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Dis. 9, 593-603 (2022).","DOI":"10.1093\/ofid\/ofab593"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3) Miceli, M.H.; D\u00edaz, J.A.; Lee, S.A. Emerging opportunistic yeast infections. Lancet Infect. Dis. 11, 142-151 (2011).","DOI":"10.1016\/S1473-3099(10)70218-8"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4) Munoz; Bouza, E.; Cuenca-Estrella, M.; Eiros, J.M.; P\u00e9rez, M.J., et al. <i>Saccharomyces cerevisiae<\/i> fungemia: an emerging infectious disease. Clin. Infect. Dis. 40, 1625-1634 (2005).","DOI":"10.1086\/429916"},{"key":"5","unstructured":"5) Centers for Disease Control and Prevention. Antibiotic resistance threats in the United States, 2019. US Department of Health and Human Services, Centres for Disease Control and Prevention, (2019)."},{"key":"6","doi-asserted-by":"crossref","unstructured":"6) Cha, R.; Sobel, J.D. Fluconazole for the treatment of candidiasis: 15 years experience. Expert Rev. Anti-Infect. 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Dis. 43, 1069-1070 (2006).","DOI":"10.1086\/507895"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10) Denning, D.W. Invasive aspergillosis. Clin. Infect. Dis. 781-803 (1998).","DOI":"10.1086\/513943"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11) Morizot, G.; Delgiudice, P.; Caumes, E.; Laffitte, E.; Marty, P., et al. Healing of Old World cutaneous leishmaniasis in travelers treated with fluconazole: drug effect or spontaneous evolution? Am. J. Trop. Med. Hyg. 76, 48-52 (2007).","DOI":"10.4269\/ajtmh.2007.76.48"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12) J\u00f8rgensen, L.N.; Heick, T.M. Azole use in agriculture, horticulture, and wood preservation-is it indispensable? Front. Cell. Infect. Microbiol. 730297-730313 (2021).","DOI":"10.3389\/fcimb.2021.730297"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13) Yang, Y.-L.; Lin, C.-C.; Chang, T.-P.; Lauderdale, T.-L.; Chen, H.-T., et al. Comparison of human and soil <i>Candida tropicalis<\/i> isolates with reduced susceptibility to fluconazole. PLoS One 7, 34609-34616 (2012).","DOI":"10.1371\/journal.pone.0034609"},{"key":"14","unstructured":"14) Dun, E. Antifungal resistance in yeast vaginitis. Yale J. Biol. Med. 72, 281-286 (1999)."},{"key":"15","doi-asserted-by":"crossref","unstructured":"15) Rex, J.H.; Rinaldi, M.; Pfaller, M. Resistance of <i>Candida<\/i> species to fluconazole. Antimicrob. Agents Chemother. 39, 1-8 (1995).","DOI":"10.1128\/AAC.39.1.1"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16) Chowdhary, A.; Kathuria, S.; Xu, J.; Meis, J.F. Emergence of azole-resistant <i>Aspergillus fumigatus<\/i> strains due to agricultural azole use creates an increasing threat to human health. PLoS Pathog. 9, 1003633-1003637 (2013).","DOI":"10.1371\/journal.ppat.1003633"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17) Castelo-Branco, D.; Lockhart, S.R.; Chen, Y.C.; Santos, D.A.; Hagen, F., et al. Collateral consequences of agricultural fungicides on pathogenic yeasts: a One Health perspective to tackle azole resistance. Mycoses 65, 303-311 (2022).","DOI":"10.1111\/myc.13404"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18) Chen, T.-C.; Chen, Y.-H.; Chen, Y.-C.; Lu, P.-L. Fluconazole exposure rather than clonal spreading is correlated with the emergence of <i>Candida glabrata<\/i> with cross-resistance to triazole antifungal agents. Kaohsiung J. Med. Sci. 28, 306-315 (2012).","DOI":"10.1016\/j.kjms.2011.11.011"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19) Kelly, S.; Lamb, D.; Kelly, D.; Manning, N.; Loeffler, J., et al. Resistance to fluconazole and cross-resistance to amphotericin B in <i>Candida albicans<\/i> from AIDS patients caused by defective sterol \u03945, 6-desaturation. FEBS Lett. 400, 80-82 (1997).","DOI":"10.1016\/S0014-5793(96)01360-9"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20) Pfaller, M.; Messer, S.; Boyken, L.; Rice, C.; Tendolkar, S., et al. Cross-resistance between fluconazole and ravuconazole and the use of fluconazole as a surrogate marker to predict susceptibility and resistance to ravuconazole among 12,796 clinical isolates of <i>Candida<\/i> spp. J. Clin. Microbiol. 42, 3137-3141 (2004).","DOI":"10.1128\/JCM.42.7.3137-3141.2004"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21) Perlin, D.S.; Rautemaa-Richardson, R.; Alastruey-Izquierdo, A. The global problem of antifungal resistance: prevalence, mechanisms, and management. Lancet Infect. Dis. 17, 383-392 (2017).","DOI":"10.1016\/S1473-3099(17)30316-X"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22) Hendrickson, J.A.; Hu, C.; Aitken, S.L.; Beyda, N. Antifungal resistance: a concerning trend for the present and future. Curr. Infect. Dis. Rep. 21, 1-8 (2019).","DOI":"10.1007\/s11908-019-0702-9"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23) Ji, C.; Zhou, Y.; Leblanc, R.M.; Peng, Z. Recent developments of carbon dots in biosensing: a review. ACS Sens. 5, 2724-2741 (2020).","DOI":"10.1021\/acssensors.0c01556"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24) Zhou, Y.; Sharma, S.K.; Peng, Z.; Leblanc, R.M. Polymers in carbon dots: a review. Polymers 9, 67-86 (2017).","DOI":"10.3390\/polym9020067"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25) Xia, C.; Zhu, S.; Feng, T.; Yang, M.; Yang, B. Evolution and synthesis of carbon dots: from carbon dots to carbonized polymer dots. Adv. Sci. 6, 1901316-1901338 (2019).","DOI":"10.1002\/advs.201901316"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26) Khayal, A.; Dawane, V.; Amin, M.A.; Tirth, V.; Yadav, V.K., et al. Advances in the methods for the synthesis of carbon dots and their emerging applications. Polymers 13, 3190-3221 (2021).","DOI":"10.3390\/polym13183190"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27) de Medeiros, T.V.; Manioudakis, J.; Noun, F.; Macairan, J.-R.; Victoria, F.; Naccache, R. Microwave-assisted synthesis of carbon dots and their applications. J. Mater. Chem. C 7, 7175-7195 (2019).","DOI":"10.1039\/C9TC01640F"},{"key":"28","doi-asserted-by":"publisher","unstructured":"28) Jorns, M.; Pappas, D. A review of fluorescent carbon dots, their synthesis, physical and chemical characteristics, and applications. Nanomaterials 11, 1448-1466 (2021).","DOI":"10.3390\/nano11061448"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29) Rocco, D.; Moldoveanu, V.G.; Feroci, M.; Bortolami, M.; Vetica, F. Electrochemical synthesis of carbon quantum dots. ChemElectroChem 10, e202201104 (2023).","DOI":"10.1002\/celc.202201104"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30) Boakye-Yiadom, K.O.; Kesse, S.; Opoku-Damoah, Y.; Filli, M.S.; Aquib, M., et al. Carbon dots: applications in bioimaging and theranostics. Int. J. Pharm. 564, 308-317 (2019).","DOI":"10.1016\/j.ijpharm.2019.04.055"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31) Rajkumar, M.; Begum, M.Y.; Al Hamod, M.; Alotaibi, H.; Al Hamood, N., et al. Biosynthesis of chitosan-TiO<sub>2<\/sub> nanoparticles using <i>Phyllanthus niruri<\/i> (L.) extract: in vitro evaluation of their antioxidant, antibacterial, anti-inflammatory and anticancer properties. Inorg. Chem. Commun. 115843 (2025).","DOI":"10.1016\/j.inoche.2025.115843"},{"key":"32","unstructured":"32) Kiguchi, M.; Wang, J.; Yuxin, L.; Zhang, D.; Liang, Z., et al. Nano-sized magnetic TiO<sub>2<\/sub>-SiO<sub>2<\/sub>\/Fe<sub>3<\/sub>O<sub>4<\/sub> photocatalysts and their application to photokilling of <i>Lactobacillus casei<\/i> and photocatalytic degradation of persistent herbicides. Bull. Chem. Soc. Jpn. 98, 95 (2025)."},{"key":"33","doi-asserted-by":"crossref","unstructured":"33) El-Khawaga, A.M.; Elsayed, M.A.; Gobara, M.; Suliman, A.A.; Hashem, A.H., et al. Green synthesized ZnO nanoparticles by <i>Saccharomyces cerevisiae<\/i> and their antibacterial activity and photocatalytic degradation. Biomass Convers. Biorefin. 15, 2673-2684 (2025).","DOI":"10.1007\/s13399-023-04827-0"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34) Dong, X.; Liang, W.; Meziani, M.J.; Sun, Y.-P.; Yang, L. Carbon dots as potent antimicrobial agents. Theranostics 10, 671-686 (2020).","DOI":"10.7150\/thno.39863"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35) Maruthapandi, M.; Saravanan, A.; Das, P.; Luong, J.H.; Gedanken, A. Microbial inhibition and biosensing with multifunctional carbon dots: progress and perspectives. Biotechnol. Adv. 53, 107843-107858 (2021).","DOI":"10.1016\/j.biotechadv.2021.107843"},{"key":"36","doi-asserted-by":"publisher","unstructured":"36) Ghirardello, M.; Ramos-Soriano, J.; Galan, M.C. Carbon dots as an emergent class of antimicrobial agents. Nanomaterials 11, 1877-1900 (2021).","DOI":"10.3390\/nano11081877"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37) Thakur, M.; Pandey, S.; Mewada, A.; Patil, V.; Khade, M., et al. Antibiotic conjugated fluorescent carbon dots as a theranostic agent for controlled drug release, bioimaging, and enhanced antimicrobial activity. J. Drug Deliv. 2014, 282193-282201 (2014).","DOI":"10.1155\/2014\/282193"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38) Phan, L.M.T.; Cho, S. Fluorescent carbon dot-supported imaging-based biomedicine: a comprehensive review. Bioinorg. Chem. Appl. 2022, 9303703 (2022).","DOI":"10.1155\/2022\/9303703"},{"key":"39","doi-asserted-by":"publisher","unstructured":"39) Jin, X.; Sun, X.; Chen, G.; Ding, L.; Li, Y., et al. pH-sensitive carbon dots for the visualization of regulation of intracellular pH inside living pathogenic fungal cells. Carbon 81, 388-395 (2015).","DOI":"10.1016\/j.carbon.2014.09.071"},{"key":"40","doi-asserted-by":"crossref","unstructured":"40) Kasibabu, B.S.B.; D&apos;souza, S.L.; Jha, S.; Singhal, R.K.; Basu, H.; Kailasa, S.K. One-step synthesis of fluorescent carbon dots for imaging bacterial and fungal cells. Anal. Methods 7, 2373-2378 (2015).","DOI":"10.1039\/C4AY02737J"},{"key":"41","doi-asserted-by":"crossref","unstructured":"41) Ezati, P.; Rhim, J.-W.; Molaei, R.; Priyadarshi, R.; Roy, S., et al. Preparation and characterization of B, S, and N-doped glucose carbon dots: antibacterial, antifungal, and antioxidant activity. Sustain. Mater. Technol. 32, 397-408 (2022).","DOI":"10.1016\/j.susmat.2022.e00397"},{"key":"42","doi-asserted-by":"crossref","unstructured":"42) Gao, Z.; Li, X.; Shi, L.; Yang, Y. Deep eutectic solvents-derived carbon dots for detection of mercury (II), photocatalytic antifungal activity and fluorescent labeling for <i>C. albicans<\/i>. Spectrochim. Acta A Mol. Biomol. Spectrosc. 220, 117080-117088 (2019).","DOI":"10.1016\/j.saa.2019.04.072"},{"key":"43","doi-asserted-by":"crossref","unstructured":"43) Li, X.; Wu, X.; Yuan, T.; Zhu, J.; Yang, Y. Influence of the iodine content of nitrogen-and iodine-doped carbon dots as a peroxidase mimetic nanozyme exhibiting antifungal activity against <i>C. albicans<\/i>. Biochem. Eng. J. 175, 108139-108147 (2021).","DOI":"10.1016\/j.bej.2021.108139"},{"key":"44","doi-asserted-by":"crossref","unstructured":"44) Priyadarshini, E.; Rawat, K.; Prasad, T.; Bohidar, H. Antifungal efficacy of Au@ carbon dots nanoconjugates against opportunistic fungal pathogen, <i>Candida albicans<\/i>. Colloids Surf. B Biointerfaces 163, 355-361 (2018).","DOI":"10.1016\/j.colsurfb.2018.01.006"},{"key":"45","doi-asserted-by":"crossref","unstructured":"45) Sturabotti, E.; Camilli, A.; Moldoveanu, V.G.; Bonincontro, G.; Simonetti, G., et al. Targeting the antifungal activity of carbon dots against <i>Candida albicans<\/i> biofilm formation by tailoring their surface functional groups. Chem. Eur. J. 30, e202303631 (2024).","DOI":"10.1002\/chem.202303631"},{"key":"46","doi-asserted-by":"crossref","unstructured":"46) Fryberg, M.; Oehlschlager, A.C.; Unrau, A. Biosynthesis of ergosterol in yeast. Evidence for multiple pathways. J. Am. Chem. Soc. 95, 5747-5757 (1973).","DOI":"10.1021\/ja00798a051"},{"key":"47","doi-asserted-by":"crossref","unstructured":"47) Anderson, I.C.; Cairney, J.W. Diversity and ecology of soil fungal communities: increased understanding through the application of molecular techniques. Environ. Microbiol. 6, 769-779 (2004).","DOI":"10.1111\/j.1462-2920.2004.00675.x"},{"key":"48","doi-asserted-by":"crossref","unstructured":"48) Raghavan, V.; Aquadro, C.F.; Alani, E. Baker&apos;s yeast clinical isolates provide a model for how pathogenic yeasts adapt to stress. Trends Genet. 35, 804-817 (2019).","DOI":"10.1016\/j.tig.2019.08.002"},{"key":"49","doi-asserted-by":"crossref","unstructured":"49) Revie, N.M.; Iyer, K.R.; Robbins, N.; Cowen, L.E. Antifungal drug resistance: evolution, mechanisms and impact. Curr. Opin. Microbiol. 45, 70-76 (2018).","DOI":"10.1016\/j.mib.2018.02.005"},{"key":"50","doi-asserted-by":"crossref","unstructured":"50) Lenski, R.E.; Rose, M.R.; Simpson, S.C.; Tadler, S.C. Long-term experimental evolution in <i>Escherichia coli<\/i>. I. Adaptation and divergence during 2,000 generations. Am. Nat. 138, 1315-1341 (1991).","DOI":"10.1086\/285289"},{"key":"51","doi-asserted-by":"crossref","unstructured":"51) Blount, Z.D.; Borland, C.Z.; Lenski, R.E. Historical contingency and the evolution of a key innovation in an experimental population of <i>Escherichia coli<\/i>. Proc. Natl. Acad. Sci. U. S. A. 105, 7899-7906 (2008).","DOI":"10.1073\/pnas.0803151105"},{"key":"52","doi-asserted-by":"crossref","unstructured":"52) Kawecki, T.; Lenski, R.; Ebert, D.; Hollis, B.; Olivieri, I.; Whitlock, M. Experimental evolution. Trends Ecol. Evol. 27, 547-560 (2012).","DOI":"10.1016\/j.tree.2012.06.001"},{"key":"53","unstructured":"53) Mart\u00ednez, A.A.; Lang, G.I. Identifying targets of selection in laboratory evolution experiments. J. Mol. Evol. 1-11 (2023)."},{"key":"54","doi-asserted-by":"crossref","unstructured":"54) Marichal, P.; Vanden Bossche, H.; Odds, F.C.; Nobels, G.; Warnock, D.W., et al. Molecular biological characterization of an azole-resistant <i>Candida glabrata<\/i> isolate. Antimicrob. Agents Chemother. 41, 2229-2237 (1997).","DOI":"10.1128\/AAC.41.10.2229"},{"key":"55","doi-asserted-by":"crossref","unstructured":"55) Cowen, L.E.; Sanglard, D.; Calabrese, D.; Sirjusingh, C.; Anderson, J.B.; Kohn, L.M. Evolution of drug resistance in experimental populations of <i>Candida albicans<\/i>. J. Bacteriol. 182, 1515-1522 (2000).","DOI":"10.1128\/JB.182.6.1515-1522.2000"},{"key":"56","doi-asserted-by":"crossref","unstructured":"56) Osset-Tr\u00e9nor, P.; Pascual-Ahuir, A.; Proft, M. Fungal drug response and antimicrobial resistance. J. Fungi 9, 565 (2023).","DOI":"10.3390\/jof9050565"},{"key":"57","doi-asserted-by":"crossref","unstructured":"57) Todd, R.T.; Soisangwan, N.; Peters, S.; Kemp, B.; Crooks, T., et al. Antifungal drug concentration impacts the spectrum of adaptive mutations in <i>Candida albicans<\/i>. Mol. Biol. Evol. 40, 9-41 (2023).","DOI":"10.1093\/molbev\/msad009"},{"key":"58","doi-asserted-by":"crossref","unstructured":"58) Yu, D.T.; Peterson, J.F.; Seger, D.L.; Gerth, W.C.; Bates, D.W. Frequency of potential azole drug-drug interactions and consequences of potential fluconazole drug interactions. Pharmacoepidemiol. Drug Saf. 14, 755-767 (2005).","DOI":"10.1002\/pds.1073"},{"key":"59","doi-asserted-by":"crossref","unstructured":"59) Kowalsky, S.F. Drug interactions with fluconazole. Pharmacotherapy 10, 170-173 (1990).","DOI":"10.1002\/j.1875-9114.1990.tb02605.x"},{"key":"60","doi-asserted-by":"crossref","unstructured":"60) Choi, Y.; Choi, Y.; Kwon, O.H.; Kim, B.S. Carbon dots: bottom-up syntheses, properties, and light-harvesting applications. Chem. Asian J. 13, 586-598 (2018).","DOI":"10.1002\/asia.201701736"},{"key":"61","doi-asserted-by":"crossref","unstructured":"61) Zhou, Y.; Mintz, K.J.; Cheng, L.; Chen, J.; Ferreira, B.C., et al. Direct conjugation of distinct carbon dots as Lego-like building blocks for the assembly of versatile drug nanocarriers. J. Colloid Interface Sci. 576, 412-425 (2020).","DOI":"10.1016\/j.jcis.2020.05.005"},{"key":"62","doi-asserted-by":"crossref","unstructured":"62) Modi, P.D.; Mehta, V.N.; Prajapati, V.S.; Patel, S.; Rohit, J.V. Bottom-up approaches for the preparation of carbon dots. in Carbon Dots in Analytical Chemistry, Elsevier, pp. 15-29 (2023).","DOI":"10.1016\/B978-0-323-98350-1.00022-0"},{"key":"63","doi-asserted-by":"publisher","unstructured":"63) Li, S.; Amat, D.; Peng, Z.; Vanni, S.; Raskin, S., et al. Transferrin conjugated nontoxic carbon dots for doxorubicin delivery to target pediatric brain tumor cells. Nanoscale 8, 16662-16669 (2016).","DOI":"10.1039\/C6NR05055G"},{"key":"64","doi-asserted-by":"crossref","unstructured":"64) Zhou, Y.; Desserre, A.; Sharma, S.K.; Li, S.; Marksberry, M.H., et al. Gel-like carbon dots: characterization and their potential applications. ChemPhysChem 18, 890-897 (2017).","DOI":"10.1002\/cphc.201700038"},{"key":"65","doi-asserted-by":"crossref","unstructured":"65) Seven, E.S.; Seven, Y.B.; Zhou, Y.; Poudel-Sharma, S.; Diaz-Rucco, J.J., et al. Crossing the blood-brain barrier with carbon dots: uptake mechanism and in vivo cargo delivery. Nanoscale Adv. 3, 3942-3953 (2021).","DOI":"10.1039\/D1NA00145K"},{"key":"66","unstructured":"66) Protocol, A.G. CellTiter 96\u00ae AQueous One Solution Cell Proliferation Assay. Promega, USA, (2001)."},{"key":"67","doi-asserted-by":"publisher","unstructured":"67) Malich, G.; Markovic, B.; Winder, C. The sensitivity and specificity of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines. Toxicology 124, 179-192 (1997).","DOI":"10.1016\/S0300-483X(97)00151-0"},{"key":"68","doi-asserted-by":"crossref","unstructured":"68) Broekaert, W.F.; Terras, F.R.G.; Cammue, B.P.A.; Vanderleyden, J. An automated quantitative assay for fungal growth inhibition. FEMS Microbiol. Lett. 69, 55-59 (1990).","DOI":"10.1111\/j.1574-6968.1990.tb04174.x"},{"key":"69","doi-asserted-by":"crossref","unstructured":"69) Cherene, M.B.; Taveira, G.B.; Almeida-Silva, F.; Silva, M.S.D.; Cavaco, M.C., et al. Structural and biochemical characterization of three antimicrobial peptides from <i>Capsicum annuum<\/i> L. Var. annuum leaves for anti-<i>Candida<\/i> use. Probiotics Antimicrob. Proteins 1-18 (2023).","DOI":"10.1007\/s12602-023-10112-3"},{"key":"70","doi-asserted-by":"crossref","unstructured":"70) Raymaekers, K.; Ponet, L.; Holtappels, D.; Berckmans, B.; Cammue, B.P. Screening for novel biocontrol agents applicable in plant disease management-a review. Biol. Control 144, 104240-104257 (2020).","DOI":"10.1016\/j.biocontrol.2020.104240"},{"key":"71","doi-asserted-by":"crossref","unstructured":"71) Zhang, B.; Kula, A.; Mack, K.M.; Zhai, L.; Ryce, A.L., et al. Carrying capacity in a heterogeneous environment with habitat connectivity. Ecol. Lett. 20, 1118-1128 (2017).","DOI":"10.1111\/ele.12807"},{"key":"72","doi-asserted-by":"crossref","unstructured":"72) Xu, S.; Van Dyken, J.D. Microbial expansion-collision dynamics promote cooperation and coexistence on surfaces. Evolution 72, 153-169 (2018).","DOI":"10.1111\/evo.13393"},{"key":"73","doi-asserted-by":"crossref","unstructured":"73) Lang, G.I.; Botstein, D.; Desai, M.M. Genetic variation and the fate of beneficial mutations in asexual populations. Genetics 188, 647-661 (2011).","DOI":"10.1534\/genetics.111.128942"},{"key":"74","doi-asserted-by":"crossref","unstructured":"74) Paudyal, S.; Vallejo, F.A.; Cilingir, E.K.; Zhou, Y.; Mintz, K.J., et al. DFMO carbon dots for treatment of neuroblastoma and bioimaging. ACS Appl. Bio Mater. 5, 3300-3309 (2022).","DOI":"10.1021\/acsabm.2c00309"},{"key":"75","doi-asserted-by":"crossref","unstructured":"75) Mintz, K.J.; Bartoli, M.; Rovere, M.; Zhou, Y.; Hettiarachchi, S.D., et al. A deep investigation into the structure of carbon dots. Carbon 173, 433-447 (2021).","DOI":"10.1016\/j.carbon.2020.11.017"},{"key":"76","doi-asserted-by":"crossref","unstructured":"76) Anderson, J.B.; Sirjusingh, C.; Parsons, A.B.; Boone, C.; Wickens, C., et al. Mode of selection and experimental evolution of antifungal drug resistance in <i>Saccharomyces cerevisiae<\/i>. Genetics 163, 1287-1298 (2003).","DOI":"10.1093\/genetics\/163.4.1287"},{"key":"77","doi-asserted-by":"crossref","unstructured":"77) Dong, X.; Awak, M.A.; Tomlinson, N.; Tang, Y.; Sun, Y.-P.; Yang, L. Antibacterial effects of carbon dots in combination with other antimicrobial reagents. PLoS One 12, 185324-185339 (2017).","DOI":"10.1371\/journal.pone.0185324"},{"key":"78","doi-asserted-by":"crossref","unstructured":"78) Saravanan, A.; Maruthapandi, M.; Das, P.; Ganguly, S.; Margel, S., et al. Applications of N-doped carbon dots as antimicrobial agents, antibiotic carriers, and selective fluorescent probes for nitro explosives. ACS Appl. Bio Mater. 3, 8023-8031 (2020).","DOI":"10.1021\/acsabm.0c01104"},{"key":"79","doi-asserted-by":"crossref","unstructured":"79) Alas, M.O.; Alkas, F.B.; Sukuroglu, A.A.; Alturk, R.G.; Battal, D. Fluorescent carbon dots are the new quantum dots: an overview of their potential in emerging technologies and nanosafety. J. Mater. Sci. 55, 15074-15105 (2020).","DOI":"10.1007\/s10853-020-05054-y"},{"key":"80","doi-asserted-by":"crossref","unstructured":"80) Ge, G.; Li, L.; Wang, D.; Chen, M.; Zeng, Z., et al. Carbon dots: synthesis, properties and biomedical applications. J. Mater. Chem. B 9, 6553-6575 (2021).","DOI":"10.1039\/D1TB01077H"},{"key":"81","doi-asserted-by":"crossref","unstructured":"81) \u00d0or\u0111evi\u0107, L.; Arcudi, F.; Cacioppo, M.; Prato, M. A multifunctional chemical toolbox to engineer carbon dots for biomedical and energy applications. Nat. Nanotechnol. 17, 112-130 (2022).","DOI":"10.1038\/s41565-021-01051-7"},{"key":"82","doi-asserted-by":"crossref","unstructured":"82) Yu, M.; Li, P.; Huang, R.; Xu, C.; Zhang, S., et al. Antibacterial and antibiofilm mechanisms of carbon dots: a review. J. Mater. Chem. B 11, 734-754 (2023).","DOI":"10.1039\/D2TB01977A"},{"key":"83","doi-asserted-by":"crossref","unstructured":"83) Liu, Y.; Li, W.; Wu, K.; Lei, B.; Chen, J., et al. Antifungal molecular details of MNQ-derived novel carbon dots against <i>Penicillium digitatum<\/i>. Food Chem. 413, 135687-135695 (2023).","DOI":"10.1016\/j.foodchem.2023.135687"},{"key":"84","doi-asserted-by":"crossref","unstructured":"84) Ezati, P.; Rhim, J.-W.; Molaei, R.; Rezaei, Z. Carbon quantum dots-based antifungal coating film for active packaging application of avocado. Food Packag. Shelf Life 33, 100878-100887 (2022).","DOI":"10.1016\/j.fpsl.2022.100878"},{"key":"85","doi-asserted-by":"publisher","unstructured":"85) Lin, F.; Wang, Z.; Wu, F.-G. Carbon dots for killing microorganisms: an update since 2019. Pharmaceuticals 15, 1236-1255 (2022).","DOI":"10.3390\/ph15101236"},{"key":"86","doi-asserted-by":"publisher","unstructured":"86) Yin, R.; Agrawal, T.; Khan, U.; Gupta, G.K.; Rai, V., et al. Antimicrobial photodynamic inactivation in nanomedicine: small light strides against bad bugs. Nanomedicine 10, 2379-2404 (2015).","DOI":"10.2217\/nnm.15.67"},{"key":"87","doi-asserted-by":"crossref","unstructured":"87) Cieplik, F.; Deng, D.; Crielaard, W.; Buchalla, W.; Hellwig, E., et al. Antimicrobial photodynamic therapy-what we know and what we don&apos;t. Crit. Rev. Microbiol. 44, 571-589 (2018).","DOI":"10.1080\/1040841X.2018.1467876"},{"key":"88","doi-asserted-by":"publisher","unstructured":"88) LeCroy, G.E.; Sonkar, S.K.; Yang, F.; Veca, L.M.; Wang, P., et al. Toward structurally defined carbon dots as ultracompact fluorescent probes. ACS Nano 8, 4522-4529 (2014).","DOI":"10.1021\/nn406628s"},{"key":"89","doi-asserted-by":"crossref","unstructured":"89) Luo, P.G.; Sahu, S.; Yang, S.-T.; Sonkar, S.K.; Wang, J., et al. Carbon \u201cquantum\u201d dots for optical bioimaging. J. Mater. Chem. B 1, 2116-2127 (2013).","DOI":"10.1039\/c3tb00018d"},{"key":"90","doi-asserted-by":"publisher","unstructured":"90) Jian, H.-J.; Wu, R.-S.; Lin, T.-Y.; Li, Y.-J.; Lin, H.-J., et al. Super-cationic carbon quantum dots synthesized from spermidine as an eye drop formulation for topical treatment of bacterial keratitis. ACS Nano 11, 6703-6716 (2017).","DOI":"10.1021\/acsnano.7b01023"},{"key":"91","doi-asserted-by":"publisher","unstructured":"91) Lin, F.; Bao, Y.-W.; Wu, F.-G. Carbon dots for sensing and killing microorganisms. C 5, 33-53 (2019).","DOI":"10.3390\/c5020033"},{"key":"92","doi-asserted-by":"crossref","unstructured":"92) Dong, X.; Moyer, M.M.; Yang, F.; Sun, Y.-P.; Yang, L. Carbon dots&apos; antiviral functions against noroviruses. Sci. Rep. 7, 519-528 (2017).","DOI":"10.1038\/s41598-017-00675-x"},{"key":"93","doi-asserted-by":"crossref","unstructured":"93) Wang, B.; Cai, H.; Waterhouse, G.I.; Qu, X.; Yang, B.; Lu, S. Carbon dots in bioimaging, biosensing and therapeutics: a comprehensive review. Small Sci. 2, 12-37 (2022).","DOI":"10.1002\/smsc.202200012"},{"key":"94","doi-asserted-by":"crossref","unstructured":"94) Andersson, D.I.; Patin, S.M.; Nilsson, A.I.; Kugelberg, E. The biological cost of antibiotic resistance. in Enzyme-Mediated Resistance to Antibiotics: Mechanisms, Dissemination, and Prospects for Inhibition, pp. 339-348 (2007).","DOI":"10.1128\/9781555815615.ch21"},{"key":"95","unstructured":"95) Lenski, R.E. Bacterial evolution and the cost of antibiotic resistance. Int. Microbiol. 1, 265-270 (1998)."},{"key":"96","doi-asserted-by":"crossref","unstructured":"96) Dean, Z.; Maltas, J.; Wood, K.B. Antibiotic interactions shape short-term evolution of resistance in <i>E. faecalis<\/i>. PLoS Pathog. 16, 1008278-10082101 (2020).","DOI":"10.1371\/journal.ppat.1008278"},{"key":"97","doi-asserted-by":"crossref","unstructured":"97) Anderson, J.B. Evolution of antifungal-drug resistance: mechanisms and pathogen fitness. Nat. Rev. Microbiol. 3, 547-556 (2005).","DOI":"10.1038\/nrmicro1179"},{"key":"98","doi-asserted-by":"crossref","unstructured":"98) Cowen, L.E.; Kohn, L.M.; Anderson, J.B. Divergence in fitness and evolution of drug resistance in experimental populations of <i>Candida albicans<\/i>. J. Bacteriol. 183, 2971-2978 (2001).","DOI":"10.1128\/JB.183.10.2971-2978.2001"},{"key":"99","doi-asserted-by":"publisher","unstructured":"99) Paquin, C.E.; Adams, J. Relative fitness can decrease in evolving asexual populations of <i>S. cerevisiae<\/i>. Nature 306, 368-371 (1983).","DOI":"10.1038\/306368a0"},{"key":"100","doi-asserted-by":"crossref","unstructured":"100) Mann, R.; Holmes, A.; McNeilly, O.; Cavaliere, R.; Sotiriou, G.A., et al. Evolution of biofilm-forming pathogenic bacteria in the presence of nanoparticles and antibiotic: adaptation phenomena and cross-resistance. J. 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