{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T05:01:29Z","timestamp":1780722089713,"version":"3.54.1"},"reference-count":31,"publisher":"Japan Oil Chemists' Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Oleo Sci."],"published-print":{"date-parts":[[2026]]},"DOI":"10.5650\/jos.ess26022","type":"journal-article","created":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T22:13:38Z","timestamp":1780265618000},"page":"703-711","source":"Crossref","is-referenced-by-count":0,"title":["Domain analysis and engineering of multifunctional \u03b2-carotene synthase, CrtIBY, for lycopene and phytoene production"],"prefix":"10.5650","volume":"75","author":[{"given":"Kenshi","family":"Watanabe","sequence":"first","affiliation":[{"name":"11-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, JAPAN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ami","family":"Oda","sequence":"additional","affiliation":[{"name":"11-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, JAPAN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Nagano","sequence":"additional","affiliation":[{"name":"11-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, JAPAN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryota","family":"Sato","sequence":"additional","affiliation":[{"name":"11-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, JAPAN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Hashima","sequence":"additional","affiliation":[{"name":"11-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, JAPAN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsunehiro","family":"Aki","sequence":"additional","affiliation":[{"name":"11-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, JAPAN"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3621","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1) Harari, A., Harats, D., Marko, D., Cohen, H., Barshack, I., et al. A 9-cis \u03b2-carotene-enriched diet inhibits atherogenesis and fatty liver formation in LDL receptor knockout mice. J. Nutr., 138, 1923-1930 (2008).","DOI":"10.1093\/jn\/138.10.1923"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2) Jyonouchi, H., Sun, S., Iijima, K., Gross, M.D. Antitumor activity of astaxanthin and its mode of action. Nutr. Cancer, 36, 59-65 (2000).","DOI":"10.1207\/S15327914NC3601_9"},{"key":"3","doi-asserted-by":"publisher","unstructured":"3) Tanaka, T., Morishita, Y., Suzui, M., Kojima, T., Okumura, A., et al. Chemoprevention of mouse urinary bladder carcinogenesis by the naturally occurring carotenoid astaxanthin. Carcinogenesis, 15, 15-19 (1994).","DOI":"10.1093\/carcin\/15.1.15"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4) Sandmann, G. Carotenoid biosynthesis in microorganisms and plants. Eur. J. Biochem., 223, 7-24 (1994).","DOI":"10.1111\/j.1432-1033.1994.tb18961.x"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5) Shaish, A., Harari, A., Kamari, Y., Soudant, E., Harats, D., et al. A carotenoid algal preparation containing phytoene and phytofluene inhibited LDL oxidation in vitro. Plant Foods Hum. Nutr., 63, 83-86 (2008).","DOI":"10.1007\/s11130-008-0075-y"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6) Mapelli-Brahm, P., Stinco, C.M., Rodrigo, M.J., Zacar\u00edas, L., Mel\u00e9ndez-Mart\u00ednez, A.J. Impact of thermal treatments on the bioaccessibility of phytoene and phytofluene in relation to changes in the microstructure and size of orange juice particles. J. Funct. Foods, 46, 38-47 (2018).","DOI":"10.1016\/j.jff.2018.04.044"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7) Arain, M.A., Mei, Z., Hassan, F., Saeed, M., Alagawany, M., et al. Lycopene: a natural antioxidant for prevention of heat-induced oxidative stress in poultry. World's Poult. Sci. J., 74, 89-100 (2018).","DOI":"10.1017\/S0043933917001040"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8) Di Mascio, P., Kaiser, S., Sies, H. Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Arch. Biochem. Biophys., 274, 532-538 (1989).","DOI":"10.1016\/0003-9861(89)90467-0"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9) Engelmann, N.J., Clinton, S.K., Erdman, J.W., Jr. Nutritional aspects of phytoene and phytofluene, carotenoid precursors to lycopene. Adv. Nutr., 2, 51-61 (2011).","DOI":"10.3945\/an.110.000075"},{"key":"10","doi-asserted-by":"publisher","unstructured":"10) Iwasaka, H., Koyanagi, R., Satoh, R., Nagano, A., Watanabe, K., et al. A possible trifunctional \u03b2-carotene synthase gene identified in the draft genome of Aurantiochytrium sp. strain KH105. Genes, 9, 200 (2018).","DOI":"10.3390\/genes9040200"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11) Aki, T., Hachida, K., Yoshinaga, M., Katai, Y., Yamasaki, T., et al. Thraustochytrid as a potential source of carotenoids. J. Am. Oil Chem. Soc., 80, 789-794 (2003).","DOI":"10.1007\/s11746-003-0773-2"},{"key":"12","unstructured":"12) Thompson, J.D., Gibson, T.J., Higgins, D.G. Multiple sequence alignment using ClustalW and ClustalX. Curr. Protoc. Bioinf., 1, 2.3.1-2.3.22 (2003)."},{"key":"13","doi-asserted-by":"crossref","unstructured":"13) Bairoch, A., Apweiler, R. The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000. Nucleic Acids Res., 28, 45-48 (2000).","DOI":"10.1093\/nar\/28.1.45"},{"key":"14","doi-asserted-by":"publisher","unstructured":"14) Abramson, J., Adler, J., Dunger, J., Evans, R., Green, T., et al. Accurate structure prediction of biomolecular interactions with AlphaFold 3. Nature, 630, 493-500 (2024).","DOI":"10.1038\/s41586-024-07487-w"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15) Bento, A.P., Hersey, A., F\u00e9lix, E., Landrum, G., Gaulton, A., et al. An open source chemical structure curation pipeline using RDKit. J. Cheminf., 12, 51 (2020).","DOI":"10.1186\/s13321-020-00456-1"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Santos-Martins, D., He, Y., Eberhardt, J., Sharma, P., Bruciaferri, N., et al. Meeko: molecule parametrization and software interoperability for docking and beyond. J. Chem. Inf. Model., 65, 13045-13050 (2025).","DOI":"10.1021\/acs.jcim.5c02271"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17) Pettersen, E.F., Goddard, T.D., Huang, C.C., Meng, E.C., Couch, G.S., et al. UCSF ChimeraX: structure visualization for researchers, educators, and developers. Protein Sci., 30, 70-82 (2021).","DOI":"10.1002\/pro.3943"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18) Trott, O., Olson, A.J. AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J. Comput. Chem., 31, 455-461 (2010).","DOI":"10.1002\/jcc.21334"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19) Ito, H., Fukuda, Y., Murata, K., Kimura, A. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol., 153, 163-168 (1983).","DOI":"10.1128\/jb.153.1.163-168.1983"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20) Watanabe, K., Arafiles, K.H.V., Higashi, R., Okamura, Y., Tajima, T., et al. Isolation of high carotenoid-producing Aurantiochytrium sp. mutants and improvement of astaxanthin productivity using metabolic information. J. Oleo Sci., 67, 571-578 (2018).","DOI":"10.5650\/jos.ess17230"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21) Alqu\u00e9zar, B., Zacar\u00edas, L., Rodrigo, M.J. Molecular and functional characterization of a novel chromoplast-specific lycopene \u03b2-cyclase from Citrus and its relation to lycopene accumulation. J. Exp. Bot., 60, 1783-1797 (2009).","DOI":"10.1093\/jxb\/erp048"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22) Schaub, P., Yu, Q., Gemmecker, S., Poussin-Courmontagne, P., Mailliot, J., et al. On the structure and function of the phytoene desaturase CRTI from Pantoea ananatis, a membrane-peripheral and FAD-dependent oxidase\/isomerase. PLoS One, 7, e39550 (2012).","DOI":"10.1371\/journal.pone.0039550"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23) Guo, W., Tang, H., Zhang, L. Lycopene cyclase and phytoene synthase activities in the marine yeast Rhodosporidium diobovatum are encoded by a single gene crtYB. J. Basic Microbiol., 54, 1053-1061 (2014).","DOI":"10.1002\/jobm.201300920"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24) Zhang, Y., Chen, H., Navarro, E., L\u00f3pez-Garc\u00eda, S., Chen, Y.Q., et al. Generation of lycopene-overproducing strains of the fungus Mucor circinelloides reveals important aspects of lycopene formation and accumulation. Biotechnol. Lett., 39, 439-446 (2017).","DOI":"10.1007\/s10529-016-2265-2"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25) Verwaal, R., Wang, J., Meijnen, J.P., Visser, H., Sandmann, G., et al. High-level production of beta-carotene in Saccharomyces cerevisiae by successive transformation with carotenogenic genes from Xanthophyllomyces dendrorhous. Appl. Environ. Microbiol., 73, 4342-4350 (2007).","DOI":"10.1128\/AEM.02759-06"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26) Rabeharindranto, H., Casta\u00f1o-Cerezo, S., Lautier, T., Garcia-Alles, L.F., Treitz, C., et al. Enzyme-fusion strategies for redirecting and improving carotenoid synthesis in S. cerevisiae. Metab. Eng. Commun., 8, e00086 (2019).","DOI":"10.1016\/j.mec.2019.e00086"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27) Brausemann, A., Gemmecker, S., Koschmieder, J., Ghisla, S., Beyer, P., et al. Structure of phytoene desaturase provides insights into herbicide binding and reaction mechanisms involved in carotene desaturation. Structure, 25, 1222-1232.e1223 (2017).","DOI":"10.1016\/j.str.2017.06.002"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28) Gemmecker, S., Schaub, P., Koschmieder, J., Brausemann, A., Drepper, F., et al. Phytoene desaturase from Oryza sativa: oligomeric assembly, membrane association and preliminary 3D-analysis. PLoS One, 10, e0131717 (2015).","DOI":"10.1371\/journal.pone.0131717"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29) Velayos, A., Eslava, A.P., Iturriaga, E.A. A bifunctional enzyme with lycopene cyclase and phytoene synthase activities is encoded by the carRP gene of Mucor circinelloides. Eur. J. Biochem., 267, 5509-5519 (2000).","DOI":"10.1046\/j.1432-1327.2000.01612.x"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30) Arrach, N., Fern\u00e1ndez-Mart\u00edn, R., Cerd\u00e1-Olmedo, E., Avalos, J. A single gene for lycopene cyclase, phytoene synthase, and regulation of carotene biosynthesis in Phycomyces. Proc. Natl. Acad. Sci. U.S.A., 98, 1687-1692 (2001).","DOI":"10.1073\/pnas.98.4.1687"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31) Watanabe, K., Perez, C.M.T., Kitahori, T., Hata, K., Aoi, M., et al. Improvement of fatty acid productivity of thraustochytrid, Aurantiochytrium sp. by genome editing. J. Biosci. Bioeng., 131, 373-380 (2021).","DOI":"10.1016\/j.jbiosc.2020.11.013"}],"container-title":["Journal of Oleo Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jos\/75\/6\/75_jos.ess26022\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T04:18:56Z","timestamp":1780719536000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jos\/75\/6\/75_jos.ess26022\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.5650\/jos.ess26022","relation":{},"ISSN":["1345-8957","1347-3352"],"issn-type":[{"value":"1345-8957","type":"print"},{"value":"1347-3352","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"jos.ess26022"}}