunocomplex layer, increasing charge transfer resistance and suppressing faradaic current. The sensor exhibited a wide linear range of 0.01 to 103 ng mL-1, a low detection limit of 2.475 pg mL-1, good stability, and reliable 97.3 to 108.56 % recovery in serum samples. Conclusion: This immunosensor proposed as a sensitive and reliable platform for IgG detection. The study advances understanding of signal transduction mechanisms in nanocomposite-based immunosensors and highlights their potential for rapid serological diagnostics. However, broader clinical validation and selectivity against complex interferents remain necessary.<\/jats:p>","DOI":"10.5599\/admet.3182","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T08:37:22Z","timestamp":1775810242000},"source":"Crossref","is-referenced-by-count":1,"title":["High-sensitivity electrochemical immunosensor for anti-SARS-CoV-2 IgG detection using screen-printed carbon\/cerium oxide-gold electrode"],"prefix":"10.5599","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7165-033X","authenticated-orcid":false,"given":"Melania Janisha","family":"Devi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6291-2465","authenticated-orcid":false,"given":"Ratu Shifa","family":"Syafira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3659-4774","authenticated-orcid":false,"given":"Shabarni","family":"Gaffar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9938-2931","authenticated-orcid":false,"given":"Irkham","family":"Irkham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7057-4358","authenticated-orcid":false,"given":"Yasuaki","family":"Einaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1463-6352","authenticated-orcid":false,"given":"Yeni Wahyuni","family":"Hartati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3564","published-online":{"date-parts":[[2026,4,10]]},"reference":[{"key":"173144","unstructured":"[1] Statement on the fifteenth meeting of the IHR (2005) Emergency Committee on the COVID-19 pandemic, World Health Organization. https:\/\/www.who.int\/news\/item\/05-05-2023-statement-on-the-fifteenth-meeting-of-the-international-health-regulations-(2005)-emergency-committee-regarding-the-coronavirus-disease-(covid-19)-pandemic (accessed November 23, 2023)."},{"key":"173145","doi-asserted-by":"crossref","unstructured":"[2] M.J. 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