{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:44:50Z","timestamp":1785797090383,"version":"3.56.0"},"reference-count":34,"publisher":"The Korean Society for Reproductive Medicine","issue":"2","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["ecerm.org"],"crossmark-restriction":true},"short-container-title":["Clin Exp Reprod Med"],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:p>Objective: The present study investigated sperm chromatin quality and testosterone levels in acrylamide-treated mice and the possible protective effects of vitamin E on the fertility potential of spermatozoa. Methods: Thirty-two adult male mice were divided equally into four groups. Group 1 was the control, group 2 received acrylamide (10 mg\/kg, water solution), group 3 received vitamin E (100 mg\/kg, intraperitoneal), and group 4 received both acrylamide and vitamin E. After 35 days, spermatozoa from the right cauda epididymis were analyzed in terms of count, motility, morphology, and viability. Sperm DNA integrity and chromatin condensation were assessed by acridine orange (AO), aniline blue (AB), toluidine blue (TB), and chromomycin A3 (CMA3) staining. Results: In acrylamide-treated mice, significantly lower sperm concentration, viability, motility, and testosterone levels were found in comparison with the control and acrylamide+vitamin E groups (&lt;i&gt;p&lt;\/i&gt;&lt;0.05). In the vitamin E group, significantly more favorable sperm parameters and testosterone levels were found than in the other groups (&lt;i&gt;p&lt;\/i&gt;&lt;0.05). There were also significantly more spermatozoa with less condensed chromatin in the acrylamide-treated mice than in the other groups. Moreover, significantly more spermatozoa with mature nuclei (assessed by AB, CMA3, AO, and TB staining) were present in the vitamin E group than in the control and acrylamide+vitamin E groups. Conclusion: This study revealed the deleterious effects of acrylamide on sperm parameters and sperm chromatin quality. Vitamin E can not only compensate for the toxic effects of acrylamide, but also improve sperm chromatin quality in mice.<\/jats:p>","DOI":"10.5653\/cerm.2019.03230","type":"journal-article","created":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T02:36:32Z","timestamp":1591065392000},"page":"101-107","update-policy":"https:\/\/doi.org\/10.5653\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Effects of acrylamide in the presence of vitamin E on sperm parameters, chromatin quality, and testosterone levels in mice"],"prefix":"10.5653","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3114-9513","authenticated-orcid":true,"given":"Morteza","family":"Anvari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4883-3020","authenticated-orcid":true,"given":"Ali Reza","family":"Talebi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6206-7831","authenticated-orcid":true,"given":"Esmat","family":"Mangoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9445-3806","authenticated-orcid":true,"given":"Abbas","family":"Shahedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5106-2768","authenticated-orcid":true,"given":"Mohammad Rasool","family":"Ghasemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7549-4672","authenticated-orcid":true,"given":"Majid","family":"Pourentezari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3624","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1080\/19440049.2018.1508893"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2016.1215289"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-019-02741-4"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1039\/C6FO01553K"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2017.12.001"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2018.03.027"},{"key":"ref7","first-page":"335","volume-title":"Effects of acrylamide on sperm parameters, chromatin quality, and the level of blood testosterone in mice","author":"Pourentezari","year":"2014"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmau.2017.03.003"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111\/and.13012"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-016-9566-5"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111\/and.13193"},{"key":"ref12","first-page":"589","volume-title":"Antagonistic effect of vitamin E on di-2-ethylhexyl phthalate-induced reproductive toxicity in male rats","author":"Wang","year":"2018"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.3109\/14647273.2015.1050462"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012274206337"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.29252\/ijrm.16.6.365"},{"key":"ref16","volume-title":"WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction","year":"1999"},{"key":"ref17","first-page":"249","volume-title":"Effect of vitamin E on sperm parameters and DNA integrity in sodium arsenite-treated rats","author":"Momeni","year":"2012"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.29252\/ijrm.15.3.161"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1111\/and.12481"},{"key":"ref20","first-page":"53","volume-title":"Effects of experimentally-induced diabetes on sperm parameters and chromatin quality in mice","author":"Mangoli","year":"2013"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(96)00313-5"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2004.10.006"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.3109\/15376510903559968"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2009.03.001"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.3390\/nu6020466"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.4067\/S0717-95022011000400003"},{"key":"ref27","first-page":"29","volume-title":"Correlation of sperm morphology and oxidative stress in infertile men","author":"Venkatesh","year":"2009"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2003.10.007"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.01.024"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2006.11.003"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2011.59"},{"key":"ref32","first-page":"307","volume-title":"Saccharin consumption increases sperm DNA fragmentation and apoptosis in mice","author":"Rahimipour","year":"2014"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2014.07.007"},{"key":"ref34","first-page":"35","volume-title":"Comparing the roles of sperm chromatin integrity and apoptosis in intrauterine insemination outcomes of couples with mild male and female factor infertility","author":"Khalili","year":"2014"}],"container-title":["Clinical and Experimental Reproductive Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ecerm.org\/upload\/pdf\/cerm-2019-03230.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/ecerm.org\/journal\/view.php?doi=10.5653\/cerm.2019.03230","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/ecerm.org\/upload\/pdf\/cerm-2019-03230.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,11]],"date-time":"2020-06-11T00:38:32Z","timestamp":1591835912000},"score":1,"resource":{"primary":{"URL":"http:\/\/ecerm.org\/journal\/view.php?doi=10.5653\/cerm.2019.03230"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,1]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,6]]}},"URL":"https:\/\/doi.org\/10.5653\/cerm.2019.03230","relation":{},"ISSN":["2233-8233","2233-8241"],"issn-type":[{"value":"2233-8233","type":"print"},{"value":"2233-8241","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,1]]},"assertion":[{"value":"2019-08-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-01","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-01","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}