{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T23:33:11Z","timestamp":1777937591119,"version":"3.51.4"},"reference-count":33,"publisher":"Japan Oil Chemists' Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Oleo Sci."],"published-print":{"date-parts":[[2026]]},"DOI":"10.5650\/jos.ess25248","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T22:15:29Z","timestamp":1777587329000},"page":"577-588","source":"Crossref","is-referenced-by-count":0,"title":["Ozonated Olive Oil Attenuates Hepatic Steatosis in Obese <i>db\/db<\/i> Mice: Evidence for Triacylglycerol-derived Ozonides as Key Bioactive Species"],"prefix":"10.5650","volume":"75","author":[{"given":"Yasunari","family":"Kato","sequence":"first","affiliation":[{"name":"Tsukishima Foods Industry Co. Ltd., 3-17-9, Higashi Kasai, Edogawa-ku, Tokyo, 134-8520, JAPAN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leon","family":"Kotsuji","sequence":"additional","affiliation":[{"name":"Department of Biological Resource Science, Saga University, 1 Honjo-machi, Saga, 840-8502, JAPAN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiharu","family":"Nagai","sequence":"additional","affiliation":[{"name":"School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1, Katakuramachi, Hachioji City, Tokyo, 192-0982, JAPAN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiko","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Tsukishima Foods Industry Co. Ltd., 3-17-9, Higashi Kasai, Edogawa-ku, Tokyo, 134-8520, JAPAN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Ishida","sequence":"additional","affiliation":[{"name":"Tsukishima Foods Industry Co. Ltd., 3-17-9, Higashi Kasai, Edogawa-ku, Tokyo, 134-8520, JAPAN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nao","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Biological Resource Science, Saga University, 1 Honjo-machi, Saga, 840-8502, JAPAN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teruyoshi","family":"Yanagita","sequence":"additional","affiliation":[{"name":"Department of Biological Resource Science, Saga University, 1 Honjo-machi, Saga, 840-8502, JAPAN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Nagao","sequence":"additional","affiliation":[{"name":"Department of Biological Resource Science, Saga University, 1 Honjo-machi, Saga, 840-8502, JAPAN"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3621","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1) Portincasa, P.; Khalil, M.; Mahdi, L.; Perniola, V.; Idone, V. et al. Metabolic dysfunction-associated steatotic liver disease: from pathogenesis to current therapeutic options. Int. J. Mol. Sci. 25, 5640 (2024). doi: 10.3390\/ijms25115640","DOI":"10.3390\/ijms25115640"},{"key":"2","doi-asserted-by":"publisher","unstructured":"2) Manolis, A.A.; Manolis, T.A.; Vouliotis, A.; Manolis, A.S. Metabolic dysfunction-associated steatotic liver disease and the cardiovascular system. Trends Cardiovasc. Med. 5, 258-265 (2025). doi:10.1016\/j.tcm.2025.01.001","DOI":"10.1016\/j.tcm.2025.01.001"},{"key":"3","doi-asserted-by":"publisher","unstructured":"3) Zahardis, J.; Petrucci, G.A. The oleic acid-ozone heterogeneous reaction system: products, kinetics, secondary chemistry, and atmospheric implications of a model system - a review. Atmos. Chem. Phys. 7, 1237-1274 (2007). doi: 10.5194\/acp-7-1237-2007","DOI":"10.5194\/acp-7-1237-2007"},{"key":"4","doi-asserted-by":"publisher","unstructured":"4) Sadowska, J.; Johansson, B.; Johannessen, E.; Friman, R.; Broniarz-Press, L. et al. Characterization of ozonated vegetable oils by spectroscopic and chromatographic methods. Chem. Phys. Lipids 151, 85-91 (2008). doi:10.1016\/j.chemphyslip.2007.10.004","DOI":"10.1016\/j.chemphyslip.2007.10.004"},{"key":"5","doi-asserted-by":"publisher","unstructured":"5) Curr\u00f2, M.; Russo, T.; Ferlazzo, N.; Caccamo, D.; Antonuccio, P. et al. Anti-inflammatory and tissue regenerative effects of topical treatment with ozonated olive oil\/vitamin E acetate in balanitis xerotica obliterans. Molecules 23, 645 (2018). doi:10.3390\/molecules23030645","DOI":"10.3390\/molecules23030645"},{"key":"6","doi-asserted-by":"publisher","unstructured":"6) Passidomo, F.; Pignatelli, F.; Addabbo, G.; Costagliola, C. Topical liposomal ozonated oil in complicated corneal disease: a report on three clinical cases. Int. Med. Case Rep. J. 14, 327-332 (2021). doi:10.2147\/IMCRJ.S311839","DOI":"10.2147\/IMCRJ.S311839"},{"key":"7","doi-asserted-by":"publisher","unstructured":"7) Xiao, W.R.; Wu, M.; Bi, X.R. Ozone oil promotes wound healing via increasing miR-21-5p-mediated inhibition of RASA1. Wound Repair Regen. 29, 406-416 (2021). doi: 10.1111\/wrr.12907","DOI":"10.1111\/wrr.12907"},{"key":"8","doi-asserted-by":"publisher","unstructured":"8) Bertuccio, M.P.; Rizzo, V.; Arena, S.; Trainito, A.; Montalto, A.S. et al. Ozoile reduces the LPS-induced inflammatory response in colonic epithelial cells and THP-1 monocytes. Curr. Issues Mol. Biol. 45, 1333-1348 (2023). doi: 10.3390\/cimb45020087","DOI":"10.3390\/cimb45020087"},{"key":"9","doi-asserted-by":"publisher","unstructured":"9) Priya, S.; Reche, A.; Sonar, P.R. Conventional frenectomy with topical ozonated oil application. Cureus 16, e55522 (2024). doi:10.7759\/cureus.55522","DOI":"10.7759\/cureus.55522"},{"key":"10","doi-asserted-by":"publisher","unstructured":"10) Slavinskien\u0117, G.; Grigonis, A.; Iva\u0161kien\u0117, M.; Sinkevi\u010dien\u0117, I.; Andrulevi\u010di\u016bt\u0117, V. et al. A comparative study of the chemical properties and antibacterial activity of four different ozonated oils for veterinary purposes. Vet. Sci. 11, 161 (2024). doi:10.3390\/vetsci11040161","DOI":"10.3390\/vetsci11040161"},{"key":"11","doi-asserted-by":"publisher","unstructured":"11) Kato, Y.; Sakoh, M.; Nagai, T.; Yoshida, A.; Ishida, H. et al. Ozonated olive oil alleviates hepatic steatosis in obese Zucker (fa\/fa) rats. J. Oleo Sci. 71, 599-607 (2022). doi:10.5650\/jos.ess21377","DOI":"10.5650\/jos.ess21377"},{"key":"12","doi-asserted-by":"publisher","unstructured":"12) Kato, Y.; Sakoh, M.; Nagai, T.; Yoshida, A.; Ishida, H. et al. Ozonated olive oil intake attenuates hepatic steatosis in obese db\/db mice. J. Oleo Sci. 73, 231-237 (2024). doi:10.5650\/jos.ess23193","DOI":"10.5650\/jos.ess23193"},{"key":"13","doi-asserted-by":"publisher","unstructured":"13) Jimenez-Lopez, C.; Carpena, M.; Louren\u00e7o-Lopes, C.; Gallardo-Gomez, M.; Lorenzo, J.M. et al. Bioactive compounds and quality of extra virgin olive oil. Foods 9, 1014 (2020). doi:10.3390\/foods9081014","DOI":"10.3390\/foods9081014"},{"key":"14","doi-asserted-by":"publisher","unstructured":"14) Kato, Y.; Odanaka, Y.; Nagai, T. Analysis of ozonolysis products of ethyl linoleate reacted in dichloromethane. Ozone Sci. Eng. 47, 117-130 (2024). doi:10.1080\/01919512.2024.2396142","DOI":"10.1080\/01919512.2024.2396142"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15) Folch, J.; Lees, M.; Sloane Stanley, G.H. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226, 497-509 (1957). doi:10.1016\/S0021-9258(18)64849-5","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Fletcher, M.J. A colorimetric method for estimating serum triglycerides. Clin. Chim. Acta 22, 393-397 (1968). doi:10.1016\/0009-8981(68)90041-7","DOI":"10.1016\/0009-8981(68)90041-7"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17) Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; Randall, R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275 (1951). doi: 10.1016\/S0021-9258(19)52451-6","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"18","doi-asserted-by":"publisher","unstructured":"18) Kelley, D.S.; Nelson, G.J.; Hunt, J.E. Effect of prior nutritional status on the activity of lipogenic enzymes in primary monolayer cultures of rat hepatocytes. Biochem. J. 235, 87-90 (1986). doi:10.1042\/bj2350087","DOI":"10.1042\/bj2350087"},{"key":"19","doi-asserted-by":"publisher","unstructured":"19) Markwell, M.A.; McGroarty, E.J.; Bieber, L.L.; Tolbert, N.E. The subcellular distribution of carnitine acyltransferases in mammalian liver and kidney. a new peroxisomal enzyme. J. Biol. Chem. 248, 3426-3432 (1973). doi: 10.1016\/S0021-9258(19)43946-X","DOI":"10.1016\/S0021-9258(19)43946-X"},{"key":"20","doi-asserted-by":"publisher","unstructured":"20) Yamada, M.; Kita, Y.; Kohira, T.; Yoshida, K.; Hamano, F. et al. A comprehensive quantification method for eicosanoids and related compounds by using liquid chromatography\/mass spectrometry with high speed continuous ionization polarity switching. J. Chromatogr. B 995-996, 74-84 (2015). doi: 10.1016\/j.jchromb.2015.05.015","DOI":"10.1016\/j.jchromb.2015.05.015"},{"key":"21","doi-asserted-by":"publisher","unstructured":"21) Lefere, S.; Tacke, F. Macrophages in obesity and non-alcoholic fatty liver disease: crosstalk with metabolism. JHEP Rep. 1, 30-43 (2019). doi: 10.1016\/j.jhepr.2019.02.004","DOI":"10.1016\/j.jhepr.2019.02.004"},{"key":"22","doi-asserted-by":"publisher","unstructured":"22) McArdle, M.A.; Finucane, O.M.; Connaughton, R.M.; McMorrow, A.M.; Roche, H.M. Mechanisms of obesity-induced inflammation and insulin resistance: insights into the emerging role of nutritional strategies. Front. Endocrinol. (Lausanne) 4, 52 (2013). doi:10.3389\/fendo.2013.00052","DOI":"10.3389\/fendo.2013.00052"},{"key":"23","doi-asserted-by":"publisher","unstructured":"23) Kim, H.M.; Lee, E.S.; Lee, B.R.; Yadav, D.; Kim, Y.M. et al. C-C chemokine receptor 2 inhibitor ameliorates hepatic steatosis by improving ER stress and inflammation in a type 2 diabetic mouse model. PLoS One 10, e0120711 (2015). doi: 10.1371\/journal.pone.0120711","DOI":"10.1371\/journal.pone.0120711"},{"key":"24","doi-asserted-by":"publisher","unstructured":"24) Xue, H.; Guo, H.; Li, Y.C.; Hao, Z.M. Heme oxygenase-1 induction by hemin protects liver cells from ischemia\/reperfusion injury in cirrhotic rats. World J. Gastroenterol. 13, 5384-5390 (2007). doi:10.3748\/wjg.v13.i40.5384","DOI":"10.3748\/wjg.v13.i40.5384"},{"key":"25","doi-asserted-by":"publisher","unstructured":"25) Cisterna, B.; Costanzo, M.; Nodari, A.; Gali\u00e8, M.; Zanzoni, S. et al. Ozone activates the Nrf2 pathway and improves preservation of explanted adipose tissue in vitro. Antioxidants (Basel) 9, 989 (2020). doi:10.3390\/antiox9100989","DOI":"10.3390\/antiox9100989"},{"key":"26","doi-asserted-by":"publisher","unstructured":"26) Li, N.; Hao, L.; Li, S.; Deng, J.; Yu, F. et al. The NRF-2\/HO-1 signaling pathway: a promising therapeutic target for metabolic dysfunction-associated steatotic liver disease. J. Inflamm. Res. 17, 8061-8083 (2024). doi: 10.2147\/JIR.S490418","DOI":"10.2147\/JIR.S490418"},{"key":"27","doi-asserted-by":"publisher","unstructured":"27) Kato, Y.; Manabe, Y.; Inoue, N.; Nagao, K.; Nagai, T. et al. Anti-inflammatory function of ozonated ethyl ester of unsaturated fatty acids. J. Oleo Sci. 74, 495-501 (2025). doi:10.5650\/jos.ess25040","DOI":"10.5650\/jos.ess25040"},{"key":"28","doi-asserted-by":"publisher","unstructured":"28) Parchem, K.; Letsiou, S.; Petan, T.; Oskolkova, O.; Medina, I. et al. Oxylipin profiling for clinical research: current status and future perspectives. Prog. Lipid Res. 95, 101276 (2024). doi:10.1016\/j.plipres.2024.101276","DOI":"10.1016\/j.plipres.2024.101276"},{"key":"29","doi-asserted-by":"publisher","unstructured":"29) Mazi, T.A.; Borkowski, K.; Fiehn, O.; Bowlus, C.L.; Sarkar, S. et al. Plasma oxylipin profile discriminates ethnicities in subjects with non-alcoholic steatohepatitis: an exploratory analysis. Metabolites 12, 192 (2022). doi:10.3390\/metabo12020192","DOI":"10.3390\/metabo12020192"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30) Nagatake, T.; Kunisawa, J. Emerging roles of metabolites of \u03c93 and \u03c96 essential fatty acids in the control of intestinal inflammation. Int. Immunol. 31, 569-577 (2019). doi:10.1093\/intimm\/dxy086","DOI":"10.1093\/intimm\/dxy086"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31) Bazwinsky-Wutschke, I.; Zipprich, A.; Dehghani, F. Endocannabinoid system in hepatic glucose metabolism, fatty liver disease, and cirrhosis. Int. J. Mol. Sci. 20, 2516 (2019). doi:10.3390\/ijms20102516","DOI":"10.3390\/ijms20102516"},{"key":"32","doi-asserted-by":"publisher","unstructured":"32) Osei-Hyiaman, D.; DePetrillo, M.; Pacher, P.; Liu, J.; Radaeva, S. et al. Endocannabinoid activation at hepatic CB1 receptors stimulates fatty acid synthesis and contributes to diet-induced obesity. J. Clin. Invest. 115, 1298-1305 (2005). doi:10.1172\/JCI23057","DOI":"10.1172\/JCI23057"},{"key":"33","doi-asserted-by":"publisher","unstructured":"33) Fu, J.; Gaetani, S.; Oveisi, F.; Lo Verme, J.; Serrano, A. et al. Oleylethanolamide regulates feeding and body weight through activation of the nuclear receptor PPAR-alpha. Nature 425, 90-93 (2003). doi:10.1038\/nature01921","DOI":"10.1038\/nature01921"}],"container-title":["Journal of Oleo Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jos\/75\/5\/75_jos.ess25248\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T04:10:40Z","timestamp":1777695040000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jos\/75\/5\/75_jos.ess25248\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":33,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.5650\/jos.ess25248","relation":{},"ISSN":["1345-8957","1347-3352"],"issn-type":[{"value":"1345-8957","type":"print"},{"value":"1347-3352","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"jos.ess25248"}}