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Impact of inflammation on male reproductive tract. J. Reprod. Infertil. 16, 123-129 (2015)."},{"key":"2","unstructured":"2) John, P. The role of signaling pathways of inflammation and oxidative stress in development of senescence and aging phenotypes in cardiovascular disease. Tissue Cell 78, 101876 (2022)."},{"key":"3","doi-asserted-by":"crossref","unstructured":"3) Agarwal, A.; Saleh, R.A.; Bedaiwy, M.A. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil. Steril. 79, 829-843 (2003).","DOI":"10.1016\/S0015-0282(02)04948-8"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4) Zhu, X.; Shi, D.; Li, X.; Gong, W.; Wu, F.; Guo, X. TLR signalling affects sperm mitochondrial function and motility via phosphatidylinositol3-kinase and glycogen synthase kinase-3 alpha. 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Olive (Olea europea L) leaf extract attenuates early diabetic neuropathic pain through prevention of high glucose-induced apoptosis: In vitro and in vivo studies. J. Ethnopharmacol. 136, 188-196 (2011).","DOI":"10.1016\/j.jep.2011.04.038"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14) Badr, A.M.; Attia, H.A.; Al-Rasheed, N. Oleuropein reverses repeated corticosterone-induced depressive-like behavior in mice: Evidence of modulating effect on biogenic amines. Sci. Rep. 10, 3336 (2020).","DOI":"10.1038\/s41598-020-60026-1"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15) Nyambe-Silavwe, H.; Pyner, A.; Oladele, E.; Gauer, J.S.; Stevens, Y.; Williamson, G. Nutritional implications of olives and sugar: attenuation of post-prandial glucose spikes in healthy volunteers by inhibition of sucrose hydrolysis and glucose transport by oleuropein. Eur. J. Nutr. 58, 1315-1330 (2019).","DOI":"10.1007\/s00394-018-1662-9"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Xing, C.; Xu, L.; Yao, Y. Beneficial role of oleuropein in sepsis-induced myocardial injury. Possible Involvement of GSK-3\u03b2\/NF-kB pathway. Acta Cir. Bras. 36, e360107 (2021).","DOI":"10.1590\/acb360107"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17) Andreadou, L.; Iliodromitis, E.K.; Mikros, E.; Constantinou, M.; Agalias, A. et al. The olive constituent oleuropein exhibits anti-ischemic, antioxidative and hypolipidemic effects in anesthetized rabbits. J. Nutr. 136, 2213-2219 (2006).","DOI":"10.1093\/jn\/136.8.2213"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18) Kucukgul, A.; Isgor, M.M.; Duzguner, V.; Atabay, M.N.; Kucukgul, A. Antioxidant effects of oleuropein on hydrogen peroxide-induced neuronal stress- An in vitro study. Antiinflamm. Antiallergy Agents Med. 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Signal. 11, 317-327 (2015).","DOI":"10.1007\/s12079-017-0391-9"},{"key":"40","doi-asserted-by":"crossref","unstructured":"40) Tripoli, E.; Giammanco, M.; Tabacchi, G.; Di Majo, D.; Giammanco, S.; La Guardia, M. The phenolic compounds of olive oil: Structure, biological activity and beneficial effects on human health. Nutr. Res. Rev. 18, 98-112 (2015).","DOI":"10.1079\/NRR200495"},{"key":"41","doi-asserted-by":"crossref","unstructured":"41) Hamden, K.; Allouche, N.; Damak, M.; Elfeki, A. Hypoglycemic and antioxidant effects of phenolic extracts and purified hydroxytyrosol from olive mill waste in vitro and in rats. ChemBiolInteract 180, 421-432 (2009).","DOI":"10.1016\/j.cbi.2009.04.002"},{"key":"42","unstructured":"42) Popovicci, C.; Ilonka, S.; Bartek, T. Evaluation de l\u2019activit\u00e9 antioxydant des compos\u00e9s ph\u00e9noliques par la r\u00e9activit\u00e9 avec le radical libre DPPH. Revue de g\u00e9nie industriel 4, 25-39 (2009)."},{"key":"43","doi-asserted-by":"crossref","unstructured":"43) Odiatou, E.M.; Skaltsounis, A.L.; Constantinou, A.I. Identification of the factors responsible for the in vitro pro-oxidant and cytotoxic activities of the olive polyphenols oleuropein and hydroxytyrosol. Cancer Lett. 330, 113-121 (2013).","DOI":"10.1016\/j.canlet.2012.11.035"},{"key":"44","doi-asserted-by":"crossref","unstructured":"44) Mazziotti, A.; Mazzotti, F.; Pantusa, M.; Sportelli, L.; Sindona, G. Pro-oxidant activity of oleuropein determined in vitro by electron spin resonance spin-trapping methodology. J. Agric. Food Chem. 54, 7444-7449 (2006).","DOI":"10.1021\/jf060982c"},{"key":"45","doi-asserted-by":"crossref","unstructured":"45) Nousis, L.; Doulias, P.T.; Aligiannis, N.; Bazios, D.; Agalias, A. et al. DNA protecting and genotoxic effects of olive oil related components in cells exposed to hydrogen peroxide. Free Radic. 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