{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T08:01:48Z","timestamp":1782892908115,"version":"3.54.5"},"reference-count":0,"publisher":"Academy of Sciences and Arts of Bosnia and Herzegovina","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ama"],"abstract":"<jats:p>&lt;p&gt;&lt;strong&gt;Objectives. &lt;\/strong&gt;&lt;em&gt;In silico &lt;\/em&gt;bioinformatical analysis suggested that the expression of two genes, &lt;em&gt;CCL5 &lt;\/em&gt;(C-C Motif Chemokine Receptor 5) and &lt;em&gt;ep300 &lt;\/em&gt;(Histone acetyltransferase p300), could be used as potential new biomarkers in differentiation between periapical granulomas and radicular cysts. Thus, we hypothesized that gene expression of &lt;em&gt;CCL5 &lt;\/em&gt;and &lt;em&gt;ep300 &lt;\/em&gt;in periapical lesions would classify the lesions as either granuloma or cyst.&lt;\/p&gt;&lt;p&gt;&lt;strong&gt;Materials. &lt;\/strong&gt;Patient samples (n=122) included 46 periapical granulomas, 38 radicular cysts and 38 healthy gingival samples as controls. Real-time PCR analysis of &lt;em&gt;CCL5 &lt;\/em&gt;and &lt;em&gt;ep300 &lt;\/em&gt;transcripts was compared to &lt;em&gt;SDHA &lt;\/em&gt;(Succinate dehydrogenase complex, subunit A) as the reference. Clinical parameters (e.g., intensity of inflammation and lesion size) were measured and correlated with &lt;em&gt;CCL5 &lt;\/em&gt;and &lt;em&gt;ep300 &lt;\/em&gt;expression. ROC (Receiver operating characteristic) and logistic regression analyses were used to establish the diagnostic character of \u0394Ct values.&lt;\/p&gt;&lt;p&gt;&lt;strong&gt;Results. &lt;\/strong&gt;Granulomas and radicular cysts had significantly higher expression of &lt;em&gt;CCL5 &lt;\/em&gt;and &lt;em&gt;ep300 &lt;\/em&gt;compared to controls (P&amp;lt;0.05). However, no differences were observed when comparing granulomas and radicular cysts. ROC analyses showed that &lt;em&gt;CCL5 &lt;\/em&gt;and &lt;em&gt;ep300 &lt;\/em&gt;have good diagnostic accuracy, but low accuracy for distinguishing between the lesions.&lt;\/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions. &lt;\/strong&gt;This study confirmed that expression of &lt;em&gt;CCL5 &lt;\/em&gt;and &lt;em&gt;ep300 &lt;\/em&gt;is relevant for the pathogenesis of periapical inflammatory lesions but cannot be used as a distinctive marker between these lesions.&lt;\/p&gt;<\/jats:p>","DOI":"10.5644\/ama2006-124.251","type":"journal-article","created":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T11:05:48Z","timestamp":1621249548000},"page":"129","source":"Crossref","is-referenced-by-count":7,"title":["Quantitative Analysis of CCL5 and ep300 in Periapical Inflammatory Lesions"],"prefix":"10.5644","volume":"48","author":[{"given":"Naida","family":"Hadziabdic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amina","family":"Kurtovic-Kozaric","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azra","family":"Frkatovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira","family":"Smajlovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariadne","family":"Letra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3666","published-online":{"date-parts":[[2019,10,24]]},"container-title":["Acta Medica Academica"],"original-title":[],"link":[{"URL":"http:\/\/www.ama.ba\/index.php\/ama\/article\/viewFile\/368\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T11:05:48Z","timestamp":1621249548000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.ama.ba\/index.php\/ama\/article\/view\/368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,24]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,10,24]]}},"URL":"https:\/\/doi.org\/10.5644\/ama2006-124.251","relation":{},"ISSN":["1840-2879","1840-1848"],"issn-type":[{"value":"1840-2879","type":"electronic"},{"value":"1840-1848","type":"print"}],"subject":[],"published":{"date-parts":[[2019,10,24]]}}}