{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T15:04:35Z","timestamp":1780067075595,"version":"3.54.0"},"reference-count":14,"publisher":"Polish Society of Microbiologists","issue":"2","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The aim of our studies was to invent a reliable method for detection of the bactericidal activity of disinfectants against\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    in suspension (\n                    <jats:italic>in vitro<\/jats:italic>\n                    ) and in cell line cultures (\n                    <jats:italic>in vivo<\/jats:italic>\n                    ). In the suspension method, 0.01% octenidine at 20\u00b0C and 35\u00b0C was bactericidal to\n                    <jats:italic>Borrelia afzeli<\/jats:italic>\n                    ;\n                    <jats:italic>Borrelia garini<\/jats:italic>\n                    ,\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    sensu stricto after 5 minutes treatment. Increase of the temperature to 35\u00b0C speed up the bactericidal effect to 1 minute. The bactericidal action of octenidine towards\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    spirochetes growing in fibroblasts was less effective and needed a longer time to kill them than in the suspension.\n                  <\/jats:p>","DOI":"10.5604\/01.3001.0010.7874","type":"journal-article","created":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T11:31:26Z","timestamp":1515497486000},"page":"259-263","source":"Crossref","is-referenced-by-count":3,"title":["Bactericidal Activity of Octenidine to Various Genospecies of\n                    <i>Borrelia burgdorferi<\/i>\n                    , Sensu Lato Spirochetes\n                    <i>in Vitro<\/i>\n                    and\n                    <i>in Vivo<\/i>"],"prefix":"10.33073","volume":"66","author":[{"given":"STANIS\u0141AWA","family":"TYLEWSKA-WIERZBANOWSKA","sequence":"first","affiliation":[{"name":"National Institute of Public Heath \u2013 National Institute of Hygiene , Laboratory of Rickettsia , Chlamydia and Spirochetes , Warsaw , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"URSZULA","family":"ROGUSKA","sequence":"additional","affiliation":[{"name":"National Institute of Public Heath \u2013 National Institute of Hygiene , Laboratory of Rickettsia , Chlamydia and Spirochetes , Warsaw , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GRA\u017bYNA","family":"LEWANDOWSKA","sequence":"additional","affiliation":[{"name":"National Institute of Public Heath \u2013 National Institute of Hygiene , Laboratory of Rickettsia , Chlamydia and Spirochetes , Warsaw , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TOMASZ","family":"CHMIELEWSKI","sequence":"additional","affiliation":[{"name":"National Institute of Public Heath \u2013 National Institute of Hygiene , Laboratory of Rickettsia , Chlamydia and Spirochetes , Warsaw , Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"18083","published-online":{"date-parts":[[2017,6,28]]},"reference":[{"key":"2026052913571644982_j_01.3001.0010.7874_ref_001","doi-asserted-by":"crossref","unstructured":"Chmielewski T. and S. Tylewska-Wierzbanowska. 2010. Interactions between Borrelia burgdorferi and mouse fibroblasts. Pol. J. Microbiol. 59: 157\u2013160.10.33073\/pjm-2010-024","DOI":"10.33073\/pjm-2010-024"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_002","doi-asserted-by":"crossref","unstructured":"Coburn J., G. Leong and G. Chaconas. 2013. Iluminating the role of the Borrelia burgdorferi adhesions. Trends Microbiol. 21: 372\u2013379.10.1016\/j.tim.2013.06.005","DOI":"10.1016\/j.tim.2013.06.005"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_003","doi-asserted-by":"crossref","unstructured":"Embers M.E., S.W. Barthold, J.T. Borda, L. Bowers, L. Doyle, E. Hodzic, M.B. Jacobs, N.R. Hasenkampf, D.S. Martin, S. Narasimhan and others. 2012. Persistence of Borrelia burgdorferi in rhesus macaques following antibiotic treatment of disseminated infection. PLoS One 7(1): e29914.10.1371\/journal.pone.0029914","DOI":"10.1371\/journal.pone.0029914"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_004","unstructured":"European Standard EN 1276: Chemical disinfectants and antiseptics \u2013 Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas \u2013 Test method and requirements. European Committee for Standardization, Brussels 2009."},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_005","doi-asserted-by":"crossref","unstructured":"Groshong A.M. and J.S. Blevins. 2014. Insights into biology of Borrelia burgdorferi gained through the application of molecular genetics. Adv. Appl. Microbiol. 86: 41\u2013143.10.1016\/B978-0-12-800262-9.00002-0","DOI":"10.1016\/B978-0-12-800262-9.00002-0"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_006","doi-asserted-by":"crossref","unstructured":"Harman M.W., S.M. Dunham-Ems, M.J. Caimano, A.A. Belperron, L.K. Bockenstedt, H.C. Fu, J.D. Radolf and C.W. Wolgemuth. 2012. Heterogeneous motility of the Lyme disease spirochete in gelatin mimics dissemination throught tissue. Proc. Natl. Acad. Sci. USA 109: 3059\u2013306410.1073\/pnas.1114362109","DOI":"10.1073\/pnas.1114362109"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_007","unstructured":"Harrison M.A. and I.F. Rae. 1997. General techniques of cell cultures, pp. 50, 72\u201373. Cambridge University Press.10.1017\/CBO9780511623226"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_008","doi-asserted-by":"crossref","unstructured":"Hodzic E., S. Feng, K. Holden, K.J. Freet and S.W. Barthold. 2008. Persistence of Borrelia burgdorferi following antibiotic treatment in mice. Antimicrob. Agents Chemother. 52: 1728\u20131736.10.1128\/AAC.01050-07","DOI":"10.1128\/AAC.01050-07"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_009","doi-asserted-by":"crossref","unstructured":"Motaleb A., J. Liu and R.M. Wooten. 2015. Spirocheta motility and chemotaxis in the natural enzotic cycle and development of Lyme disease. Curr. Op. Microbiol. 28: 106\u2013113.10.1016\/j.mib.2015.09.006","DOI":"10.1016\/j.mib.2015.09.006"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_010","doi-asserted-by":"crossref","unstructured":"Stanek G., G.P. Wormser, J. Gray and F. Strle. 2012. Lyme borreliosis. Lancet 4:461\u2013473.10.1016\/S0140-6736(11)60103-7","DOI":"10.1016\/S0140-6736(11)60103-7"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_011","doi-asserted-by":"crossref","unstructured":"Strabinger R.K., B.A. Summers, Y.F. Chang and M.J.G. Appel. 1997. Persistence of Borrelia burgdorferi in experimentally infected dogs after antibiotic treatment. J. Clin. Microbiol. 15: 111\u2013116.10.1128\/jcm.35.1.111-116.19972295218968890","DOI":"10.1128\/jcm.35.1.111-116.1997"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_012","doi-asserted-by":"crossref","unstructured":"Theophilus P.A.S., M.J. Victoria, K.M. Socarras, K.R. Filush, K. Gupta, D.F. Luecke and E. Sapi. 2015. Effectiveness of Stevia rebaudiana whole leaf extract against the various morphological forms of Borrelia burgdorferi in vitro. Eur. J. Microbiol. Immunol. 4: 268\u2013280.10.1556\/1886.2015.00031","DOI":"10.1556\/1886.2015.00031"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_013","doi-asserted-by":"crossref","unstructured":"Tylewska-Wierzbanowska S. and T. Chmielewski. 1997. The isolation of Borrelia burgdorferi spirochetes from clinical material in cell line cultures. Zentralbl. Bakteriol. 286: 363\u2013370.10.1016\/S0934-8840(97)80094-1","DOI":"10.1016\/S0934-8840(97)80094-1"},{"key":"2026052913571644982_j_01.3001.0010.7874_ref_014","doi-asserted-by":"crossref","unstructured":"Wolgemuth C.W. Flagellar motility of the pathogenic spirochetes. 2015. Semin. Cell Dev. Biol. 46: 104\u2013112.10.1016\/j.semcdb.2015.10.015499446926481969","DOI":"10.1016\/j.semcdb.2015.10.015"}],"container-title":["Polish Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pjm-microbiology.publisherspanel.com\/gicid\/pdf\/01.3001.0010.7874","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/reference-global.com\/pdf\/10.5604\/01.3001.0010.7874","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T14:16:46Z","timestamp":1780064206000},"score":1,"resource":{"primary":{"URL":"https:\/\/reference-global.com\/article\/10.5604\/01.3001.0010.7874"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,1]]},"references-count":14,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,6,28]]},"published-print":{"date-parts":[[2017,1,1]]}},"alternative-id":["10.5604\/01.3001.0010.7874"],"URL":"https:\/\/doi.org\/10.5604\/01.3001.0010.7874","relation":{},"ISSN":["2544-4646"],"issn-type":[{"value":"2544-4646","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,1]]}}}