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Five groups of female SD rats were intragastrically administered with 4 different concentrations of AlCl3 for 5 days a week for a total of 90 days. The aluminum concentration was determined via graphite furnace atomic absorption spectroscopy. Analysis of serum iron-kinetic profiles, blood cytometry outcomes, and blood smears of the blood samples. Scanning electron microscopy (SEM) and Raman spectroscopy were used to search for structural and ultrastructural changes, respectively. Blood aluminum concentration ranged 12.38-16.24 \u03bcg\/L with no significant difference between experimental treatments. At the AlCl3 concentration of 40 mg Al\/kg bw of rats\/day, the mean ferritin value in the serum iron kinetic profile was 29.81\u00b16.1 ng\/mL, and this value showed a significant difference between experimental treatments. Blood cytometry revealed that there were 6.45-7.11\u00d710&lt;sup&gt;6&lt;\/sup&gt; cells\/\u03bcL erythrocytes, 8.91-9.32\u00d710&lt;sup&gt;3&lt;\/sup&gt; cells\/\u03bcL leukocytes, and 477.2-736.3\u00d710&lt;sup&gt;3&lt;\/sup&gt; cells\/\u03bcL platelets along with a hemoglobin of 37.38-41.93 g\/dL and hematocrit level of 37.38-41.93%; the experimental treatments showed no significant differences. Erythrocyte structural analysis using SEM showed no differences between experimental treatments, whereas ultrastructural evaluation using Raman spectroscopy made it possible to identify the following bands: 741, 1123, 1350, 1578, and 1618 cm&lt;sup&gt;\u22121&lt;\/sup&gt;, which were respectively associated with the following vibrational modes and compounds: vibration of the tryptophan ring, asymmetric C-O-C stretching of glucose, C-H curve of tryptophan, C=C stretching of the heme group, and C-N stretching of the heme group, with no significant differences between experimental treatments. Therefore, AlCl3 administration does not induce ultrastructural changes in the erythrocyte membrane. This study revealed that serum ferritin concentration was the only parameter affected by AlCl3 exposure at 40 mg of Al\/kg bw of rats\/day.<\/jats:p>","DOI":"10.5620\/eaht.2022034","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T07:09:07Z","timestamp":1667459347000},"page":"e2022034","update-policy":"https:\/\/doi.org\/10.5620\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Hematologic evaluation of peripheral blood in Sprague Dawley rats by chronic exposure to aluminum chloride (AlCl3)"],"prefix":"10.5620","volume":"37","author":[{"given":"Alba Rita","family":"Valenzuela-Brise\u00f1o","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanette Guadalupe","family":"Arredondo-Damian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Rasc\u00f3n-Careaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2452-0057","authenticated-orcid":true,"given":"Humberto","family":"Astiazaran-Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6392-1013","authenticated-orcid":true,"given":"Agust\u00edn","family":"G\u00f3mez-\u00c1lvarez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reynaldo","family":"Esquivel-G\u00f3nzalez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1295-5168","authenticated-orcid":true,"given":"Roberto Carlos","family":"Carrillo-Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique","family":"\u00c1lvarez-Ramos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Canett-Romero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1618-2238","authenticated-orcid":true,"given":"Leticia","family":"Garc\u00eda-Rico","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6197-7083","authenticated-orcid":true,"given":"Alejandro Monserrat","family":"Garc\u00eda-Alegr\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3578","published-online":{"date-parts":[[2022,11,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2011.06.013"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(02)00077-2"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4854152"},{"key":"ref4","first-page":"48","volume-title":"A review on toxic effects of aluminium exposure on male reproductive system and probable mechanisms of toxicity","author":"Pandey","year":"2013"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s12291-012-0250-7"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2015.02.002"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.07.035"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-018-1352-8"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2017.11.007"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2018.01.003"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1177\/0748233712440140"},{"key":"ref12","first-page":"255","volume-title":"Effect of ethanolic leaf extract of Moringa oleifera on aluminum-induced anemia in white albino rats","author":"Osman","year":"2012"},{"key":"ref13","first-page":"6","volume-title":"Effect of aluminum on different parts of brainstem of old","author":"Manisha","year":"2013"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.18287\/1613-0073-2017-1900-64-68"},{"volume-title":"","key":"ref15"},{"volume-title":"","year":"2014","key":"ref16"},{"volume-title":"","year":"2009","key":"ref17"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gep003"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2009.03.004"},{"volume-title":"Sacrificio humanitario de los animales dom\u00e9sticos y silvestres. 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